GLI1 (NM_005269) Human Over-expression Lysate

CAT#: LC417412

GLI1 HEK293T cell transient overexpression lysate (as WB positive control)


 Product Datasheet for 'LC417412'

  View other "GLI1" proteins (3)

Use code "PROTEIN10" to save 10%

USD 95.00


Availability*
In Stock

Size
    • 20 ug


Product images

Specifications

Product Data
Description GLI1 HEK293T cell transient overexpression lysate (as WB positive control)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201110
Tag C-MYC/DDK
Detection Antibodies Anti-DDK Antibody (TA50011-100)
Accession Number NM_005269, NP_005260
Other Names GLI1, GLI; GLI
Predicted MW 117.9 kDa
Components 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
RefSeq NP_005260
Locus ID 2735
Cytogenetics GLI; GLI
Protein Families Stem cell relevant signaling - DSL/Notch pathway, ES Cell Differentiation/IPS, Adult stem cells, Cancer stem cells, Embryonic stem cells, Transcription Factors, Druggable Genome
Protein Pathways Hedgehog signaling pathway, Pathways in cancer, Basal cell carcinoma

Other Versions

Other products for "GLI1"
Frequently bought together (3)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

USD 299.00


GLI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2)
    • 100 ul

USD 360.00


beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

USD 150.00

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
10 percent off protein banner ad
68 Mouse Clones
20%off selected tag antibodies