D130043K22Rik (NM_001081051) Mouse Untagged Clone

SKU MC223491

D130043K22Rik (untagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: D130043K22Rik
• Synonyms: 4930451E12Rik; Kiaa0319
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223491 representing NM_001081051; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGTCCCCACCAGGAGTACTGTCTTCATTGCTGCTGCTGGCAGCCATGGCAGGAGGCAGTTCTCAGC; AGTGCTCTGAGGGCAGGACTTACTCCGATGCCATCATTTCACCTAACCCGGAAACCATCAGAATCATGCG; GGTGTCTCAAACCTTCTCCGTGGGAGACTGCACGGCCGCTTGCTGTGACTTGCTCACCTGTGACCTGGCC; TGGTGGTTTGAGGGCAGCTGCTATCTGGTGAAATGCATGCGCTCGGAGAATTGCGAGCCCAGGACCACAG; GCCCCATCCGGTCTTACCTCACTTTCGTGCGCAGACCTGTCCAGAGGCCCGGGCAGCTGCTGGACTATGG; AGACATGATGCTGAGCAGGGGCTCCCCCTCAGGAGCTTGGGGAGACTCCCTTGAGGACCTCAGGAAGGAC; TTGCCCTTTCTTGGCAAAGACGGGGGACCAGAGGAGACCACTGAGTACTCAGATGAATACAAAGACCTGG; AGCGGGGCCTCCTGCAGCCCTCCAACCAGCAAGACCCTAGAGGTAGCGCAGAGTACCCCGACTGGAGCCT; GCTGCCCAGCAACGAGGGAGGTTTCAATGCTACAGCTACAGGAGACAACTCAGCTGCTTCCATGGAGAAG; CTGCAGGATCCCACGCCCCACCCACTGGACCAGGAGCAGCTGCAGGCCCTGAATGAGTCGACTTGGTCCC; CTACACCCGGGCACTCTTCAATAAGCAGTGTGTGGCCTTCCTCTGCGAGCCCATTACCTACAGAGGAGGG; ACTGGAAGGAGAAGAGACTTTACAGCTCCAAGAACAACCGAGCAATAGCTCTGGAAAAGAGGTTCCAATG; CCTTCCCATAATCCTTCCCCTGCCAGCCTGGAGTCTAGCCCAGCCACGACGGAAAAAAACTCCAACTTTA; CAGTCACGCCACGGAGCAGAAAACACAGCACCCCAACTTTTCCTACCAGTACAGTCCTCACTGGGCTAAC; CCCTCCTCCGTGGCCCTTGTCACCTACTGCTTCCAGGACAGTAAAGGCGCTTGCTGTGTCTGCTGGAGAT; AACCTAGTACTAACCTTACCGGACAGAGAAGCAGAACTGAAGGCCTCTGTTGAACCAGCGCCCCCTGCAG; ATACAACTTACTCCTATGAATGGAGTTTAATGAGCCACCCAGTAGACTTCCAAGGTAAAATCAAACAAGA; AAACAAGCCGACTCTTCACCTCTCTCAATTATCTGTGGGACTCTATGCCTTCAGAGTGGCTGTTTCTAGT; GAGAATGCATTTGGAGAAGGCTATGTCAATGTCACGGTTATGCCAGCTGCAAGAGTCAACCAGCCACCTG; TAGCTGTTGTTTCTCCCCAGACACAAGAGCTCAGTGTGCCTTTGACCTCAGCCCTGATCGATGGCAGCCA; AAGTACAGATGATACTGAAATAGTGAGTTACCACTGGGAAGAAGTCGACGGGCCCTTCCTAGGAGAGGAG; TTTCCAGCTGACACCCCAATCTTACGATTGTCGAACCTAGTTCCTGGTAACTACACCTTTAGGTTGACCA; TCACAGACTCGGATGGCGCTACCAACTCCACCACTGCATCCCTCGTCATCCGTGATGCCGTGGACTACCC; ACCCGTTGCCAATGCAGGGCCAAATCAGACCATCACTTTGCCCCAAAACACCATCATTCTGAATGGGAAC; CAGAGCAGTGATGATCACCAGATTGTTCTCTATGAGTGGTTCGCAGGCCCTGGCGGTGAGAGCAAAGAGA; TGGTCATGCAGGGAGCGCAGACCCCATACCTTCACTTGTCTGAGCTGCAGGAAGGAGAGTACACATTTCA; GCTGATGGTGACGGATTCCTCGGGACAGCAATCCACCGCTTTGGTCGCTGTGACTGTCCAGGCTGAGAAC; AACCAGGCTCCGGTGGCCGTGGCAGGCCCCGACAAGGAGCTGGTTTTCCCGGTGCAGAGTGCTACACTGG; ATGGGAGCCGGAGCAGTGACGATCACGGCATTGTCTGCTATCACTGGGAGCACATCAGAGGCCCCAGTGC; AGTGGAGATGGAGAATGTGGACAAAGCCATAGCCACTGTCACCGGCCTTCAGGTGGGTATCTACCACTTC; CGGTTAACCGTGAGAGACCAGCAGGGTCTGAGCAGCACGTCCACCCTCACTGTGGCTGTGAAGAAGGAAA; ATAACAGCCCTCCCAGAGCCCAGGCTGGTGGCAGACATGTTCTTATACTTCCCAATAATTCCATTACTTT; GGATGGTTCAAGGTCTACTGATGACCGAGGAATTGTGTCCTATCTGTGGATCCGGGATGGCCAGAGTCCA; GCTGCCGGAGATGTCATTGGAGGCTCTGACCACAGGGCAGCTCTGCAGCTCACCAATCTGGTGGAAGGCG; TCTACACTTTTCACTTGCTAGTCACTGACAGTCAGGGGGCCTCGGACTCAGACGCGGCCACTGTGGAGGT; GCTGCCAGACCCCAAGAAGGATGGCCTGGTGGAGTTGATCCTGCAGGTTGGTGTTGAGCAGCTGACAGAG; CAGCAGAAGGAGACTCTAGTGAGGCAGCTGGCTGTGCTCCTGAACGTGCTAGACTCAGACGTGAAGGTGT; TGAAGATCCAGGCTCACACGGATGTCAGCACTGTGATTGTATTTTATGTACAGAGTGGATCACCTTTCAA; GGTTCTCAGAGCTGCTGCTGTGGCCCGGAATCTGCACAAGCGGCTTTCCAAGGAGAAGGAGGCTTTCCTG; CTTTTCAAGGTCTTGAGGGTAGACACAGCAGGGTGCCTTCTCAAGTGTTCAGGCCACGGTCACTGTGACC; CCATCACGAAGCGCTGCATCTGCTCCCAGCTGTGGATGGAGAACCTCATACAGCGGTACATGTGGGATGG; AGAGAGCAACTGTGAGTGGAGTGTATTCTATGTGGCAGCTCTGGCATTGACACTAACACTGCTAACTGGA; GCTGTGTCTTGGCTGTGCATCTGCTGCTGCAGGAGACGGAAAAGGACTAAGATCCGGAAGAAAACCAAGT; ATACCATCCTAGACAGCATGGACGAACAGGAAAGAATGGAACTGAGGCCCAAGTACGGTATCAAGCACAG; GAGCACGGAGCACAACTCCAGTCTGATGGTGTCGGAGTCTGAGTTTGAAAGCGACCAGGACACGCTATTC; AGCCGAGAGAGGATGGAGAGAGGAGTCCTGAAGGGTTCCCTGAATGGCTGTGCCAGAAATGGAGTTTCCT; TCGGTTACTACTCAAAGGACAGATAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081051
• Insert Size: 3246 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001081051.2, NP_001074520.1
• RefSeq Size: 5146 bp
• RefSeq ORF: 3246 bp
• Locus ID: 210108
• UniProt ID: Q5SZV5
• Cytogenetics: 13 A3.1
• Summary: This gene encodes a transmembrane protein that contains a large extracellular domain with multiple polycystic kidney disease (PKD) domains. The encoded protein may play a role in the development of the cerebral cortex by regulating neuronal migration and cell adhesion. Single nucleotide polymorphisms in a similar gene in human are associated with dyslexia. Alternatively spliced transcript variants have been identifed. [provided by RefSeq, May 2015]; Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.