Glucosidase 2 subunit beta (PRKCSH) (NM_001289103) Human Tagged ORF Clone

SKU RG238772

PRKCSH (tGFP-tagged) - Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 4

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Glucosidase 2 subunit beta
• Synonyms: AGE-R2; G19P1; GIIB; PCLD; PCLD1; PKCSH; PLD1; VASAP-60
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238772 representing NM_001289103.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTGTTGCCGCTGCTGCTGCTGCTACCCATGTGCTGGGCCGTGGAGGTCAAGAGGCCCCGGGGCGTC; TCCCTCACCAATCATCACTTCTACGATGAGTCCAAGCCTTTCACCTGCCTGGACGGTTCGGCCACCATC; CCATTTGATCAGGTCAACGATGACTATTGCGACTGCAAAGATGGCTCTGACGAGCCAGGCACGGCTGCC; TGTCCTAATGGCAGCTTCCACTGCACCAACACTGGCTATAAGCCCCTGTATATCCCCTCCAACCGGGTC; AACGATGGTGTTTGTGACTGCTGCGATGGAACAGACGAGTACAACAGCGGCGTCATCTGTGAGAACACC; TGCAAAGAGAAGGGCCGTAAGGAGAGAGAGTCCCTGCAGCAGATGGCCGAGGTCACCCGCGAAGGGTTC; CGTCTGAAGAAGATCCTTATTGAGGACTGGAAGAAGGCACGGGAGGAGAAGCAGAAAAAGCTCATTGAG; CTACAGGCTGGGAAGAAGTCTCTGGAAGACCAGGTGGAGATGCTGCGGACAGTGAAGGAGGAAGCTGAG; AAGCCAGAGAGAGAGGCCAAAGAGCAGCACCAGAAGCTGTGGGAAGAGCAGCTGGCTGCTGCCAAGGCC; CAACAGGAGCAGGAGCTGGCGGCTGATGCCTTCAAGGAGCTGGATGATGACATGGACGGGACGGTCTCG; GTGACTGAGCTGCAGACTCACCCGGAGCTGGACACAGATGGGGATGGGGCGTTGTCAGAAGCGGAAGCT; CAGGCCCTCCTCAGTGGGGACACACAGACAGACGCCACCTCTTTCTACGACCGCGTCTGGGCCGCCATC; AGGGACAAGTACCGGTCCGAGGCACTGCCCACCGACCTTCCAGCACCTTCTGCCCCTGACTTGACGGAG; CCCAAGGAGGAGCAGCCGCCAGTGCCCTCGTCGCCCACAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAG; GAAGAAGAGGCTGAAGAAGAGGAGGAGGAGGAGGATTCCGAGGTGCAGGGGGAGCAGCCCAAGGAGGCC; CCACCGCCACTGTCACCCCCGCAGCCGGCCAGCCCTGCTGAGGAAGACAAAATGCCGCCCTACGACGAG; CAGACGCAGGCCTTCATCGATGCTGCCCAGGAGGCCCGCAACAAGTTCGAGGAGGCCGAGCGGTCGCTG; AAGGACATGGAGGAGTCCATCAGGAACCTGGAGCAAGAGATTTCTTTTGACTTTGGCCCCAACGGGGAG; TTTGCTTACCTGTACAGCCAGTGCTACGAGCTCACCACCAACGAATACGTCTACCGCCTCTGCCCCTTC; AAGCTTGTCTCGCAGAAACCCAAACTCGGGGGCTCTCCCACCAGCCTTGGCACCTGGGGCTCATGGATT; GGCCCCGACCACGACAAGTTCAGTGCCATGAAGTATGAGCAAGGCACGGGCTGCTGGCAGGGCCCCAAC; CGCTCCACCACCGTGCGCCTCCTGTGCGGGAAAGAGACCATGGTGACCAGCACCACAGAGCCCAGTCGC; TGCGAGTACCTCATGGAGCTGATGACGCCAGCCGCCTGCCCGGAGCCACCGCCTGAAGCACCCACCGAA; GACGACCATGACGAGCTC; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC;
  Protein Sequence

  >Peptide sequence encoded by RG238772; Blue=ORF Red=Cloning site Green=Tag(s); ; MLLPLLLLLPMCWAVEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAA; CPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGF; RLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKA; QQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAI; RDKYRSEALPTDLPAPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEEAEEEEEEEDSEVQGEQPKEA; PPPLSPPQPASPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGE; FAYLYSQCYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPN; RSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL; TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV; MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED; SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA; FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV;
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289103
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001289103.1, NP_001276032.1
• RefSeq Size: 2492 bp
• RefSeq ORF: 1608 bp
• Locus ID: 5589
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome
• MW: 60.6 kDa
• Summary: This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]