D130043K22Rik (NM_001081051) Mouse Tagged ORF Clone

SKU
MR218150
D130043K22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik)
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    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$589.00 MSRP $942.00 MSRP $942.00
In Stock*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D130043K22Rik
Synonyms 4930451E12Rik; Kiaa0319
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218150 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTCCCCACCAGGAGTACTGTCTTCATTGCTGCTGCTGGCAGCCATGGCAGGAGGCAGTTCTCAGC
AGTGCTCTGAGGGCAGGACTTACTCCGATGCCATCATTTCACCTAACCCGGAAACCATCAGAATCATGCG
GGTGTCTCAAACCTTCTCCGTGGGAGACTGCACGGCCGCTTGCTGTGACTTGCTCACCTGTGACCTGGCC
TGGTGGTTTGAGGGCAGCTGCTATCTGGTGAAATGCATGCGCTCGGAGAATTGCGAGCCCAGGACCACAG
GCCCCATCCGGTCTTACCTCACTTTCGTGCGCAGACCTGTCCAGAGGCCCGGGCAGCTGCTGGACTATGG
AGACATGATGCTGAGCAGGGGCTCCCCCTCAGGAGCTTGGGGAGACTCCCTTGAGGACCTCAGGAAGGAC
TTGCCCTTTCTTGGCAAAGACGGGGGACCAGAGGAGACCACTGAGTACTCAGATGAATACAAAGACCTGG
AGCGGGGCCTCCTGCAGCCCTCCAACCAGCAAGACCCTAGAGGTAGCGCAGAGTACCCCGACTGGAGCCT
GCTGCCCAGCAACGAGGGAGGTTTCAATGCTACAGCTACAGGAGACAACTCAGCTGCTTCCATGGAGAAG
CTGCAGGATCCCACGCCCCACCCACTGGACCAGGAGCAGCTGCAGGCCCTGAATGAGTCAACTTGGTCCC
CTACACCCGGGCACTCTTCAATAAGCAGTGTGTGGCCTTCCTCTGCGAGCCCATTACCTACAGAGGAGGG
ACTGGAAGGAGAAGAGACTTTACAGCTCCAAGAACAACCGAGCAATAGCTCTGGAAAAGAGGTTCCAATG
CCTTCCCATAATCCTTCCCCTGCCAGCCTGGAGTCTAGCCCAGCCACGACGGAAAAAAACTCCAACTTTA
CAGTCACGCCACGGAGCAGAAAACACAGCACCCCAACTTTTCCTACCAGTACAGTCCTCACTGGGCTAAC
CCCTCCTCCGTGGCCCTTGTCACCTACTGCTTCCAGGACAGTAAAGGCGCTTGCTGTGTCTGCTGGAGAT
AACCTAGTACTAACCTTACCGGACAGAGAAGCAGAACTGAAGGCCTCTGTTGAACCAGCGCCCCCTGCAG
ATACAACTTACTCCTATGAATGGAGTTTAATGAGCCACCCAGTAGACTTCCAAGGTAAAATCAAACAAGA
AAACAAGCCGACTCTTCACCTCTCTCAATTATCTGTGGGACTCTATGCCTTCAGAGTGGCTGTTTCTAGT
GAGAATGCATTTGGAGAAGGCTATGTCAATGTCACGGTTATGCCAGCTGCAAGAGTCAACCAGCCACCTG
TAGCTGTTGTTTCTCCCCAGACACAAGAGCTCAGTGTGCCTTTGACCTCAGCCCTGATCGATGGCAGCCA
AAGTACAGATGATACTGAAATAGTGAGTTACCACTGGGAAGAAGTCGACGGGCCCTTCCTAGGAGAGGAG
TTTCCAGCTGACACCCCAATCTTACGATTGTCGAACCTAGTTCCTGGTAACTACACCTTTAGGTTGACCA
TCACAGACTCGGATGGCGCTACCAACTCCACCACTGCATCCCTCGTCATCCGTGATGCCGTGGACTACCC
ACCCGTTGCCAATGCAGGGCCAAATCAGACCATCACTTTGCCCCAAAACACCATCATTCTGAATGGGAAC
CAGAGCAGTGATGATCACCAGATTGTTCTCTATGAGTGGTTCGCAGGCCCTGGCGGTGAGAGCAAAGAGA
TGGTCATGCAGGGAGCGCAGACCCCATACCTTCACTTGTCTGAGCTGCAGGAAGGAGAGTACACATTTCA
GCTGATGGTGACGGATTCCTCGGGACAGCAATCCACCGCTTTGGTCGCTGTGACTGTCCAGGCTGAGAAC
AACCAGGCTCCGGTGGCCGTGGCAGGCCCCGACAAGGAGCTGGTTTTCCCGGTGCAGAGTGCTACACTGG
ATGGGAGCCGGAGCAGTGACGATCACGGCATTGTCTGCTATCACTGGGAGCACATCAGAGGCCCCAGTGC
AGTGGAGATGGAGAATGTGGACAAAGCCATAGCCACTGTCACCGGCCTTCAGGTGGGTATCTACCACTTC
CGGTTAACCGTGAGAGACCAGCAGGGTCTGAGCAGCACGTCCACCCTCACTGTGGCTGTGAAGAAGGAAA
ATAACAGCCCTCCCAGAGCCCAGGCTGGTGGCAGACATGTTCTTATACTTCCCAATAATTCCATTACTTT
GGATGGTTCAAGGTCTACTGATGACCGAGGAATTGTGTCCTATCTGTGGATCCGGGATGGCCAGAGTCCA
GCTGCCGGAGATGTCATTGGAGGCTCTGACCACAGGGCAGCTCTGCAGCTCACCAATCTGGTGGAAGGCG
TCTACACTTTTCACTTGCTAGTCACTGACAGTCAGGGGGCCTCGGACTCAGACGCGGCCACTGTGGAGGT
GCTGCCAGACCCCAAGAAGGATGGCCTGGTGGAGTTGATCCTGCAGGTTGGTGTTGAGCAGCTGACAGAG
CAGCAGAAGGAGACTCTAGTGAGGCAGCTGGCTGTGCTCCTGAACGTGCTAGACTCAGACGTGAAGGTGT
