Mouse Xist activation kit by CRISPRa

SKU GA213173

Xist CRISPRa kit - CRISPR gene activation of mouse inactive X specific transcripts

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Xist
• Locus ID: 213742
• Components:

  GA213173G1, Xist gRNA vector 1 in pCas-Guide-GFP-CRISPRa, Target Sequence: AACTTCTAGTGTCCAAGACG

  GA213173G2, Xist gRNA vector 2 in pCas-Guide-GFP-CRISPRa, Target Sequence: TATAATATAGTTAGACCTAA

  GA213173G3, Xist gRNA vector 3 in pCas-Guide-GFP-CRISPRa, Target Sequence: TTGCAATTCTGACATCTTAT

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NR_001463, NR_001570
• Summary: X inactivation is an early developmental process in mammalian females that transcriptionally silences one of the pair of X chromosomes, thus providing dosage equivalence between males and females. The process is regulated by several factors, including a region of chromosome X called the X inactivation center (XIC). The XIC comprises several non-coding and protein-coding genes, and this gene was the first non-coding gene identified within the XIC. This gene is expressed exclusively from the XIC of the inactive X chromosome. It is essential for the initiation and spread of X-inactivation. It silences hundreds of genes by recruiting and tethering repressive chromatin-modifying complexes to the inactive X chromosome. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]