Spata18 (NM_178387) Mouse Untagged Clone

SKU MC218461

Spata18 (untagged) - Mouse spermatogenesis associated 18 (Spata18), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Spata18
• Synonyms: 1700067I02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218461 representing NM_178387; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGAAAGCCTGAAGAAGCTGGCCAAAAGCGAATCTTTACAAGCACTGCAGGATAAAGTCACCTACT; GGGTGAACGACTACAACTCAAACTCCTGTGATCAAAATCTAAATTATTGCATCGAACTCATCGAACAAGT; TGCTAAAGTGCAGGCACAGCTCTTTGGGATCCTCACAGTGACAGCCCAAGAAGGGGGAAATAATGAAGGC; GTGGAGACAATCAAGTGTCGCCTTTTGCCCTTGCTGCAGACTTCCTTCAGTTCTGTAAACATGGGGAAAA; CGGCTGAGTCTGAGATGTGTGCTACACAGGATTTCCAACTTAGAAGTAAAAACAGAGACAATTCTCCGGA; TCAGGACCAACATCAATCAGACAATGAATCGTTCTCAGAGACTCAACCAACCCAGGTTCAAGACGATCTG; GCTGAAAGTGGAAAGAGTCTTGAAGGAGCCAAGAACGGATCAACCATATCCCTCCTGGCTGCAGAGGAGG; AGATAAACCAGCTAAAAAAGCAGCTCAAATCTCTCCAAGCTCAGGAGGACGCCCGCCACAAAACCTCGGA; GAACCGTCGCTCTGAGGCCTTAAAAAGTGATCATCGATCAACCAAACGAACCCAGGACCAACGGCCCCAA; GACGTGGTGTCCAACTATGAGAAACATCTGCAAAATCTGAAGGAAGAGATAGCTGTCCTGTCTGCTGAGA; AAAGTGGTCTCCAGGGGAGGTCTGCCAGGAGCCCATCTCCTAGCACAGGCACCCGCAGCCACCGTCGCGG; CCGCAGCCGTAGCCACAGCCGCAGCCGCAGCCACAGCCGGTCCAATAGCCCCTGCACCACAGTGGCCAAG; ATCAGAAGCCCGTCCCCAAACCGTGCCAAAATGTCCAGCGTGGCTCGCAAAGCTGCTCTCCTGTCCCGAT; TCAGCGACGCCTATTCCCAGGCCCGTCTGGATGCTCAGTGCTTGCTGAGGCGTTGCATCGACAGGGCAGA; GACGGTGCAGCGGATCATTTACATCGCCACAGTGGAGGCATTTCATGTAGCTAAAATGGCATTCAGGCAT; TTCAAGATCCGGGTGAGGAAGATGCTGACGCCATCGAACGTGGGATCTAATACTGACTTTGAGACCGCAG; TCTCCGAATACATTGTTTGCCACCTGGACCTGTATGACTCGCAAAGCAGTGTCAATGATGTGATCCGTGC; CATGAACGTCAACCCCAAGATCTCATTCCCTCCTGAAGTTGACTTCTGCCTCCTCACTGACTTCATCCAG; GAGATTTGCTGCATTGCTTTTGCAATGCAGTCCTTAGAGCCACCCCTCGATATCGCATTCGGGGCAGATG; GGGAAATCTTTAACGATTGCAAGTACCGCCGCAGCTATGACTCGGACTTTACTGCTCCCTTGGTCTTTTA; TCACGTGTGGCCTGCTCTCATGGAGAATGACTGTGTCATTATGAAGGGAGAAGCTGTCACCAAAAGAGGG; GCTTTTTGGAGTTCCGTGCGACCAGTAATGCGGTGCCGTAGCAGGAGCTTAAGCCCCATTTGTCCTCGTA; ATCATTTTGGAATAAGCACGGTATCTCGAAGTCGAAGCCCTTCTCCAATAAGATGTACATTCGCAAGGTA; CTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178387
• Insert Size: 1614 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178387.3, NP_848474.2
• RefSeq Size: 1949 bp
• RefSeq ORF: 1614 bp
• Locus ID: 73472
• UniProt ID: Q0P557
• Cytogenetics: 5 C3.3
• Summary: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. May have a role in spermatogenesis, especially in cell differentiation from late elongate spermatids to mature spermatozoa (By similarity). The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity).[UniProtKB/Swiss-Prot Function]