Spata18 (NM_178387) Mouse Tagged ORF Clone

SKU MR213524

Spata18 (Myc-DDK-tagged) - Mouse spermatogenesis associated 18 (Spata18)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Spata18
• Synonyms: 1700067I02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213524 representing NM_178387; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGAAAGCCTGAAGAAGCTGGCCAAAAGCGAATCTTTACAAGCACTGCAGGATAAAGTCACCTACT; GGGTGAACGACTACAACTCAAACTCCTGTGATCAAAATCTAAATTATTGCATCGAACTCATCGAACAAGT; TGCTAAAGTGCAGGCACAGCTCTTTGGGATCCTCACAGTGACAGCCCAAGAAGGGGGAAATAATGAAGGC; GTGGAGACAATCAAGTGTCGCCTTTTGCCCTTGCTGCAGACTTCCTTCAGTTCTGTAAACATGGGGAAAA; CGGCTGAGTCTGAGATGTGTGCTACACAGGATTTCCAACTTAGAAGTAAAAACAGAGACAATTCTCCGGA; TCAGGACCAACATCAATCAGACAATGAATCGTTCTCAGAGACTCAACCAACCCAGGTTCAAGACGATCTG; GCTGAAAGTGGAAAGAGTCTTGAAGGAGCCAAGAACGGATCAACCATATCCCTCCTGGCTGCAGAGGAGG; AGATAAACCAGCTAAAAAAGCAGCTCAAATCTCTCCAAGCTCAGGAGGACGCCCGCCACAAAACCTCGGA; GAACCGTCGCTCTGAGGCCTTAAAAAGTGATCATCGATCAACCAAACGAACCCAGGACCAACGGCCCCAA; GACGTGGTGTCCAACTATGAGAAACATCTGCAAAATCTGAAGGAAGAGATAGCTGTCCTGTCTGCTGAGA; AAAGTGGTCTCCAGGGGAGGTCTGCCAGGAGCCCATCTCCTAGCACAGGCACCCGCAGCCACCGTCGCGG; CCGCAGCCGTAGCCACAGCCGCAGCCGCAGCCACAGCCGGTCCAATAGCCCCTGCACCACAGTGGCCAAG; ATCAGAAGCCCGTCCCCAAACCGTGCCAAAATGTCCAGCGTGGCTCGCAAAGCTGCTCTCCTGTCCCGAT; TCAGCGACGCCTATTCCCAGGCCCGTCTGGATGCTCAGTGCTTGCTGAGGCGTTGCATCGACAGGGCAGA; GACGGTGCAGCGGATCATTTACATCGCCACAGTGGAGGCATTTCATGTAGCTAAAATGGCATTCAGGCAT; TTCAAGATCCGGGTGAGGAAGATGCTGACGCCATCGAACGTGGGATCTAATACTGACTTTGAGACCGCAG; TCTCCGAATACATTGTTTGCCACCTGGACCTGTATGACTCGCAAAGCAGTGTCAATGATGTGATCCGTGC; CATGAACGTCAACCCCAAGATCTCATTCCCTCCTGAAGTTGACTTCTGCCTCCTCACTGACTTCATCCAG; GAGATTTGCTGCATTGCTTTTGCAATGCAGTCCTTAGAGCCACCCCTCGATATCGCATTCGGGGCAGATG; GGGAAATCTTTAACGATTGCAAGTACCGCCGCAGCTATGACTCGGACTTTACTGCTCCCTTGGTCTTTTA; TCACGTGTGGCCTGCTCTCATGGAGAATGACTGTGTCATTATGAAGGGAGAAGCTGTCACCAAAAGAGGG; GCTTTTTGGAGTTCCGTGCGACCAGTAATGCGGTGCCGTAGCAGGAGCTTAAGCCCCATTTGTCCTCGTA; ATCATTTTGGAATAAGCACGGTATCTCGAAGTCGAAGCCCTTCTCCAATAAGATGTACATTCGCAAGGTA; C; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213524 representing NM_178387; Red=Cloning site Green=Tags(s); ; MAESLKKLAKSESLQALQDKVTYWVNDYNSNSCDQNLNYCIELIEQVAKVQAQLFGILTVTAQEGGNNEG; VETIKCRLLPLLQTSFSSVNMGKTAESEMCATQDFQLRSKNRDNSPDQDQHQSDNESFSETQPTQVQDDL; AESGKSLEGAKNGSTISLLAAEEEINQLKKQLKSLQAQEDARHKTSENRRSEALKSDHRSTKRTQDQRPQ; DVVSNYEKHLQNLKEEIAVLSAEKSGLQGRSARSPSPSTGTRSHRRGRSRSHSRSRSHSRSNSPCTTVAK; IRSPSPNRAKMSSVARKAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIYIATVEAFHVAKMAFRH; FKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDLYDSQSSVNDVIRAMNVNPKISFPPEVDFCLLTDFIQ; EICCIAFAMQSLEPPLDIAFGADGEIFNDCKYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTKRG; AFWSSVRPVMRCRSRSLSPICPRNHFGISTVSRSRSPSPIRCTFARY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178387
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178387.3, NP_848474.2
• RefSeq Size: 1949 bp
• RefSeq ORF: 1614 bp
• Locus ID: 73472
• UniProt ID: Q0P557
• Cytogenetics: 5 C3.3
• MW: 60.4 kDa
• Summary: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. May have a role in spermatogenesis, especially in cell differentiation from late elongate spermatids to mature spermatozoa (By similarity). The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity).[UniProtKB/Swiss-Prot Function]