RNF2 (NM_007212) Human Recombinant Protein
$7,820.00
6 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 37.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_009143 |
| Locus ID | 6045 |
| UniProt ID | Q99496 |
| Cytogenetics | 1q25.3 |
| RefSeq Size | 3551 |
| RefSeq ORF | 1008 |
| Synonyms | BAP-1; BAP1; DING; HIPI3; RING1B; RING2 |
| Summary | Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. provided by RefSeq, Jul 2008 |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome, Transcription Factors |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
TRIM37 is a new histone H2A ubiquitin ligase and breast cancer oncoprotein
Bhatnagar, Gazin, Chamberlain et al
Nature (2014)
Bhatnagar, Gazin, Chamberlain et al
Nature (2014)
AI Summary: OriGene’s RNF2 protein was used in in vitro ubiquitination assays to investigate the ubiquitin ligase activity of TRIM37 in the study.
Snippet: In vitro ubiquitination reactions were set up containing purified TRIM37 or TRIM37(C18R) (500 ng to 2 µg), a combination of BMI1 (500 ng;Abcam) and RNF2 (500 ng;Origene) mixed together, or BRCA1 (500 ng;Abcam) plus H2A or H2B (5X;Boston Biochemicals), 10 nM UBE1 (E1;Boston Biochemicals), 10 nM UBCH5B (E2;Boston Biochemicals), and 10 nM HA-ubiquitin aldehyde (Boston Biochemicals) in 10 µl of reaction buffer (50 mM Tris-Cl, pH 7.9, 5 mM MgCl2, 2 mM NaF, 100 mM dithiothreitol (DTT) and 2 mM ATP)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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