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Lenti ORF particles, Lef1 (Myc-DDK-tagged) - Mouse lymphoid enhancer binding factor 1 (cDNA clone MGC:66504 IMAGE:6401514), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Lef1 (GFP-tagged) - Mouse lymphoid enhancer binding factor 1 (cDNA clone MGC:66504 IMAGE:6401514), 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Lef1 (Myc-DDK-tagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Lef1 (GFP-tagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lef1 CRISPRa kit - CRISPR gene activation of mouse lymphoid enhancer binding factor 1

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Lef1 (untagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), (10ug)

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

qSTAR qPCR primer pairs against Mus musculus gene Lef1

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.

Lef1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin

Lef1 (untagged) - Mouse lymphoid enhancer binding factor 1 (cDNA clone MGC:66504 IMAGE:6401514), (10ug)

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

Lef1 (untagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), transcript variant 3

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

Lef1 (untagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), transcript variant 2

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

AAV ORF Particles, serotype AAV-2, Lef1 (myc-DDK-tagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), transcript variant 2, 250ul, >10^13 GC/mL

  • AAV ORF®

Lef1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Lef1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

Lef1 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti