pGFP-C-shLenti shRNA Vector

Name: pGFP-C-shLenti shRNA Vector
SKU: TR30023

CAT#: TR30023

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HuSH shRNA GFP Lenti Cloning Vector (pGFP-C-shLenti Vector), 5 ug

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Citations (23)

USD 430.00

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Product Data
Control Type	Negative
Fluorescent Label	GFP
Viral Packaging	Lentiviral
Feature	pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
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Citations (23)

The use of this Vectors has been cited in the following citations:
Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate ,Wu, CC;Peterson, A;Zinshteyn, B;Regot, S;Green, R;, Cell 2020 ,PubMed ID 32610081 [pGFP-C-shLenti]
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development ,Bikbaev, A;Ciuraszkiewicz-Wojciech, A;Heck, J;Klatt, O;Freund, R;Mitlöhner, J;Enrile Lacalle, S;Sun, M;Repetto, D;Frischknecht, R;Ablinger, C;Rohlmann, A;Missler, M;Obermair, GJ;Di Biase, V;Heine, M;, J. Neurosci. 2020 ,PubMed ID 32414783 [pGFP-C-shLenti]
Mir-370-3p Impairs Glioblastoma Stem-Like Cell Malignancy Regulating a Complex Interplay between HMGA2/HIF1A and the Oncogenic Long Non-Coding RNA (lncRNA) NEAT1 ,Lulli, V;Buccarelli, M;Ilari, R;Castellani, G;De Dominicis, C;Di Giamberardino, A;D Alessandris, QG;Giannetti, S;Martini, M;Stumpo, V;Boe, A;De Luca, G;Biffoni, M;Marziali, G;Pallini, R;Ricci-Vitiani, L;, Int J Mol Sci 2020 ,PubMed ID 32443824 [pGFP-C-shLenti]
LINC02476 Promotes the Malignant Phenotype of Hepatocellular Carcinoma by Sponging miR-497 and Increasing HMGA2 Expression ,Duan, Y;Zhao, M;Jiang, M;Li, Z;Ni, C;, Onco Targets Ther 2020 ,PubMed ID 32280244 [pGFP-C-shLenti ]
Casticin elicits inflammasome-induced pyroptosis through activating PKR/JNK/NF-κB signal in 5-8F cells ,Jiang, C;Shi, R;Chen, B;Yan, X;Tang, G;, Biomed. Pharmacother. 2020 ,PubMed ID 31951974 [pGFP-C-shLenti]
Alpha-Catulin, a New Player in a Rho Dependent Apical Constriction That Contributes to the Mouse Neural Tube Closure ,Karpińska, K;Cao, C;Yamamoto, V;Gielata, M;Kobielak, A;, Front. Cell Dev. Biol. 2020 ,PubMed ID 32258033 [pGFP-C-shLenti]
Identification of a LIF-Responsive, Replication-Competent Subpopulation of Human β Cells ,Rosado-Olivieri, EA;Aigha, II;Kenty, JH;Melton, DA;, Cell Metab. 2020 ,PubMed ID 31928884 [pGFP-C-shLenti]
Endocytosis of very low-density lipoproteins: an unexpected mechanism for lipid acquisition by breast cancer cells ,Lupien, LE;Bloch, K;Dehairs, J;Traphagen, NA;Feng, WW;Dennis, T;Davis, WL;Swinnen, JV;Wells, WA;Smits, NC;Kuemmerle, NB;Miller, TW;Kinlaw, WB;, J. Lipid Res. 2019 ,PubMed ID 31806729 [pGFP-C-shLenti]
BC200 overexpression contributes to luminal and triple negative breast cancer pathogenesis ,Barton, M;Santucci-Pereira, J;Vaccaro, OG;Nguyen, T;Su, Y;Russo, J;, BMC Cancer 2019 ,PubMed ID 31646972 [pGFP-C-shLenti]
Argonaute 4 as an Effector Protein in RNA-Directed DNA Methylation in Human Cells ,Chalertpet, K;Pin-On, P;Aporntewan, C;Patchsung, M;Ingrungruanglert, P;Israsena, N;Mutirangura, A;, Front Genet 2019 ,PubMed ID 31333722 [pGFP-C-shLenti]
Melatonin protects chronic kidney disease mesenchymal stem cells against senescence via PrPC dependent enhancement of the mitochondrial function ,Han, YS;Kim, S;Lee, JH;Jung, SK;Noh, H;Lee, SH;, J. Pineal Res. 2018 ,PubMed ID 30372554 [pGFP-C-shLenti]
