pGFP-C-shLenti shRNA Vector

CAT#: TR30023

HuSH shRNA GFP Lenti Cloning Vector (pGFP-C-shLenti Vector), 5 ug

Product Datasheet for 'TR30023'

Cited in 14 publications.

USD 420.00

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Product Data
Control Type	Negative
Fluorescent Label	GFP
Bacterial selection marker	Chloramphenicol
Mammalian selection marker	Puromycin
Viral Packaging	Lentiviral
Feature	pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
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Citations (14)

The use of this Vectors has been cited in the following citations:
Melatonin protects chronic kidney disease mesenchymal stem cells against senescence via PrPC-dependent enhancement of the mitochondrial function ,Han, Y;Kim, S;Lee, J;Jung, S;Noh, H;Lee, S;, J Pineal Res 2018 ,PubMed ID 30372554 [pGFP-C-shLenti]
Melatonin protects chronic kidney disease mesenchymal stem cells against senescence via PrPC dependent enhancement of the mitochondrial function ,Han, YS;Kim, S;Lee, JH;Jung, SK;Noh, H;Lee, SH;, J. Pineal Res. 2018 ,PubMed ID 30372554 [pGFP-C-shLenti]
A conserved HH-Gli1-Mycn network regulates heart regeneration from newt to human ,Singh, BN;Koyano-Nakagawa, N;Gong, W;Moskowitz, IP;Weaver, CV;Braunlin, E;Das, S;van Berlo, JH;Garry, MG;Garry, DJ;, Nat Commun 2018 ,PubMed ID 30315164 [pGFP-C-shLenti]
LINC01287 regulates tumorigenesis and invasion via miR-298/MYB in hepatocellular carcinoma ,Mo, Y;He, L;Lai, Z;Wan, Z;Chen, Q;Pan, S;Li, L;Li, D;Huang, J;Xue, F;Che, S;, J. Cell. Mol. Med. 2018 ,PubMed ID 30133116 [pGFP-C-shLenti ]
LINC01287/miR-298/STAT3 feedback loop regulates growth and the epithelial-to-mesenchymal transition phenotype in hepatocellular carcinoma cells ,Mo, Y;He, L;Lai, Z;Wan, Z;Chen, Q;Pan, S;Li, L;Li, D;Huang, J;Xue, F;Che, S;, J. Exp. Clin. Cancer Res. 2018 ,PubMed ID 30001751 [pGFP-C-shLenti]
miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells ,Jia, Z;Zhang, Y;Xu, Q;Guo, W;Guo, A;, Oncol Lett 2018 ,PubMed ID 29725450 [pGFP-C-shLenti]
Interleukin-30/IL-27p28 shapes prostate cancer stem-like cell behavior and is critical for tumor onset and metastasization ,Sorrentino, C;Ciummo, SL;Cipollone, G;Caputo, S;Bellone, M;Di Carlo, E;, Cancer Res. 2018 ,PubMed ID 29487200 [pGFP-C-shLenti]
Global metabolic profiling identifies a pivotal role of proline and hydroxyproline metabolism in supporting hypoxic response in hepatocellular carcinoma ,Tang, L;Zeng, J;Geng, P;Fang, C;Wang, Y;Sun, M;Wang, C;Wang, J;Yin, P;Hu, C;Guo, L;Yu, JJ;Gao, P;Li, E;Zhuang, Z;Xu, G;Liu, Y;, Clin. Cancer Res.2017 ,PubMed ID 29084919 [pGFP-C-shLenti]
Monocrotaline Induces Endothelial Injury and Pulmonary Hypertension by Targeting the Extracellular Calcium-Sensing Receptor ,Xiao, R;Su, Y;Feng, T;Sun, M;Liu, B;Zhang, J;Lu, Y;Li, J;Wang, T;Zhu, L;Hu, Q;, J Am Heart Assoc 2017 ,PubMed ID 28330842 [pGFP-C-shLenti]
PNPLA1 is a transacylase essential for the generation of the skin barrier lipid ω-O-acylceramide ,Ohno, Y;Kamiyama, N;Nakamichi, S;Kihara, A;, Nat Commun 2017 ,PubMed ID 28248318 [pGFP-C-shLenti]
High Expression of Cell Division Cycle 42 Promotes Pancreatic Cancer Growth and Predicts Poor Outcome of Pancreatic Cancer Patients ,Yang, D;Zhang, Y;Cheng, Y;Hong, L;Wang, C;Wei, Z;Cai, Q;Yan, R;, Dig. Dis. Sci 2017 ,PubMed ID 28181096 [pGFP-C-shLenti]
Targeting tissue inhibitor of metalloproteinase 1/2 using a shRNA lentiviral system offers a novel treatment strategy against hepatic fibrosis ,Zhang, S;Liu, Q;Xiao, J;Lei, J;Chai, Y;Liu, Y;Hong, Z;, Int J Clin Exp Med 2016 [pGFP-C-shLenti]
Mutual inhibition of insulin signaling and PHLPP-1 determines cardioprotective efficiency of Akt in aged heart ,Xing, Y;Sun, W;Wang, Y;Gao, F;Ma, H;, Aging (Albany NY) Mar 2016 ,PubMed ID 27019292
Protein composition and movements of membrane swellings associated with primary cilia ,Mohieldin, AM;Haymour, HS;Lo, ST;AbouAlaiwi, WA;Atkinson, KF;Ward, CJ;Gao, M;Wessely, O;Nauli, SM;, Cell. Mol. Life Sci. Feb 2015 ,PubMed ID 25650235 [TR30023]

Documents

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Hush shRNA Cloning Vectorsr
FAQs
FAQ for shRNA Vetors
SDS
shRNA Vectors

Resources

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging Manual: CRISPR Vectors

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