TGAAGATCCAGGCTCACACGGATGTCAGCACTGTGATTGTATTTTATGTACAGAGTGGATCACCTTTCAA
GGTTCTCAGAGCTGCTGCTGTGGCCCGGAATCTGCACAAGCGGCTTTCCAAGGAGAAGGAGGCTTTCCTG
CTTTTCAAGGTCTTGAGGGTAGACACAGCAGGGTGCCTTCTCAAGTGTTCAGGCCACGGTCACTGTGACC
CCATCACGAAGCGCTGCATCTGCTCCCAGCTGTGGATGGAGAACCTCATACAGCGGTACATGTGGGATGG
AGAGAGCAACTGTGAGTGGAGTGTATTCTATGTGGCAGCTCTGGCATTGACACTAACACTGCTAACTGGA
GCTGTGTCTTGGCTGTGCATCTGCTGCTGCAGGAGACGGAAAAGGACTAAGATCCGGAAGAAAACCAAGT
ATACCATCCTAGACAGCATGGACGAACAGGAAAGAATGGAACTGAGGCCCAAGTACGGTATCAAGCACAG
GAGCACGGAGCACAACTCCAGTCTGATGGTGTCGGAGTCTGAGTTTGAAAGCGACCAGGACACGCTATTC
AGCCGAGAGAGGATGGAGAGAGGAGTCCTGAAGGGTTCCCTGAATGGCTGTGCCAGAAATGGAGTTTCCT
TCGGTTACTACTCAAAGGACAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218150 protein sequence
Red=Cloning site Green=Tags(s)

MVSPPGVLSSLLLLAAMAGGSSQQCSEGRTYSDAIISPNPETIRIMRVSQTFSVGDCTAACCDLLTCDLA
WWFEGSCYLVKCMRSENCEPRTTGPIRSYLTFVRRPVQRPGQLLDYGDMMLSRGSPSGAWGDSLEDLRKD
LPFLGKDGGPEETTEYSDEYKDLERGLLQPSNQQDPRGSAEYPDWSLLPSNEGGFNATATGDNSAASMEK
LQDPTPHPLDQEQLQALNESTWSPTPGHSSISSVWPSSASPLPTEEGLEGEETLQLQEQPSNSSGKEVPM
PSHNPSPASLESSPATTEKNSNFTVTPRSRKHSTPTFPTSTVLTGLTPPPWPLSPTASRTVKALAVSAGD
NLVLTLPDREAELKASVEPAPPADTTYSYEWSLMSHPVDFQGKIKQENKPTLHLSQLSVGLYAFRVAVSS
ENAFGEGYVNVTVMPAARVNQPPVAVVSPQTQELSVPLTSALIDGSQSTDDTEIVSYHWEEVDGPFLGEE
FPADTPILRLSNLVPGNYTFRLTITDSDGATNSTTASLVIRDAVDYPPVANAGPNQTITLPQNTIILNGN
QSSDDHQIVLYEWFAGPGGESKEMVMQGAQTPYLHLSELQEGEYTFQLMVTDSSGQQSTALVAVTVQAEN
NQAPVAVAGPDKELVFPVQSATLDGSRSSDDHGIVCYHWEHIRGPSAVEMENVDKAIATVTGLQVGIYHF
RLTVRDQQGLSSTSTLTVAVKKENNSPPRAQAGGRHVLILPNNSITLDGSRSTDDRGIVSYLWIRDGQSP
AAGDVIGGSDHRAALQLTNLVEGVYTFHLLVTDSQGASDSDAATVEVLPDPKKDGLVELILQVGVEQLTE
QQKETLVRQLAVLLNVLDSDVKVLKIQAHTDVSTVIVFYVQSGSPFKVLRAAAVARNLHKRLSKEKEAFL
LFKVLRVDTAGCLLKCSGHGHCDPITKRCICSQLWMENLIQRYMWDGESNCEWSVFYVAALALTLTLLTG
AVSWLCICCCRRRKRTKIRKKTKYTILDSMDEQERMELRPKYGIKHRSTEHNSSLMVSESEFESDQDTLF
SRERMERGVLKGSLNGCARNGVSFGYYSKDR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001081051
ORF Size 3243 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081051.1, NM_001081051.2, NP_001074520.1
RefSeq Size 4977 bp
RefSeq ORF 3246 bp
Locus ID 210108
UniProt ID Q5SZV5
Cytogenetics 13 A3.1
MW 118 kDa
Summary This gene encodes a transmembrane protein that contains a large extracellular domain with multiple polycystic kidney disease (PKD) domains. The encoded protein may play a role in the development of the cerebral cortex by regulating neuronal migration and cell adhesion. Single nucleotide polymorphisms in a similar gene in human are associated with dyslexia. Alternatively spliced transcript variants have been identifed. [provided by RefSeq, May 2015]
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SKU Description Size Price
MC223491 D130043K22Rik (untagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik), (10ug) 10 ug
$1,036.00
MG218150 D130043K22Rik (tGFP-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik), (10ug) 10 ug
$1,142.00
MR218150L3 Lenti ORF clone of D130043K22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik) 10 ug
$1,242.00
MR218150L4 Lenti ORF clone of D130043K22Rik (mGFP-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik) 10 ug
$1,242.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.