Cacna1d (NM_028981) Mouse Tagged ORF Clone

SKU MR222925

Cacna1d (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cacna1d
• Synonyms: 8430418G19Rik; C79217; Cach3; Cacn; Cacn4; Cacnl1a2; Cav1.3; Cchl; Cchl1; Cchl1a; Cchl1a2; D-LTC; D-LTCC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222925 representing NM_028981; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAACCTTCCGACATTTTCTAGTGATCTAATTTTAATTAAAAGCGTTCTCTCCCAAGAGACTGATGCCC; GATATAAAGGCAGAGTTGTAAGTGCGGTAGAAAGCACGGAGGACTTCTCCCAGGCCTTTGCAGAGGCAAA; CTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCCAACAGCTCCAAGCAA; ACTGTCCTATCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGCACCT; CTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAACAGGG; TAACTCGTCTAACAGCCGACCTGCCCGTGCCCTTTTCTGTTTATCCCTCAATAACCCCATCCGAAGAGCC; TGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGTGTGG; CCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAGTAGA; ATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTATGGACTGTTGCTGCAT; CCTAATGCTTACGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATTGTAGGATTGTTTAGTG; TAATTCTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCTTTGA; TGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCACTTCGACTTGTATCAGGAGTGCCCAGTTTACAA; GTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTTCACATAGCCCTTTTGGTATTATTTGTAA; TTATCATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTGCTGA; CTCAGATATAGTAGCTGAAGAGGACCCAGCTCCGTGTGCGTTCTCAGGGAATGGACGCCAGTGCACCGCC; AATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCCAATGGAGGCATCACTAACTTTGATAACTTTGCCT; TTGCCATGCTCACTGTGTTCCAGTGCATTACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTAATGA; TGCGATAGGATGGGAATGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGTCCTT; AACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCCAAGGAAAGAGAGAAGGCTAAAGCTCGTGGAGATT; TCCAGAAGCTTCGGGAAAAACAGCAGCTGGAAGAGGACCTCAAGGGCTACCTGGACTGGATCACCCAGGC; AGAAGACATCGATCCCGAGAACGAGGAGGAGGGTGGCGAGGAAGGCAAACGAAACACTAGCATGCCCACC; AGCGAGACTGAATCTGTCAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGAACTC; TCTGTCAGGCCATCTCGAAATCCAAACTCAGCCGTCGATGGCGTCGCTGGAACCGGTTCAATCGCAGGAG; GTGCAGGGCTGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTGATTGTCCTGGTGTTTCTCAACACTTTA; ACTATTTCCTCTGAGCATTACAATCAGCCAGACTGGCTGACACAGATTCAAGACATCGCAAACAAAGTCC; TCTTGGCTCTGTTCACCTGTGAGATGCTGGTGAAGATGTACAGCCTGGGCCTCCAGGCTTACTTTGTCTC; CCTCTTCAACCGCTTCGATTGCTTTGTGGTGTGTGGGGGCATCACCGAAACCATCCTGGTGGAACTGGAG; CTCATGTCTCCCCTGGGGGTCTCCGTGTTCCGGTGTGTGCGCCTCCTGAGAATCTTCAAAGTGACCAGGC; ACTGGACCTCCCTGAGCAACTTGGTGGCATCCTTGTTAAACTCCATGAAGTCCATCGCCTCTCTGCTGTT; ACTCCTCTTCCTCTTCATCATCATCTTCTCCTTGCTGGGGATGCAGTTGTTTGGTGGGAAGTTTAATTTT; GACGAGACTCAAACCAAGCGAAGCACCTTTGACAACTTTCCGCAAGCACTCCTGACGGTGTTCCAGATCC; TGACAGGTGAAGACTGGAATGCGGTGATGTATGACGGCATCATGGCCTATGGAGGCCCTTCCTCTTCCGG; GATGATTGTCTGTATCTACTTCATCATCCTTTTCATCTGTGGTAACTATATCCTGCTGAATGTCTTCTTG; GCCATCGCTGTGGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCTGAAGAAA; AAGAAAGGAAAAAGATTGCCAGAAAAGAAAGCCTAGAAAACAAAAAGAACAACAAACCAGAAGTCAACCA; GATAGCCAACAGTGACAACAAGGTTACAATTGATGATTATCAGGAAGATGCTGAAGACAAGGACCCTTAC; CCGCCCTGTGATGTGCCAGTAGGTGAAGAGGAAGAGGAGGAGGAAGAGGATGAGCCCGAAGTTCCTGCTG; GTCCCCGCCCTCGCAGAATCTCAGAGTTGAACATGAAGGAGAAGATTGCGCCCATCCCAGAAGGAAGCGC; CTTCTTCATTCTTAGCAAGACCAACCCGATACGTGTGGGTTGCCACAAGCTCATTAACCACCACATCTTC; ACCAACCTTATCCTGGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCTGCAGAGGACCCCATCCGTAGCC; ACTCATTCCGCAACACGATACTGGGCTACTTTGACTATGCCTTCACAGCCATCTTTACGGTTGAAATCCT; GTTAAAGATGACAACTTTTGGAGCCTTCTTGCACAAAGGGGCTTTCTGCAGGAACTACTTCAATTTGCTG; GACATGCTGGTTGTTGGGGTGTCGCTGGTGTCCTTTGGGATTCAGTCCAGCGCCATCTCCGTTGTGAAGA; TTCTGAGGGTTTTGAGGGTCTTGCGGCCTCTCAGAGCAATCAACAGAGCAAAGGGACTTAAGCACGTGGT; CCAGTGTGTCTTTGTGGCCATCCGAACCATTGGCAACATCATGATCGTCACGACCCTGCTCCAGTTCATG; TTTGCATGCATTGGGGTCCAGCTGTTCAAGGGGAAGTTCTACCGTTGCACAGATGAAGCCAAAAGTAACC; CTGAGGAGTGCAGGGGGCTTTTCATTCTTTATAAGGACGGCGATGTTGACAGTCCTGTGGTCCGTGAGAG; GATCTGGCAAAATAGTGATTTCAATTTTGACAATGTCCTTTCGGCTATGATGGCGCTGTTCACAGTCTCA; ACTTTTGAGGGCTGGCCCGCGTTGCTGTACAAAGCTATTGATTCAAACGGTGAGAACGTTGGTCCTGTCT; ACAACTACCGTGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATCGTTGCCTTCTTCATGATGAA; CATCTTTGTGGGCTTCGTCATCGTCACCTTCCAGGAACAGGGAGAAAAAGAGTATAAGAACTGTGAGCTG; GACAAAAATCAGCGTCAGTGTGTTGAATATGCCTTGAAAGCACGCCCCTTAAGGAGATACATCCCCAAAA; ACCCATACCAGTACAAGTTCTGGTATGTGGTGAACTCCTCGCCTTTCGAATACATGATGTTCGTCCTCAT; CATGCTCAACACGCTCTGCCTGGCCATGCAGCACTATGAGCAGTCCAAGATGTTCAATGATGCCATGGAC; ATTCTGAACATGGTCTTCACAGGGGTCTTCACTGTTGAGATGGTTTTGAAAGTCATTGCTTTCAAGCCCA; AGGGGTATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACGTGGC; CCTCAGCGAAGCCGACAACTCTGAAGAGAGCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTG; ATGCGGTTGGTGAAACTTCTCAGCAGGGGGGAAGGCATCCGGACACTGCTGTGGACCTTCATCAAGTCCT; TTCAGGCGCTGCCGTATGTTGCCCTCCTCATTGCCATGCTCTTCTTCATCTACGCTGTCATAGGCATGCA; GATGTTTGGGAAAGTTGCCATGAGAGATAACAACCAGATCAATAGGAACAACAACTTCCAGACCTTTCCT; CAGGCAGTGCTCCTACTCTTCAGGTGTGCAACAGGGGAAGCCTGGCAGGAGATCATGCTTGCCTGCCTCC; CAGGAAAGCTGTGCGACCCAGACTCCGATTACAACCCAGGAGAGGAGTACACTTGTGGGAGCAACTTTGC; CATTGTCTACTTCATCAGCTTTTACATGCTCTGTGCATTCCTGATCATCAACCTCTTTGTGGCTGTCATC; ATGGACAATTTTGACTATCTGACTCGGGACTGGTCTATTCTGGGGCCTCACCACTTGGACGAATTCAAAA; GAATATGGTCAGAATATGACCCTGAAGCAAAGGGAAGGATAAAACACCTTGATGTGGTCACTCTGCTCCG; GCGGATCCAGCCTCCCCTGGGGTTTGGAAAATTATGCCCACACCGAGTAGCATGTAAGAGATTGGTTGCC; ATGAACATGCCTCTCAACAGTGATGGGACAGTCATGTTCAATGCAACCCTGTTTGCTTTGGTCCGGACAG; CTCTCAAGATCAAGACTGAAGGGAACCTGGAGCAAGCTAATGAAGAACTCCGAGCTGTGATCAAGAAAAT; CTGGAAGAAGACAAGCATGAAGCTGCTTGACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTG; GGGAAGTTCTATGCCACTTTCCTGATACAGGACTACTTTAGGAAATTCAAGAAACGGAAAGAGCAAGGAC; TGGTGGGGAAGTACCCTGCGAAGAACACCACGATTGCCCTACAGGCGGGATTAAGGACCCTGCATGACAT; TGGGCCAGAAATCCGACGGGCTATATCCTGTGACTTGCAAGATGACGAGCCAGAAGACTCCAAACCAGAA; GAAGAAGATGTATTCAAAAGAAATGGTGCCCTGCTTGGAAACCATGTCAATCATGTTAATAGTGATAGGA; GAGATTCCCTTCAGCAGACCAATACCACCCACCGTCCCCTGCATGTCCAAAGGCCTTCAATGCCACCTGC; AAGTGATACTGAGAAACCGCTGTTTCCTCCAGCAGGAAATTCGGGGTGTCATAACCATCATAACCATAAT; TCCATAGGGAAGCAAGCTCCCACCTCAACAAATGCCAATCTCAATAATGCCAATATGTCCAAAGCTGCCC; ACGGAAAGCCGCCCAGCATTGGGAACCTTGAGCATGTGTCTGAAAATGGGCATTACTCCTGCAAGCATGA; TCGAGAGCTTCAAAGAAGGTCCAGTATTAAAAGAACCCGCTATTACGAGACTTACATTAGGTCTGAGTCA; GGAGATGAGCAATTTCCAACCATTTGCCGTGAAGATCCTGAGATACACGGCTACTTCCGAGACCCCCGCT; GCTTGGGGGAACAGGAGTATTTCAGTAGCGAGGAGTGCTGTGAGGACGACAGCTCTCCCACCTGGAGCAG; GCAAAACTACAACTACTACAACAGGTACCCAGGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTACCAC; CACCCCCAAGGCTTCTTAGAAGATGATGACTCTCCCACTGGCTATGATTCACGGAGATCTCCGAGGAGAC; GTCTTCTACCTCCTACCCCACCATCCCATCGGAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGCAAAG; CAGCCAAGACGATGTCCTTCCATCGCCTGCCCTGCCTCACCGCGCTGCCCTGCCCCTGCACCTGATGCAG; CAGCAGATCATGGCCGTTGCAGGCCTAGATTCCAGTAAAGCCCAGAAGTACTCACCGAGCCACTCTACCC; GGTCGTGGGCCACCCCTCCAGCAACCCCTCCATACCGAGACTGGAGCCCATGCTACACCCCTTTGATCCA; GGTGGACCGCTCAGAATCTATGGACCAGGTCAATGGCAGCCTGCCATCCCTACACCGAAGCTCCTGGTAC; ACAGATGAACCCGACATCTCCTATAGGACTTTCACGCCAGCTAGTCTGACTGTCCCCAGCAGCTTCCGGA; ACAAGAACAGCGACAAGCAGAGGAGTGCAGACAGCCTGGTGGAGGCAGTCCTGATATCCGAAGGCTTGGG; ACGCTACGCGAGGGACCCCAAATTTGTCTCAGCGACAAAACATGAGATTGCCGATGCCTGTGACCTAACC; ATAGACGAGATGGAAAGTGCTGCCAGCACCCTGCTCAATGGCAGTGTGTGTCCTCGAGCCAATGGGGACA; TGGGCCCCATCTCCCACCGCCAGGACTATGAGCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGGAGCC; AGACCCTGGGCGAGAGGAAGAGGACCTGGCAGATGAGATGATTTGCATTACCACCTTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222925 representing NM_028981; Red=Cloning site Green=Tags(s); ; MNLPTFSSDLILIKSVLSQETDARYKGRVVSAVESTEDFSQAFAEANYARGTRLPISGEGPTSQPNSSKQ; TVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRA; CISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLH; PNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQ; VVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTA; NGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVL; NLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPT; SETESVNTENVSGEGETQGCCGTLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTL; TISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELE; LMSPLGVSVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNF; DETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFL; AIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPY; PPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIF; TNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLL; DMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFM; FACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVS; TFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCEL; DKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMD; ILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRV; MRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFP; QAVLLLFRCATGEAWQEIMLACLPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVI; MDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVA; MNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTV; GKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPE; EEDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHN; SIGKQAPTSTNANLNNANMSKAAHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSES; GDEQFPTICREDPEIHGYFRDPRCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYH; HPQGFLEDDDSPTGYDSRRSPRRRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQ; QQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWY; TDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLT; IDEMESAASTLLNGSVCPRANGDMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028981
• ORF Size: 6498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_028981.3, NP_083257.2
• RefSeq Size: 8705 bp
• RefSeq ORF: 6501 bp
• Locus ID: 12289
• UniProt ID: Q99246
• Cytogenetics: 14 18.43 cM
• MW: 245.7 kDa
• Summary: This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]