Cacna1d (NM_028981) Mouse Untagged Clone
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Cacna1d |
| Synonyms | 8430418G19Rik; C79217; Cach3; Cacn; Cacn4; Cacnl1a2; Cav1.3; Cchl; Cchl1; Cchl1a; Cchl1a2; D-LTC; D-LTCC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_028981 |
| Insert Size | 6501 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_028981.3, NP_083257.2 |
| RefSeq Size | 8701 bp |
| RefSeq ORF | 6501 bp |
| Locus ID | 12289 |
| UniProt ID | Q99246 |
| Cytogenetics | 14 18.43 cM |
| Summary | This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Nov 2014 Transcript Variant: This variant (1) differs in the 5' UTR and has multiple coding region differences, compared to variant 3. These differences cause translation initiation at an alternate start codon, compared to variant 3. This results in a distinct N-terminus and shorter protein (isoform 1), compared to isoform 3. |
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Resources
"NM_028981" in other vectors (2)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG222925 | Cacna1d (tGFP-tagged) - Mouse calcium channel voltage-dependent L type alpha 1D subunit (Cacna1d) transcript variant 1, (10ug) | 10 ug |
$1,987.00
|
|
| MR222925 | Cacna1d (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 1 | 10 ug |
$1,787.00
|
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