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pGFP-C-shLenti shRNA Vector

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SKU
TR30023
HuSH shRNA GFP Lenti Cloning Vector (pGFP-C-shLenti Vector), 5 ug
  $880.00
In Stock*
Specifications
Specifications
Product Data
Control Type Negative
Fluorescent Label TurboGFP
Mammalian Cell Selection Puromycin
Viral Packaging Lentiviral
Features
  • pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.
  • U6_F Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Components 1 vial of 5 ug lyophilized pGFP-C-shLenti shRNA cloning vector
Quality Control This vector was sequence verified and functionally validated.
Storage Store the vector DNA at -20°C.
Stability Vector DNA is stable for at least one year from date of receipt when stored at -20°C.
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Documents
Resources

file-alt Citations

Functional Contribution of Multienzyme Glucosome Condensates to Cellular Redox Homeostasis in Cancer Cells
Sharma, K., Kyoung et al
bioRxiv (2026)
AI Summary: OriGene's pGFP-C-ShLenti vector (Cat. #TR30023) was used to construct shRNA expression plasmids, enabling gene knockdown experiments that were crucial for investigating the role of specific enzymes in glucosome-mediated redox homeostasis in cancer cells.
Snippet: Plasmids expressing short-hairpin RNA (shRNA) were constructed by ligating validated oligonucleotide sequences into the pGFP-C-ShLenti vector (Origene, Cat. #TR30023) using BamHI and BsmBI restriction sites. Briefly, a set of 5’-phosphate oligonucleotide sequences for human catalase (hCAT) [70]
The cytoskeletal regulator Coronin-1A plays a multidirectional role in glioblastoma stemness
Zhang, Shibahara, Hide et al
Sci Rep (2026)
AI Summary: OriGene contributed to this study by providing the pGFP-C-shLenti vector encoding shRNA against human Coro1A (Cat. No. TR30023), which was used to generate Coro1A-knockdown glioblastoma cell clones for functional analyses.
Snippet: The pGFP-C-shLenti vector encoding shRNA against human Coro1A was purchased from Origene Technologies (Rockville, MD, USA;Cat. No. TR30023). The effective shRNA sequences were: #A, 5′- GACACCAACATCGTCTACCTCTGTGGCAA-3′;and
Metabolic Coherence of the Mouse Brain
Liu, Ma, Ribas et al
bioRxiv (2026)
AI Summary: OriGene products, specifically the lentivirus encoding shRNA targeting HIF1α (TL517255V) and the control scrambled shRNA lentivirus (TR30023), were used to achieve targeted gene knockdown in the hippocampus, enabling the study of metabolic and behavioral effects in the mouse brain.
Snippet: For HIF1α knockdown experiments, lentivirus encoding shRNA targeting HIF1α (TL517255V;OriGene) or control scrambled shRNA lentivirus (TR30023;OriGene) was
Targeting the STK39/ARID2 Axis to Inhibit NF-κB Signaling: A Novel Pathway for Mesenchymal Stem Cell Osteogenic Differentiation in Osteoporosis Management
Wang, Wei, Chen et al
J Musculoskelet Neuronal Interact (2026) 26 (1), 124-133 DOI: 10.22540/JMNI-26-124
AI Summary: OriGene products, specifically the negative control siRNA (siNC; TR30023) and negative control plasmid (NC; PS100093), were used as experimental controls in gene silencing and overexpression studies to investigate the roles of ARID2 and STK39 in mesenchymal stem cell differentiation in osteoporosis research.
Snippet: ding negative controls (siNC;TR30023 and NC;PS100093), were obtained from OriGene (Rockville, MD, USA). BMSCs were allocated to the following groups: siNC, siARID2, NC + siNC, NC + siARID2, STK39 overexpression + siNC, and STK39 overexpression + siARID2. For transduction, 1
FigureFigureFigure
APOs as promising prognostic biomarkers: correlation with tumor-infiltrating leukocytes in endometrial cancer
Zhou, Wang, Du et al
Front Immunol (2026) 17, 1646920 DOI: 10.3389/fimmu.2026.1646920
AI Summary: OriGene products, specifically the negative control (TR30023) and transfection reagent (TF81001), were used for transfecting endometrial cancer cells in experiments assessing the functional roles of APO genes in this study.
Snippet: ), and the corresponding control (negative control;TR30023;OriGene) were transfected with reagents (TF81001;OriGene) for 12 h. The culture was then continued with a
Toxoplasma gondii infection and chronic IL-1 elevation drive hippocampal DNA double-strand break signaling, leading to cognitive deficits
Belloy, Schmitt, Marty et al
Nat Neurosci (2025)
Cited by 10
AI Summary: OriGene products, specifically the anti-Il1r1 shRNA and scramble control shRNA (TR30021) in the pGFP-C-shLenti shRNA vector (TR30023), were used to reduce IL-1R1 expression in neuronal cultures, enabling the study of IL-1 signaling's role in Toxoplasma gondii-induced cognitive deficits.
Snippet: Anti-Il1r1 and scramble (TR30021) shRNAs were obtained from Origene (pGFP-C-shLenti shRNA vector TR30023). Reduction in IL-1R1 mRNA levels was assessed in primary mouse neuronal cultures 1 week after transduction. The sequence of the effective shIL-1R1 is 5′-CCGTACTTTCTGATGGTGTTT-3′. Lentiviral particles were obtained as previously described53
Showing 1-6 of 32 papers.
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