Dystrobrevin beta (DTNB) (NM_001256308) Human Tagged ORF Clone

SKU RG234400

DTNB (tGFP-tagged) - Homo sapiens dystrobrevin, beta (DTNB), transcript variant 8

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Dystrobrevin beta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234400 representing NM_001256308; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATTGAAGCCTTCCGAGACAATGGCCTTAATACACTGGACCATACCACCGAGATCAGTGTGTCCCGCC; TCGAAACTGTCATCTCCTCCATCTACTATCAGTTGAACAAGCGCCTTCCTTCTACTCACCAAATTAGTGT; GGAACAATCTATCAGCCTCCTCCTCAACTTTATGATTGCTGCATATGACAGTGAGGGCCGAGGCAAGTTG; ACGGTATTTTCAGTTAAAGCTATGTTAGCAACCATGTGTGGTGGAAAAATGCTGGACAAATTGAGATATG; TTTTCTCCCAGATGTCAGATTCCAATGGCTTAATGATATTTAGCAAGTTTGACCAGTTTCTGAAGGAAGT; TCTGAAGCTCCCAACAGCTGTCTTTGAAGGGCCATCTTTTGGTTACACAGAGCACTCAGTCCGCACCTGT; TTTCCACAGCAGAGAAAGATAATGCTAAATATGTTTTTAGACACAATGATGGCTGACCCTCCTCCCCAGT; GCCTTGTCTGGCTACCTCTCATGCACAGGCTTGCCCATGTTGAGAATGTCTTCCATCCCGTGGAGTGCTC; CTACTGCCGATGTGAGAGTATGATGGGTTTCCGGTACCGATGCCAGCAGTGCCACAACTATCAGCTCTGC; CAGAATTGCTTTTGGCGTGGCCATGCCGGCGGCCCTCACAGCAACCAGCACCAGATGAAGGAGCATTCCT; CTTGGAAATCTCCTGCAAAGAAGCTGAGCCATGCAATTAGTAAATCTTTGGGGTGTGTACCCACGAGAGA; ACCCCCGCATCCTGTTTTTCCTGAGCAACCAGAGAAACCACTTGACCTTGCACATATAGTTCCTCCTCGC; CCTCTGACTAATATGAATGACACCATGGTTAGCCACATGTCCTCTGGAGTGCCCACTCCCACCAAGAGGT; TACAGTATAGCCAGGATATACCCAGTCACTTGGCCGATGAGCATGCGCTGATAGCCTCCTATGTGGCCCG; TCTGCAGCACTGTGCACGTGTTCTGGACAGTCCTAGCCGACTGGATGAGGAACACCGTCTTATAGCTCGC; TATGCTGCCCGGCTGGCTGCAGAAGCAGGAAACGTGACTCGTCCTCCCACTGACTTGAGCTTTAACTTTG; ATGCCAACAAACAACAAAGACAGCTTATTGCAGAACTGGAAAACAAAAACAGAGAGATCCTGCAGGAGAT; TCAGCGTCTCCGCCTGGAACACGAGCAGGCCTCCCAGCCCACCCCTGAGAAGGCACAGCAGAACCCCACG; CTGCTGGCAGAGCTGCGGCTGCTGAGGCAAAGGAAGGATGAACTGGAGCAGAGGATGTCGGCCCTGCAGG; AGAGCAGGCGGGAGCTGATGGTCCAGCTGGAAGAGCTGATGAAGTTGCTGAAGGCTCAGGCCACAGGGTC; ACCACATACATCGCCCACCCATGGAGGCGGCCGGCCAATGCCCATGCCAGTGCGCTCCACGTCTGCCGGC; TCCACCCCCACCCACTGTCCGCAGGACTCGCTGAGCGGAGTCGGGGGAGACGTGCAGGAGGCCTTCGCAC; AAGGTACGAGGAGAAACCTCCGCAATGACCTGCTGGTGGCAGCTGACTCCATCACCAACACCATGTCATC; CCTGGTGAAGGAGCTCCATTCAGGT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234400 representing NM_001256308; Red=Cloning site Green=Tags(s); ; MIEAFRDNGLNTLDHTTEISVSRLETVISSIYYQLNKRLPSTHQISVEQSISLLLNFMIAAYDSEGRGKL; TVFSVKAMLATMCGGKMLDKLRYVFSQMSDSNGLMIFSKFDQFLKEVLKLPTAVFEGPSFGYTEHSVRTC; FPQQRKIMLNMFLDTMMADPPPQCLVWLPLMHRLAHVENVFHPVECSYCRCESMMGFRYRCQQCHNYQLC; QNCFWRGHAGGPHSNQHQMKEHSSWKSPAKKLSHAISKSLGCVPTREPPHPVFPEQPEKPLDLAHIVPPR; PLTNMNDTMVSHMSSGVPTPTKRLQYSQDIPSHLADEHALIASYVARLQHCARVLDSPSRLDEEHRLIAR; YAARLAAEAGNVTRPPTDLSFNFDANKQQRQLIAELENKNREILQEIQRLRLEHEQASQPTPEKAQQNPT; LLAELRLLRQRKDELEQRMSALQESRRELMVQLEELMKLLKAQATGSPHTSPTHGGGRPMPMPVRSTSAG; STPTHCPQDSLSGVGGDVQEAFAQGTRRNLRNDLLVAADSITNTMSSLVKELHSG; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256308
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001256308.2
• RefSeq Size: 2328 bp
• RefSeq ORF: 1638 bp
• Locus ID: 1838
• UniProt ID: O60941
• Cytogenetics: 2p23.3
• Summary: This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. [provided by RefSeq, Mar 2016]