Glucosidase 2 subunit beta (PRKCSH) (NM_001289103) Human Untagged Clone

SKU SC336666

PRKCSH (untagged) - Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: Glucosidase 2 subunit beta
• Synonyms: AGE-R2; G19P1; GIIB; PCLD; PCLD1; PKCSH; PLD1; VASAP-60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336666 representing NM_001289103.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTGTTGCCGCTGCTGCTGCTGCTACCCATGTGCTGGGCCGTGGAGGTCAAGAGGCCCCGGGGCGTC; TCCCTCACCAATCATCACTTCTACGATGAGTCCAAGCCTTTCACCTGCCTGGACGGTTCGGCCACCATC; CCATTTGATCAGGTCAACGATGACTATTGCGACTGCAAAGATGGCTCTGACGAGCCAGGCACGGCTGCC; TGTCCTAATGGCAGCTTCCACTGCACCAACACTGGCTATAAGCCCCTGTATATCCCCTCCAACCGGGTC; AACGATGGTGTTTGTGACTGCTGCGATGGAACAGACGAGTACAACAGCGGCGTCATCTGTGAGAACACC; TGCAAAGAGAAGGGCCGTAAGGAGAGAGAGTCCCTGCAGCAGATGGCCGAGGTCACCCGCGAAGGGTTC; CGTCTGAAGAAGATCCTTATTGAGGACTGGAAGAAGGCACGGGAGGAGAAGCAGAAAAAGCTCATTGAG; CTACAGGCTGGGAAGAAGTCTCTGGAAGACCAGGTGGAGATGCTGCGGACAGTGAAGGAGGAAGCTGAG; AAGCCAGAGAGAGAGGCCAAAGAGCAGCACCAGAAGCTGTGGGAAGAGCAGCTGGCTGCTGCCAAGGCC; CAACAGGAGCAGGAGCTGGCGGCTGATGCCTTCAAGGAGCTGGATGATGACATGGACGGGACGGTCTCG; GTGACTGAGCTGCAGACTCACCCGGAGCTGGACACAGATGGGGATGGGGCGTTGTCAGAAGCGGAAGCT; CAGGCCCTCCTCAGTGGGGACACACAGACAGACGCCACCTCTTTCTACGACCGCGTCTGGGCCGCCATC; AGGGACAAGTACCGGTCCGAGGCACTGCCCACCGACCTTCCAGCACCTTCTGCCCCTGACTTGACGGAG; CCCAAGGAGGAGCAGCCGCCAGTGCCCTCGTCGCCCACAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAG; GAAGAAGAGGCTGAAGAAGAGGAGGAGGAGGAGGATTCCGAGGTGCAGGGGGAGCAGCCCAAGGAGGCC; CCACCGCCACTGTCACCCCCGCAGCCGGCCAGCCCTGCTGAGGAAGACAAAATGCCGCCCTACGACGAG; CAGACGCAGGCCTTCATCGATGCTGCCCAGGAGGCCCGCAACAAGTTCGAGGAGGCCGAGCGGTCGCTG; AAGGACATGGAGGAGTCCATCAGGAACCTGGAGCAAGAGATTTCTTTTGACTTTGGCCCCAACGGGGAG; TTTGCTTACCTGTACAGCCAGTGCTACGAGCTCACCACCAACGAATACGTCTACCGCCTCTGCCCCTTC; AAGCTTGTCTCGCAGAAACCCAAACTCGGGGGCTCTCCCACCAGCCTTGGCACCTGGGGCTCATGGATT; GGCCCCGACCACGACAAGTTCAGTGCCATGAAGTATGAGCAAGGCACGGGCTGCTGGCAGGGCCCCAAC; CGCTCCACCACCGTGCGCCTCCTGTGCGGGAAAGAGACCATGGTGACCAGCACCACAGAGCCCAGTCGC; TGCGAGTACCTCATGGAGCTGATGACGCCAGCCGCCTGCCCGGAGCCACCGCCTGAAGCACCCACCGAA; GACGACCATGACGAGCTCTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289103
• Insert Size: 1608 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001289103.1
• RefSeq Size: 2492 bp
• RefSeq ORF: 1608 bp
• Locus ID: 5589
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome
• MW: 60.2 kDa
• Summary: This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]; Transcript Variant: This variant (4) differs in its 5' UTR, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is longer, compared to isoform 1. Both variants 4 and 5 encode the same isoform.