pLenti-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector

Name: pLenti-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector
SKU: PS100092

CAT#: PS100092

Write a review

pLenti-C-Myc-DDK-P2A-Puro, Lenti vector with C-terminal Myc-DDK tag and P2A-Puro, 10 ug

Datasheet

SDS

Citations (13)

USD 750.00

Availability*

In Stock

Size

Product images

Specifications

Product Data
C-Tag	Myc-DDK
E. coli Selection	Chloramphenicol
Cell Selection	Puromycin
Features	This Lenti vector is a third generation lentiviral vector. All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process. The ORF cloned in this vector can be expressed as a tagged protein with C-terminal Myc-DDK tags. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli selection: Chloramphenicol (34ug/ml) Download full vector sequence
Schematic of the multiple cloning sites

Citations (13)

The use of this Vectors has been cited in the following citations:
Correlation of Fibulin‑2 expression with proliferation, migration and invasion of breast cancer cells ,Zhang, X;Duan, L;Zhang, Y;Zhao, H;Yang, X;Zhang, C;, Oncol Lett 2020 ,PubMed ID 32724439 [pLenti-C-Myc-DDK-P2A-Puro]
Differential glucocorticoid-dependent regulation and function of the ERRFI1 gene in triple-negative breast cancer ,Mojica, CAR;Ybañez, WS;Olarte, KCV;Poblete, ABC;Bagamasbad, PD;, Endocrinology 2020 ,PubMed ID 32432675 [pLenti-C-Myc-DDK]
A kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury ,Kim, JY;Bai, Y;Jayne, LA;Hector, RD;Persaud, AK;Ong, SS;Rojesh, S;Raj, R;Feng, MJHH;Chung, S;Cianciolo, RE;Christman, JW;Campbell, MJ;Gardner, DS;Baker, SD;Sparreboom, A;Govindarajan, R;Singh, H;Chen, T;Poi, M;Susztak, K;Cobb, SR;Pabla, NS;, Nat Commun 2020 ,PubMed ID 32317630 [pLenti-C-Myc-DDK-P2A-Puro]
Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway ,Wang, Q;Zhang, N;Hu, L;Xi, Y;Mi, W;Ma, Y;, Tissue Eng Regen Med 2020 ,PubMed ID 32200515 [pLenti-C-Myc-DDK- P2A-Puro]
mTORC2/Rac1 Pathway Predisposes Cancer Aggressiveness in IDH1-Mutated Glioma ,Liu, Y;Lu, Y;Li, A;Celiku, O;Han, S;Qian, M;Yang, C;, Cancers (Basel) 2020 ,PubMed ID 32224866 [pLenti-C-Myc-DDK-P2A-Puro]
Oncogenic ERG represses PI3K signaling through down-regulation of IRS2 ,Mao, N;Gao, D;Hu, W;Gadal, S;Hieronymus, H;Wang, S;Lee, YS;Sullivan, P;Zhang, Z;Choi, D;Rosen, N;Sawyers, CL;Gopalan, A;Chen, Y;Carver, BS;, Cancer Res. 2020 ,PubMed ID 32015092 [pLenti-C-Myc-DDK-P2A-Puro]
PEDF-Rpsa-Itga6 signaling regulates cortical neuronal morphogenesis ,Blazejewski, SM;Bennison, SA;Ha, NT;Liu, X;Smith, TH;, bioRxiv 2020 [pLenti-C-Myc-DDK-P2A-Puro]
Peroxiredoxin 5 Silencing Sensitizes Dopaminergic Neuronal Cells to Rotenone via DNA Damage-Triggered ATM/p53/PUMA Signaling-Mediated Apoptosis ,Wang, MJ;Huang, HY;Chiu, TL;Chang, HF;Wu, HR;, Cells 2019 ,PubMed ID 31861721 [pLenti-C-Myc-DDK-P2A-Puro]
Mutations in the SPTLC1 gene are a cause of amyotrophic lateral sclerosis that may be amenable to serine supplementation ,Johnson, JO;Chia, R;Kumaran, R;Alahmady, N;, BioRxiv 2019 [pLenti-C-Myc-DDK-P2A-Puro]
Upregulation of microRNA‑520a‑3p inhibits the proliferation, migration and invasion via spindle and kinetochore associated 2 in gastric cancer ,Su, H;Ren, F;Jiang, H;Chen, Y;, Oncology Letters 2019 ,PubMed ID 31452811 [pLenti-C-Myc-DDK-P2A-Puro]
Mutations in the Sphingolipid Pathway Gene SPTLC1 are a Cause of Amyotrophic Lateral Sclerosis ,Janel, J;Chia, R;Kumaran, R;Alahmady, N;Abramzon, Y;Faghri, F;Renton, A;Topp, S;Pliner, H;Gibbs, J;Ding, J;Smith, N;Landeck, N;Nalls, M;Cookson, M;Pletnikova, O;Troncoso, J;Scholz, S;Sabir, M;Ahmed, S;Dalgard, C;Troakes, C;Jones, A;Shatunov, A;Iacoangeli, A;Al Khleifat, A;Ticozzi, N;Silani, V;Gellera, C;Blair, I;Dobson-Stone, C;Kwok, J;England, B;Consortium, T;Consortium, T;Consortium, T;Center, T;Tienari, P;Stone, D;Morrison, K;Shaw, P;Al-Chalabi, A;Brown Jr., R;Brunetti, M;Calvo, A;Mora, G;Gotkine, M;Shaw, C;Landers, J;Chiò, A;Crawford, T;Smith, B;Traynor, B;, SSRN Journal 2019 [pLenti-C-Myc-DDK-P2A-Puro]
ONECUT2 is a driver of neuroendocrine prostate cancer ,Guo, H;Ci, X;Ahmed, M;Hua, JT;Soares, F;Lin, D;Puca, L;Vosoughi, A;Xue, H;Li, E;Su, P;Chen, S;Nguyen, T;Liang, Y;Zhang, Y;Xu, X;Xu, J;Sheahan, AV;Ba-Alawi, W;Zhang, S;Mahamud, O;Vellanki, RN;Gleave, M;Bristow, RG;Haibe-Kains, B;Poirier, JT;Rudin, CM;Tsao, MS;Wouters, BG;Fazli, L;Feng, FY;Ellis, L;van der Kwast, T;Berlin, A;Koritzinsky, M;Boutros, PC;Zoubeidi, A;Beltran, H;Wang, Y;He, HH;, Nat Commun 2019 ,PubMed ID 30655535 [pLenti-C-Myc-DDK-P2A-Puro]
Pleotropic effects of PPARD accelerate colorectal tumor progression and invasion ,Liu, Y;Deguchi, Y;Tian, R;Wei, D;Chen, W;Xu, M;Wu, L;Liu, F;Gao, S;Jaoude, J;Chrieki, S;Moussalli, M;Gagea, M;Morris, J;Broaddus, R;Zuo, X;Shureiqi, I;, bioRxiv 2018 [pLenti-C-Myc-DDK-P2A-Puro]

Documents

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors

Resources

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual

Frequently bought together (2)

TA500002

c-Myc (c-Myc ) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)

USD 379.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

USD 430.00

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.