A conserved HH-Gli1-Mycn network regulates heart regeneration from newt to human ,Singh, BN;Koyano-Nakagawa, N;Gong, W;Moskowitz, IP;Weaver, CV;Braunlin, E;Das, S;van Berlo, JH;Garry, MG;Garry, DJ;, Nat Commun 2018 ,PubMed ID 30315164 [pGFP-C-shLenti]
LINC01287 regulates tumorigenesis and invasion via miR-298/MYB in hepatocellular carcinoma ,Mo, Y;He, L;Lai, Z;Wan, Z;Chen, Q;Pan, S;Li, L;Li, D;Huang, J;Xue, F;Che, S;, J. Cell. Mol. Med. 2018 ,PubMed ID 30133116 [pGFP-C-shLenti]
LINC01287/miR-298/STAT3 feedback loop regulates growth and the epithelial-to-mesenchymal transition phenotype in hepatocellular carcinoma cells ,Mo, Y;He, L;Lai, Z;Wan, Z;Chen, Q;Pan, S;Li, L;Li, D;Huang, J;Xue, F;Che, S;, J. Exp. Clin. Cancer Res. 2018 ,PubMed ID 30001751 [pGFP-C-shLenti]
miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells ,Jia, Z;Zhang, Y;Xu, Q;Guo, W;Guo, A;, Oncol Lett 2018 ,PubMed ID 29725450 [pGFP-C-shLenti]
Interleukin-30/IL-27p28 shapes prostate cancer stem-like cell behavior and is critical for tumor onset and metastasization ,Sorrentino, C;Ciummo, SL;Cipollone, G;Caputo, S;Bellone, M;Di Carlo, E;, Cancer Res. 2018 ,PubMed ID 29487200 [pGFP-C-shLenti]
Global metabolic profiling identifies a pivotal role of proline and hydroxyproline metabolism in supporting hypoxic response in hepatocellular carcinoma ,Tang, L;Zeng, J;Geng, P;Fang, C;Wang, Y;Sun, M;Wang, C;Wang, J;Yin, P;Hu, C;Guo, L;Yu, JJ;Gao, P;Li, E;Zhuang, Z;Xu, G;Liu, Y;, Clin. Cancer Res.2017 ,PubMed ID 29084919 [pGFP-C-shLenti]
Monocrotaline Induces Endothelial Injury and Pulmonary Hypertension by Targeting the Extracellular Calcium-Sensing Receptor ,Xiao, R;Su, Y;Feng, T;Sun, M;Liu, B;Zhang, J;Lu, Y;Li, J;Wang, T;Zhu, L;Hu, Q;, J Am Heart Assoc 2017 ,PubMed ID 28330842 [pGFP-C-shLenti]
PNPLA1 is a transacylase essential for the generation of the skin barrier lipid ω-O-acylceramide ,Ohno, Y;Kamiyama, N;Nakamichi, S;Kihara, A;, Nat Commun 2017 ,PubMed ID 28248318 [pGFP-C-shLenti]
High Expression of Cell Division Cycle 42 Promotes Pancreatic Cancer Growth and Predicts Poor Outcome of Pancreatic Cancer Patients ,Yang, D;Zhang, Y;Cheng, Y;Hong, L;Wang, C;Wei, Z;Cai, Q;Yan, R;, Dig. Dis. Sci 2017 ,PubMed ID 28181096 [pGFP-C-shLenti]
Targeting tissue inhibitor of metalloproteinase 1/2 using a shRNA lentiviral system offers a novel treatment strategy against hepatic fibrosis ,Zhang, S;Liu, Q;Xiao, J;Lei, J;Chai, Y;Liu, Y;Hong, Z;, Int J Clin Exp Med 2016 [pGFP-C-shLenti]
Mutual inhibition of insulin signaling and PHLPP-1 determines cardioprotective efficiency of Akt in aged heart ,Xing, Y;Sun, W;Wang, Y;Gao, F;Ma, H;, Aging (Albany NY) Mar 2016 ,PubMed ID 27019292
Protein composition and movements of membrane swellings associated with primary cilia ,Mohieldin, AM;Haymour, HS;Lo, ST;AbouAlaiwi, WA;Atkinson, KF;Ward, CJ;Gao, M;Wessely, O;Nauli, SM;, Cell. Mol. Life Sci. Feb 2015 ,PubMed ID 25650235 [TR30023]

Documents

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Hush shRNA Cloning Vectorsr
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FAQ for shRNA Vetors

Resources

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual

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