pLenti-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector

Name: pLenti-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector
SKU: PS100092

CAT#: PS100092

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pLenti-C-Myc-DDK-P2A-Puro, Lenti vector with C-terminal Myc-DDK tag and P2A-Puro, 10 ug

Product Datasheet for 'PS100092'

Cited in 11 publications.

USD 750.00

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Specifications

Product Data
C-Tag	Myc-DDK
E. coli Selection	Chloramphenicol
Cell Selection	Puromycin
Features	This Lenti vector is a third generation lentiviral vector. All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF cloned in this vector can be expressed as a tagged protein with C-terminal Myc-DDK tags. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli selection: Chloramphenicol (34ug/ml) Download full vector sequence
Schematic of the multiple cloning sites

Citations (11)

The use of this Vectors has been cited in the following citations:
A kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury ,Kim, JY;Bai, Y;Jayne, LA;Hector, RD;Persaud, AK;Ong, SS;Rojesh, S;Raj, R;Feng, MJHH;Chung, S;Cianciolo, RE;Christman, JW;Campbell, MJ;Gardner, DS;Baker, SD;Sparreboom, A;Govindarajan, R;Singh, H;Chen, T;Poi, M;Susztak, K;Cobb, SR;Pabla, NS;, Nat Commun 2020 ,PubMed ID 32317630 [pLenti-C-Myc-DDK-P2A-Puro]
Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway ,Wang, Q;Zhang, N;Hu, L;Xi, Y;Mi, W;Ma, Y;, Tissue Eng Regen Med 2020 ,PubMed ID 32200515 [pLenti-C-Myc-DDK- P2A-Puro]
mTORC2/Rac1 Pathway Predisposes Cancer Aggressiveness in IDH1-Mutated Glioma ,Liu, Y;Lu, Y;Li, A;Celiku, O;Han, S;Qian, M;Yang, C;, Cancers (Basel) 2020 ,PubMed ID 32224866 [pLenti-C-Myc-DDK-P2A-Puro]
Oncogenic ERG represses PI3K signaling through down-regulation of IRS2 ,Mao, N;Gao, D;Hu, W;Gadal, S;Hieronymus, H;Wang, S;Lee, YS;Sullivan, P;Zhang, Z;Choi, D;Rosen, N;Sawyers, CL;Gopalan, A;Chen, Y;Carver, BS;, Cancer Res. 2020 ,PubMed ID 32015092 [pLenti-C-Myc-DDK-P2A-Puro]
PEDF-Rpsa-Itga6 signaling regulates cortical neuronal morphogenesis ,Blazejewski, SM;Bennison, SA;Ha, NT;Liu, X;Smith, TH;, bioRxiv 2020 [pLenti-C-Myc-DDK-P2A-Puro]
Peroxiredoxin 5 Silencing Sensitizes Dopaminergic Neuronal Cells to Rotenone via DNA Damage-Triggered ATM/p53/PUMA Signaling-Mediated Apoptosis ,Wang, MJ;Huang, HY;Chiu, TL;Chang, HF;Wu, HR;, Cells 2019 ,PubMed ID 31861721 [pLenti-C-Myc-DDK-P2A-Puro]
Mutations in the SPTLC1 gene are a cause of amyotrophic lateral sclerosis that may be amenable to serine supplementation ,Johnson, JO;Chia, R;Kumaran, R;Alahmady, N;, BioRxiv 2019 [pLenti-C-Myc-DDK-P2A-Puro]
Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer ,Su, H;Ren, F;Jiang, H;Chen, Y;, Oncology Letters 2019 ,PubMed ID 31452811 [pLenti-C-Myc-DDK-P2A-Puro]
Mutations in the Sphingolipid Pathway Gene SPTLC1 are a Cause of Amyotrophic Lateral Sclerosis ,Janel, J;Chia, R;Kumaran, R;Alahmady, N;Abramzon, Y;Faghri, F;Renton, A;Topp, S;Pliner, H;Gibbs, J;Ding, J;Smith, N;Landeck, N;Nalls, M;Cookson, M;Pletnikova, O;Troncoso, J;Scholz, S;Sabir, M;Ahmed, S;Dalgard, C;Troakes, C;Jones, A;Shatunov, A;Iacoangeli, A;Al Khleifat, A;Ticozzi, N;Silani, V;Gellera, C;Blair, I;Dobson-Stone, C;Kwok, J;England, B;Consortium, T;Consortium, T;Consortium, T;Center, T;Tienari, P;Stone, D;Morrison, K;Shaw, P;Al-Chalabi, A;Brown Jr., R;Brunetti, M;Calvo, A;Mora, G;Gotkine, M;Shaw, C;Landers, J;Chiò, A;Crawford, T;Smith, B;Traynor, B;, SSRN Journal 2019 [pLenti-C-Myc-DDK-P2A-Puro]
ONECUT2 is a driver of neuroendocrine prostate cancer ,Guo, H;Ci, X;Ahmed, M;Hua, JT;Soares, F;Lin, D;Puca, L;Vosoughi, A;Xue, H;Li, E;Su, P;Chen, S;Nguyen, T;Liang, Y;Zhang, Y;Xu, X;Xu, J;Sheahan, AV;Ba-Alawi, W;Zhang, S;Mahamud, O;Vellanki, RN;Gleave, M;Bristow, RG;Haibe-Kains, B;Poirier, JT;Rudin, CM;Tsao, MS;Wouters, BG;Fazli, L;Feng, FY;Ellis, L;van der Kwast, T;Berlin, A;Koritzinsky, M;Boutros, PC;Zoubeidi, A;Beltran, H;Wang, Y;He, HH;, Nat Commun 2019 ,PubMed ID 30655535 [pLenti-C-Myc-DDK-P2A-Puro]
Pleotropic effects of PPARD accelerate colorectal tumor progression and invasion ,Liu, Y;Deguchi, Y;Tian, R;Wei, D;Chen, W;Xu, M;Wu, L;Liu, F;Gao, S;Jaoude, J;Chrieki, S;Moussalli, M;Gagea, M;Morris, J;Broaddus, R;Zuo, X;Shureiqi, I;, bioRxiv 2018 [pLenti-C-Myc-DDK-P2A-Puro]

Documents

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS
ORF PrecisionShuttle Cloning Vector

Resources

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual

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