Cbfa2t2 (NM_009823) Mouse Tagged ORF Clone

SKU MR209247

Cbfa2t2 (Myc-DDK-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (human) (Cbfa2t2), transcript variant 2

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Cbfa2t2
• Synonyms: A430091M07; C330013D05Rik; Cbfa2t2h; MTGR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209247 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGGGCGTCCCTGGAGCGGCCGCCTTCCAACTTGGTTGTGAGAAAAGGGTGCCAGCAATGCCTGGAT; CGCCTGTGGAAGTGAAGATACAGTCAAGATCCTCACCTCCCATCATGCCACCCCTCCCACCAATAAATCC; TGGAGGACCGAGGCCAGTGTCCTTTACTCCTACAGCATTAAGCAATGGCATCAACCATTCCCCTCCTACC; CTGAATGGTGCCCCATCGCCACCACAGAGATTCAGCAATGGGCCTGCCTCCTCCACATCGTCAGCACTAA; CGAATCAACAGCTGCCGGCTACTTGTGGTGCTCGGCAGCTCAGCAAGTTGAAACGCTTCCTCACCACTCT; GCAGCAGTTTGGCAATGATATCTCACCTGAGATCGGCGAGAAGGTTCGGACTCTCGTTCTAGCCTTGGTG; AACTCAACAGTAACAATTGAAGAATTCCATTGCAAGCTCCAGGAGGCCACAAACTTTCCTCTTCGTCCAT; TTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCGAGAACTGCTGCACTGTGCCCGGGCTGC; CAAGCAGACCCCGTCCCAGTACCTGGCCCAGCATGAGCACCTTCTGCTCAACACCAGTATCGCATCTCCT; GCTGACTCTTCTGAACTGCTCATGGAGGTTCATGGAAATGGAAAGAGGCCCAGTCCGGAAAGGAGGGACG; AGAATAATTTTGAAAGAGATACAGTTCCTCCTGAACCGCCTGCCAAGAGAGTGTGTACTATCAGCCCTGC; TCCTCGGCACAGTCCCGCCCTCACTGTTCCCCTTATGAATCCTGGGGGCCAGTTCCATCCTACTCCACCA; CCTCTTCAGCACTATACACTAGAAGACATTGCAACTTCTCACCTGTATCGGGAACCAAACAAGATGCTAG; AACACCGAGAAGTTCGTGAGAGGCACCACAATCTTAGTCTCAATGGAGGCTATCAAGATGAGTTGGTAGA; CCACCGTTTGACAGAAAGGGAGTGGGCTGATGAATGGAAACACCTTGACCATGCACTGAATTGCATTATG; GAAATGGTAGAGAAAACTAGGCGCTCCATGGCAGTTCTGAGGCGCTGTCAGGAATCTGACCGTGAAGAAC; TCAACTATTGGAAACGGCGGTTTAATGAAAATACAGAGTTGAGGAAAACTGGGACTGAGTTGGTCTCTAG; GCAGCACAGCCCTGGGAGTACAGATTCTCTCAGCAACGATTCTCAGCGGGAATTTACCAGTAGACCAGCA; ACAGGATATGTGCCTGTGGAGTTTTGGAAGAAAACAGAAGAAGCCGTGAATAAGGTGAAAATTCAGGCCA; TGTCAGAGGTACAGAAGGCTGTAGCTGAGGCAGAGCAAAAAGCCTTTGAAGTGATTGCAACGGAGAGAGC; TCGAATGGAGCAGACCATAGCGGATGTCAAACGGCAGGCTGCAGAAGATGCTTTCCTGGTCATCAATGAG; CAAGAAGAGTCCACAGAGAACTGCTGGAACTGTGGCCGAAAAGCCAGTGAGACGTGCAGTGGCTGCAACA; TTGCTCGCTACTGCGGCTCCTTCTGCCAGCATAAGGACTGGGAGCGGCACCACCGCCTCTGTGGCCAGAG; TCTACATGGCCACAGTCCCCACAGCCAGAGCAGGCCACTGCTCCCTGGAGGGCGGGGCTCAGCCAGGTCT; GCTGACTGCAGTGTGCCCAGCCCAGCCCTGGACAAGACCTCAGCTACCACCTCACGGTCCTCAACACCTG; CCTCTGTGACAGCCATTGATGCCAACGGGCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209247 protein sequence; Red=Cloning site Green=Tags(s); ; MVGVPGAAAFQLGCEKRVPAMPGSPVEVKIQSRSSPPIMPPLPPINPGGPRPVSFTPTALSNGINHSPPT; LNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEKVRTLVLALV; NSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHLLLNTSIASP; ADSSELLMEVHGNGKRPSPERRDENNFERDTVPPEPPAKRVCTISPAPRHSPALTVPLMNPGGQFHPTPP; PLQHYTLEDIATSHLYREPNKMLEHREVRERHHNLSLNGGYQDELVDHRLTEREWADEWKHLDHALNCIM; EMVEKTRRSMAVLRRCQESDREELNYWKRRFNENTELRKTGTELVSRQHSPGSTDSLSNDSQREFTSRPA; TGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAAEDAFLVINE; QEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQSLHGHSPHSQSRPLLPGGRGSARS; ADCSVPSPALDKTSATTSRSSTPASVTAIDANGL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009823
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_009823.1, NP_033953.1
• RefSeq Size: 6097 bp
• RefSeq ORF: 1782 bp
• Locus ID: 12396
• UniProt ID: O70374
• Cytogenetics: 2 H1
• MW: 65.9 kDa
• Summary: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency. Involved in primordial germ cell (PCG) formation (PubMed:27281218). Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent mannerCan repress the expression of MMP7 in a ZBTB33-dependent manner (By similarity). Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:19799863). Required for the maintenance of the secretory cell lineage in the small intestine (PubMed:16227606). Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (PubMed:25398765).[UniProtKB/Swiss-Prot Function]