Trpv4 Mouse Gene Knockout Kit (CRISPR)

SKU
KN318311RB
Trpv4 - mouse gene knockout kit via CRISPR, HDR mediated
$1,657.00
4 Weeks*
Specifications
Product Data
Format 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Vector RFP-BSD
Target Symbol Trpv4
Locus ID 63873
Components

KN318311G1, Trpv4 gRNA vector 1 in pCas-Guide CRISPR vector

KN318311G2, Trpv4 gRNA vector 2 in pCas-Guide CRISPR vector

KN318311RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

GE100003, scramble sequence in pCas-Guide vector

OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_022017
UniProt ID Q9EPK8
Synonyms 0610033B08Rik; OTRPC4; Trp12; VR-OAC; VRL-2; VROAC
Summary Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:11094154). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:12093812, PubMed:14691263, PubMed:16368742, PubMed:16571723). Also activated by heat, low pH, citrate and phorbol esters (PubMed:14691263). Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (PubMed:20413591). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (PubMed:23021218). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (PubMed:23021218). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723). Required for normal development and maintenance of bone and cartilage (By similarity).[UniProtKB/Swiss-Prot Function]
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More linear donor DNA can be ordered separately

Cat# KN318311RBD
Description 10 ug linear donor DNA for KN318311RB kit
Price $515/€515
If you want to order 20ug, please order quantity of 2 for KN318311RBD
If you want to order 50ug, please order quantity of 5 for KN318311RBD

Other selection marker is available for donor DNA.
Please contact techsupport@origene.com for a custom quote.

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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.