HER2| Breast Cancer |Gastric Cancer | Lung Cancer
HER2 gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It is found in 10-30% of all breast cancers, 29% of gastric cancers, 10% of lung cancers, and 18-61% of uterine cancers.
- 10-30% of all breast cancers
- 29% of gastric cancers
- 10% of lung cancers
- 18-61% of uterine cancers
The diagram depicts HER2 amplification at the protein and DNA level.
HER2 UltraMab™ Antibody
| SKU# | & |
|---|---|
| Clone Name | UMAB36 |
| Target | Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1 |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat, Monkey |
| Tested Application | IHC, IF, WB |
| Dilution | WB: 1:500, IHC: 1:100, IF: 1:100 |
The four panels show different levels of HER2 expression in FFPE human breast cancer. IHC was done on a breast cancer tissue microarray using mouse anti-HER2 clone UMAB36 (SKU# UM500036) monoclonal antibody (1:100). Anti-HER2 clone UMAB36 requires HIER pretreatment with Tris/EDTA pH 8.5 buffer for optimal staining. HER2 expression levels were evaluated by a board certified pathologist.
Immunofluorescence image of FFPE HER2+ breast cancer labeled with HER2 mouse monoclonal antibody clone UMAB36 (red, SKU# UM500036) and DAPI (blue) for nuclear staining.
Western blot analysis of HepG2, HeLa, A549, and MCF7 cell lines extracts (35ug per lane) using anti-HER2 antibody (clone UMAB36) at 1:500.
| Protein Name | Antibody Description | SKU |
|---|---|---|
| CD16 alpha | Rabbit Polyclonal Anti-FCGR3A (CD16 alpha) Antibody | |
| CTNNB1 | Beta-Catenin (CTNNB1) mouse monoclonal antibody, clone UMAB15 | |
| EGF | Anti-EGF Rabbit Polyclonal Antibody | |
| EGFR | EGFR mouse monoclonal antibody,clone UMAB95 | |
| EGFR4/HER4 | Rabbit Polyclonal Anti-EGFR4/HER4/ERBB4 Antibody | |
| ER (ESR1) | ESR1 (Estrogen Receptor 1/ER) mouse monoclonal antibody, clone OTI1B1 | |
| GRB2 | Rabbit Polyclonal Anti-GRB2 Antibody | |
| HB-EGF | Anti HBEGF (Center) polyclonal antibody | |
| HSP90AA1 | Rabbit polyclonal anti-HSP90AA1(HSP90) antibody, Loading control | |
| Ki67 | Purified KI67 (MKI67) mouse monoclonal antibody,clone UMAB107 | |
| LRG1 | Rabbit polyclonal anti-LRG1 (A2GL) antibody | |
| p38 MAPK | Rabbit Anti-p38 MAPK (Thr180/Tyr182) Antibody (Phospho-Specific) | |
| MUC1 | MUC1/EMA mouse monoclonal antibody, clone UMAB57 | |
| NGR1 | Rabbit Polyclonal Anti-Nogo (NGR1) Receptor | |
| p53 | Purified P53 (TP53) mouse monoclonal antibody, clone UMAB62 | |
| Phospholipase C | Anti-PHOSPHOLIPASE C / PLC polyclonal antibody | |
| PICK1 | Rabbit Polyclonal Anti-PICK1 Antibody | |
| PICK3R5 | PIK3R5 mouse monoclonal antibody, clone OTI5B1 | |
| PLCG1 | Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) Mouse Monoclonal Antibody | |
| PR | PR (PgR) mouse monoclonal antibody,clone UMAB136 | |
| SHC1 | Anti-SHC1 (SHC) mouse monoclonal antibody, clone OTI3A1 | |
| SRC | Rabbit Polyclonal Src (Tyr529) Antibody (Phospho-specific) | |
| TGFalpha | Anti-TGFA Rabbit Polyclonal Antibody | |
| cd16alpha | Rabbit Polyclonal Anti-FCGR3A (CD16 alpha) Antibody |