Nek1 Mouse siRNA Oligo Duplex (Locus ID 18004)

SKU SR422391

Nek1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Product Data

• Purity: HPLC purified
• Quality Control: Tested by ESI-MS
• Sequences: Available with shipment
• Components:
  Nek1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 18004); Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol; Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml

  Once you identified the best-performing siRNA sequence of the kit, you can order a single siRNA individually.

  Cat#	SRXXXXXX followed by A, B or C, then add L; For example, for sequence A of SR300003, the SKU is SR300003AL.
  Description	10 nmol of siRNA of the above specified vial, scramble control NOT included
  Price	$/€ 375 per vial

  If you want to order 20 nmol, please order quantity of 2 units.; If you need negative control, please order SR30004 (2 nmol of scramble siRNA control)
  Need an individual siRNA?
• Stability: One year from date of shipment when stored at -20°C.
• Shipping: Ambient
• # of Transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
• Note: Single siRNA duplex (10nmol) can be ordered.

Reference Data

• RefSeq: NM_001293637, NM_001293638, NM_001293639, NM_175089
• UniProt ID: P51954
• Synonyms: D8Ertd790e; kat
• Summary: Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed:1382974). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed:18843199). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (By similarity). May be implicated in the control of meiosis (PubMed:1382974). Involved in cilium assembly (By similarity).[UniProtKB/Swiss-Prot Function]
• Performance Guaranteed: OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.; ; For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).