pLenti-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector

CAT#: PS100093

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pLenti-C-mGFP-P2A-Puro, Lenti vector with C-terminal mGFP tag and P2A-Puro, 10 ug

Datasheet
SDS
 Citations (12)

Save 30% with code “Vector”, ends on April 30th, 2021. View details.

USD 750.00

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Size
    • 10 ug

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Specifications

Product Data
C-Tag mGFP
E. coli Selection Chloramphenicol
Cell Selection Puromycin
Features

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with a C-terminal monomeric GFP tag.
  • P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli selection: Chloramphenicol (34ug/ml)
  • Download full vector sequence
Schematic of the multiple cloning sites

Citations (12)

The use of this Vectors has been cited in the following citations:
Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T Cells ,Hamaidi, I;Zhang, L;Kim, N;Wang, MH;Iclozan, C;Fang, B;Liu, M;Koomen, JM;Berglund, AE;Yoder, SJ;Yao, J;Engelman, RW;Creelan, BC;Conejo-Garcia, JR;Antonia, SJ;Mulé, JJ;Kim, S;, Cell Metab. 2020 ,PubMed ID 32768387 [pLenti-C-mGFP-P2A-Puro]
Exosomes Derived from Human Placental Mesenchymal Stromal Cells Carrying AntagomiR-4450 Alleviate Intervertebral Disc Degeneration Through Upregulation of ZNF121 ,Yuan, Q;Wang, X;Liu, L;Cai, Y;Zhao, X;Ma, H;Zhang, Y;, Stem Cells Dev. 2020 ,PubMed ID 32620067 [pLenti-C-mGFP-P2A-Puro]
Effects of pharmacological calcimimetics on colorectal cancer cells over-expressing the human calcium-sensing receptor ,Iamartino, L;Elajnaf, T;Gall, K;David, J;Manhardt, T;Heffeter, P;Grusch, M;Derdak, S;Baumgartner-Parzer, S;Schepelmann, M;Kallay, E;, bioRxiv 2020 [pLenti-C-mGFP-P2A-Puro]
Exosomes derived from human placental MSCs carrying miR-4450 inhibitor alleviate intervertebral disc degeneration and ameliorate gait disturbances via up-regulation of ZNF121 ,Yuan, Q;Wang, X;Liu, L;Cai, Y;Zhao, X;Ma, H;Zhang, Y;, Research Square 2020 [pLenti-C-mGFP-P2A-Puro]
Aberrant expression of CPSF1 promotes head and neck squamous cell carcinoma via regulating alternative splicing ,Sakai, A;Ando, M;Fukusumi, T;Ren, S;Liu, C;Qualliotine, J;Haft, S;Sadat, S;Saito, Y;Guo, TW;Xu, G;Sasik, R;Fisch, KM;Gutkind, JS;Fertig, EJ;Molinolo, AA;Califano, JA;, PLoS ONE 2020 ,PubMed ID 32437477 [pLenti-C-mGFP-P2A-Puro]
System χc- overexpression prevents 2-deoxy-d-ribose-induced β-cell damage ,Yoo, S;Bae, JY;Moon, J;Koh, G;, Free Radic. Biol. Med. 2020 ,PubMed ID 32305647 [pLenti-C-mGFP-P2A-Puro]
MYCN amplification and ATRX mutations are incompatible in neuroblastoma ,Zeineldin, M;Federico, S;Chen, X;Fan, Y;Xu, B;Stewart, E;Zhou, X;Jeon, J;Griffiths, L;Nguyen, R;Norrie, J;Easton, J;Mulder, H;Yergeau, D;Liu, Y;Wu, J;Van Ryn, C;Naranjo, A;Hogarty, MD;Kamiński, MM;Valentine, M;Pruett-Miller, SM;Pappo, A;Zhang, J;Clay, MR;Bahrami, A;Vogel, P;Lee, S;Shelat, A;Sarthy, JF;Meers, MP;George, RE;Mardis, ER;Wilson, RK;Henikoff, S;Downing, JR;Dyer, MA;, Nat Commun 2020 ,PubMed ID 32060267 [pLenti-C-mGFP-P2A-Puro]
Sensitivity of mesothelioma cells to PARP inhibitors is not dependent on BAP1 but is enhanced by temozolomide in cells with high-Schlafen 11and low-MGMT expression ,Rathkey, D;Khanal, M;Murai, J;Zhang, J;Sengupta, M;Jiang, Q;Morrow, B;Evans, CN;Chari, R;Fetsch, P;Chung, HJ;Xi, L;Roth, M;Filie, A;Raffeld, M;Thomas, A;Pommier, Y;Hassan, R;, J Thorac Oncol 2020 ,PubMed ID 32004714 [pLenti-C-mGFP- P2A-Puro]
Nrf2 and HIF1α converge to arsenic-induced metabolic reprogramming and the formation of the cancer stem-like cells ,Bi, Z;Zhang, Q;Fu, Y;Wadgaonkar, P;Zhang, W;, Theranostics 2020 ,PubMed ID 32226544 [pLenti-C-mGFP-P2A-Puro]
EMSY expression affects multiple components of skin barrier with relevance to atopic dermatitis ,Elias, M;Wright, S;Remenyi, J;Abbott, J;Bray, S;Cole, C;Edwards, S;Gierlinski, M;Glok, M;McGrath, J;Nicholson, W;Paternoster, L;Prescott, A;Have, S;Whitfield, P;Lamond, A;Brown, S;, Journal of Allergy and Clinical Immunology 2019 ,PubMed ID 31158401 [pLenti-C-mGFP-P2A-Puro]
Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity ,Lazzari, G;Nicolas, V;Matsusaki, M;Akashi, M;Couvreur, P;Mura, S;, Acta Biomater 2018 ,PubMed ID 30099198 [pLenti-C-mGFP-P2A-Puro]
Transient receptor potential vanilloid 5 (TRPV5), a highly Ca(2+) -selective TRP channel in the rat brain: relevance to neuroendocrine regulation ,Kumar, S;Singh, U;Goswami, C;Singru, PS;, J. Neuroendocrinol 2017 ,PubMed ID 28235149 [pLenti-C-mGFP-P2A-Puro]
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