1. Q: What is loading control?
A: Loading control proteins are known to be constitutively and stably expressed at high levels in almost all tissues and cells. Housekeeping proteins, such as GAPDH, beta-tubulin and beta-actin represent some of the well-known loading control proteins.
2. Q: What's the main purpose of loading control antibody?
A: Loading control antibodies help determine whether samples have been loaded equally in each lane. This information is needed to determine whether the difference in levels of the protein of interest in different samples is due to loading variance or sample-to-sample expression level differences. Loading control antibodies also help to serve to display the effective transfer of protein during western blotting.
3. Q: What should be considered when choosing a loading control antibody?
4. Q: What’s the advantage of OriGene loading control antibodies?