HER2 gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It is found in 10-30% of all breast cancers, 29% of gastric cancers, 10% of lung cancers, and 18-61% of uterine cancers.
- 10-30% of all breast cancers
- 29% of gastric cancers
- 10% of lung cancers
- 18-61% of uterine cancers
The diagram depicts HER2 amplification at the protein and DNA level.
HER2 UltraMab™ Antibody
|SKU#||UM500036 (100ul) & UM570036 (30ul)|
|Target||Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 1|
|Reactivity||Human, Mouse, Rat, Monkey|
|Tested Application||IHC, IF, WB|
|Dilution||WB: 1:500, IHC: 1:100, IF: 1:100|
The four panels show different levels of HER2 expression in FFPE human breast cancer. IHC was done on a breast cancer tissue microarray using mouse anti-HER2 clone UMAB36 (SKU# UM500036) monoclonal antibody (1:100). Anti-HER2 clone UMAB36 requires HIER pretreatment with Tris/EDTA pH 8.5 buffer for optimal staining. HER2 expression levels were evaluated by a board certified pathologist.
Immunofluorescence image of FFPE HER2+ breast cancer labeled with HER2 mouse monoclonal antibody clone UMAB36 (red, SKU# UM500036) and DAPI (blue) for nuclear staining.
Western blot analysis of HepG2, HeLa, A549, and MCF7 cell lines extracts (35ug per lane) using anti-HER2 antibody (clone UMAB36) at 1:500.
|Protein Name||Antibody Description||SKU|
|CD16 alpha||Rabbit Polyclonal Anti-FCGR3A (CD16 alpha) Antibody||TA350080|
|CTNNB1||Beta-Catenin (CTNNB1) mouse monoclonal antibody, clone UMAB15||UM500015|
|EGF||Anti-EGF Rabbit Polyclonal Antibody||TA324063|
|EGFR||EGFR mouse monoclonal antibody,clone UMAB95||UM500070|
|EGFR4/HER4||Rabbit Polyclonal Anti-EGFR4/HER4/ERBB4 Antibody||TA349275|
|ER (ESR1)||ESR1 (Estrogen Receptor 1/ER) mouse monoclonal antibody, clone OTI1B1||TA503759|
|GRB2||Rabbit Polyclonal Anti-GRB2 Antibody||TA349332|
|HB-EGF||Anti HBEGF (Center) polyclonal antibody||AP52008PU-N|
|HSP90AA1||Rabbit polyclonal anti-HSP90AA1(HSP90) antibody, Loading control||TA332385|
|Ki67||Purified KI67 (MKI67) mouse monoclonal antibody,clone UMAB107||UM800033|
|LRG1||Rabbit polyclonal anti-LRG1 (A2GL) antibody||TA316028|
|p38 MAPK||Rabbit Anti-p38 MAPK (Thr180/Tyr182) Antibody (Phospho-Specific)||TA309206|
|MUC1||MUC1/EMA mouse monoclonal antibody, clone UMAB57||UM800008|
|NGR1||Rabbit Polyclonal Anti-Nogo (NGR1) Receptor||TA328650|
|p53||Purified P53 (TP53) mouse monoclonal antibody, clone UMAB62||UM500053|
|Phospholipase C||Anti-PHOSPHOLIPASE C / PLC polyclonal antibody||AP21376AF-N|
|PICK1||Rabbit Polyclonal Anti-PICK1 Antibody||TA350769|
|PICK3R5||PIK3R5 mouse monoclonal antibody, clone OTI5B1||TA505815|
|PLCG1||Anti-PLCG1 (phospho-Tyr771) Rabbit Polyclonal Antibody||TA322973|
|PR||PR (PgR) mouse monoclonal antibody,clone UMAB136||UM800037|
|SHC1||Anti-SHC1 (SHC) mouse monoclonal antibody, clone OTI3A1||TA501056|
|SRC||Rabbit Polyclonal Src (Tyr529) Antibody (Phospho-specific)||TA325880|
|TGFalpha||Anti-TGFA Rabbit Polyclonal Antibody||TA323258|
|cd16alpha||Rabbit Polyclonal Anti-FCGR3A (CD16 alpha) Antibody||TA350080|