NRARP Human siRNA Oligo Duplex (Locus ID 441478)

SKU SR318569

NRARP (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Product Data

• Purity: HPLC purified
• Quality Control: Tested by ESI-MS
• Sequences: Available with shipment
• Components:
  NRARP (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 441478); Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol; Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml

  Once you identified the best-performing siRNA sequence of the kit, you can order a single siRNA individually.

  Cat#	SRXXXXXX followed by A, B or C, then add L; For example, for sequence A of SR300003, the SKU is SR300003AL.
  Description	10 nmol of siRNA of the above specified vial, scramble control NOT included
  Price	$/€ 375 per vial

  If you want to order 20 nmol, please order quantity of 2 units.; If you need negative control, please order SR30004 (2 nmol of scramble siRNA control)
  Need an individual siRNA?
• Stability: One year from date of shipment when stored at -20°C.
• Shipping: Ambient
• # of Transfections: Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
• Note: Single siRNA duplex (10nmol) can be ordered.

Reference Data

• RefSeq: NM_001004354
• UniProt ID: Q7Z6K4
• Summary: Downstream effector of Notch signaling. Involved in the regulation of liver cancer cells self-renewal (PubMed:25985737). Involved in angiogenesis acting downstream of Notch at branch points to regulate vascular density. Proposed to integrate endothelial Notch and Wnt signaling to control stalk cell proliferation and to stablilize new endothelial connections during angiogenesis (PubMed:19154719). During somitogenesis involved in maintenance of proper somite segmentation and proper numbers of somites and vertebrae. Required for proper anterior-posterior somite patterning. Proposed to function in a negative feedback loop to destabilize Notch 1 intracellular domain (NICD) and downregulate the Notch signal, preventing expansion of the Notch signal into the anterior somite domain (By similarity).[UniProtKB/Swiss-Prot Function]
• Performance Guaranteed: OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.; ; For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).