Dystrobrevin beta (DTNB) (NM_001256308) Human Untagged Clone

SKU SC332342

DTNB (untagged) - Homo sapiens dystrobrevin, beta (DTNB), transcript variant 8

Product Data

• Type: Human Untagged Clone
• Target Symbol: Dystrobrevin beta
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332342 representing NM_001256308.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGATTGAAGCCTTCCGAGACAATGGCCTTAATACACTGGACCATACCACCGAGATCAGTGTGTCCCGC; CTCGAAACTGTCATCTCCTCCATCTACTATCAGTTGAACAAGCGCCTTCCTTCTACTCACCAAATTAGT; GTGGAACAATCTATCAGCCTCCTCCTCAACTTTATGATTGCTGCATATGACAGTGAGGGCCGAGGCAAG; TTGACGGTATTTTCAGTTAAAGCTATGTTAGCAACCATGTGTGGTGGAAAAATGCTGGACAAATTGAGA; TATGTTTTCTCCCAGATGTCAGATTCCAATGGCTTAATGATATTTAGCAAGTTTGACCAGTTTCTGAAG; GAAGTTCTGAAGCTCCCAACAGCTGTCTTTGAAGGGCCATCTTTTGGTTACACAGAGCACTCAGTCCGC; ACCTGTTTTCCACAGCAGAGAAAGATAATGCTAAATATGTTTTTAGACACAATGATGGCTGACCCTCCT; CCCCAGTGCCTTGTCTGGCTACCTCTCATGCACAGGCTTGCCCATGTTGAGAATGTCTTCCATCCCGTG; GAGTGCTCCTACTGCCGATGTGAGAGTATGATGGGTTTCCGGTACCGATGCCAGCAGTGCCACAACTAT; CAGCTCTGCCAGAATTGCTTTTGGCGTGGCCATGCCGGCGGCCCTCACAGCAACCAGCACCAGATGAAG; GAGCATTCCTCTTGGAAATCTCCTGCAAAGAAGCTGAGCCATGCAATTAGTAAATCTTTGGGGTGTGTA; CCCACGAGAGAACCCCCGCATCCTGTTTTTCCTGAGCAACCAGAGAAACCACTTGACCTTGCACATATA; GTTCCTCCTCGCCCTCTGACTAATATGAATGACACCATGGTTAGCCACATGTCCTCTGGAGTGCCCACT; CCCACCAAGAGGTTACAGTATAGCCAGGATATACCCAGTCACTTGGCCGATGAGCATGCGCTGATAGCC; TCCTATGTGGCCCGTCTGCAGCACTGTGCACGTGTTCTGGACAGTCCTAGCCGACTGGATGAGGAACAC; CGTCTTATAGCTCGCTATGCTGCCCGGCTGGCTGCAGAAGCAGGAAACGTGACTCGTCCTCCCACTGAC; TTGAGCTTTAACTTTGATGCCAACAAACAACAAAGACAGCTTATTGCAGAACTGGAAAACAAAAACAGA; GAGATCCTGCAGGAGATTCAGCGTCTCCGCCTGGAACACGAGCAGGCCTCCCAGCCCACCCCTGAGAAG; GCACAGCAGAACCCCACGCTGCTGGCAGAGCTGCGGCTGCTGAGGCAAAGGAAGGATGAACTGGAGCAG; AGGATGTCGGCCCTGCAGGAGAGCAGGCGGGAGCTGATGGTCCAGCTGGAAGAGCTGATGAAGTTGCTG; AAGGCTCAGGCCACAGGGTCACCACATACATCGCCCACCCATGGAGGCGGCCGGCCAATGCCCATGCCA; GTGCGCTCCACGTCTGCCGGCTCCACCCCCACCCACTGTCCGCAGGACTCGCTGAGCGGAGTCGGGGGA; GACGTGCAGGAGGCCTTCGCACAAGGTACGAGGAGAAACCTCCGCAATGACCTGCTGGTGGCAGCTGAC; TCCATCACCAACACCATGTCATCCCTGGTGAAGGAGCTCCATTCAGGTTAG;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256308
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001256308.1
• RefSeq Size: 2328 bp
• RefSeq ORF: 1638 bp
• Locus ID: 1838
• UniProt ID: O60941
• Cytogenetics: 2p23.3
• MW: 61.5 kDa
• Summary: This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (8) differs in the 5' UTR, lacks a portion of the 5' coding region, initiates translation at a downstream AUG start codon, and lacks two in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform