SGPL1 (NM_003901) Human Recombinant Protein
$737.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 63.3 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_003892 |
| Locus ID | 8879 |
| UniProt ID | O95470 |
| Cytogenetics | 10q22.1 |
| RefSeq Size | 4701 |
| RefSeq ORF | 1704 |
| Synonyms | NPHS14; S1PL; SPL |
| Summary | Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).UniProtKB/Swiss-Prot Function |
| Protein Categories | Growth Factors, Intracellular Proteins, Membrane Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "SGPL1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH308705 | SGPL1 MS Standard C13 and N15-labeled recombinant protein (NP_003892) | 10 ug |
$3,360.00
|
|
| LC418357 | Lyophilized SGPL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY418357 | Transient overexpression lysate of sphingosine-1-phosphate lyase 1 (SGPL1) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Site-Dependent Cysteine Lipidation Potentiates the Activation of Proapoptotic BAX
Cohen, Wales, McHenry et al
Cell Rep (2020) 30 (10), 3229-3239.e6 DOI: 10.1016/j.celrep.2020.02.057
Cohen, Wales, McHenry et al
Cell Rep (2020) 30 (10), 3229-3239.e6 DOI: 10.1016/j.celrep.2020.02.057
Cited by 33
AI Summary: OriGene’s SGPL1 Human Recombinant Protein (Cat# TP308705) was used in the study to investigate the enzymatic regulation of BAX activation and lipidation during apoptosis.
Snippet: PES, 1 mM EDTA, pH 7.4, complete protease inhibitor cocktail [Roche]) with mitochondria (1 μg/mL), BIM SAHBA2 (500 nM) or vehicle (0.05% DMSO), in the presence or absence of recombinant SGPL1 (1 mg, Origene), for 2 h at 37°C. Reactions were diluted in an equal volume of BAX FPLC buffer containing 2% CHAPS, followed by addition of propargyl hydrazine (1.25 μL of 10 mM solution) and 30 min incuba;Recombinant BAX WT Walensky Lab N/A Recombinant BAX C62A Walensky Lab N/A Recombinant BAX C126A Walensky Lab N/A SGPL1 Human Recombinant Protein Origene Cat# TP308705
First evidence of SGPL1 expression in the cell membrane silencing the extracellular S1P siren in mammary epithelial cells
Engel, Adamus, Frank et al
PLoS ONE (2018) 13 (5), e0196854 DOI: 10.1371/journal.pone.0196854
Engel, Adamus, Frank et al
PLoS ONE (2018) 13 (5), e0196854 DOI: 10.1371/journal.pone.0196854
AI Summary: OriGene’s TrueORF clone RC208705, encoding full-length human SGPL1 with a C-terminal DDK tag, was used to produce SGPL1 protein for expression and functional studies in this research.
Snippet: lyase 1 (SGPL1) was produced with TrueORF clone, RC208705 encoding the full-length human SGPL1 with C-terminal DDK tag, from human HEK293 cells (GenBank accession: NM_003901, Predicted molecular weight: 63.3 kDa, purchased from OriGene Technologies Cached
Showing 1-2 of 2 papers.
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in shipping.
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