Mouse Huwe1 activation kit by CRISPRa

SKU GA206339

Huwe1 CRISPRa kit - CRISPR gene activation of mouse HECT, UBA and WWE domain containing 1

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Target Symbol: Huwe1
• Locus ID: 59026
• Components:

  GA206339G1, Huwe1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA206339G2, Huwe1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA206339G3, Huwe1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021523
• Synonyms: 5430439H10Rik; Arf-bp1; AU041296; C80292; C430014N20Rik; Gm1718; Ib772; LASU1; Mule; Ureb1
• Summary: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene (By similarity). Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of PA2G4 (By similarity). Acts in concert with MYCBP2 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1 (By similarity).[UniProtKB/Swiss-Prot Function]