pLenti-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector

CAT#: PS100092

pLenti-C-Myc-DDK-P2A-Puro, Lenti vector with C-terminal Myc-DDK tag and P2A-Puro, 10 ug

Product images

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Specifications

Product Data

• C-Tag: Myc-DDK
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Features:

  This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with C-terminal Myc-DDK tags.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • P2A-DDK_R Primer can be used to sequence the target sequence after cloning.
• Schematic of the multiple cloning sites: