DENND1B (NM_144977) Human Untagged Clone

SKU SC327849

DENND1B (untagged)-Human DENN/MADD domain containing 1B (DENND1B) transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DENND1B
• Synonyms: C1ORF18; C1orf218; FAM31B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327849 representing NM_144977.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGACTGCAGGACCAAGGCAAATCCAGACAGAACCTTTGACTTGGTGTTGAAAGTGAAATGTCATGCC; TCTGAAAATGAAGATCCTGTGGTATTGTGGAAATTCCCAGAGGACTTTGGAGACCAGGAAATACTACAG; AGTGTGCCAAAGTTCTGTTTTCCCTTTGACGTTGAAAGGGTGTCTCAGAATCAAGTTGGACAGCACTTT; ACCTTTGTACTGACAGACATTGAAAGTAAACAGAGATTTGGATTCTGCAGACTGACGTCAGGAGGCACA; ATTTGTTTATGCATCCTTAGTTACCTTCCCTGGTTTGAAGTGTATTACAAGCTTCTAAATACTCTTGCA; GATTACTTGGCTAAGGAACTGGAAAATGATTTGAATGAAACTCTCAGATCACTGTATAACCACCCAGTA; CCAAAGGCAAATACTCCTGTAAATTTGAGTGTGAACCAAGAGATATTTATTGCCTGTGAGCAAGTTCTG; AAAGATCAGCCTGCTCTAGTACCGCATTCCTACTTCATTGCCCCTGATGTAACTGGACTCCCAACAATA; CCCGAGAGTAGAAATCTTACAGAATATTTTGTTGCCGTGGATGTGAACAACATGCTGCAGCTGTATGCC; AGTATGCTGCATGAAAGGCGCATCGTGATTATCTCGAGCAAATTAAGCACTTTAACTGCCTGTATCCAT; GGATCAGCTGCTCTTCTATACCCAATGTATTGGCAACACATATACATCCCAGTGCTTCCTCCACACCTG; CTGGACTACTGCTGTGCCCCAATGCCATACCTGATTGGAATACACTCCAGCCTCATAGAGAGAGTGAAA; AACAAATCATTGGAAGATGTTGTTATGTTAAATGTTGATACAAACACATTAGAATCACCATTTAGTGAC; TTGAACAACCTACCAAGTGATGTGGTCTCGGCCTTGAAAAATAAACTGAAGAAGCAGTCTACAGCTACG; GGTGATGGAGTAGCTAGGGCCTTTCTTAGAGCACAGGCTGCTTTGTTTGGATCCTACAGAGATGCACTG; AGATACAAACCTGGTGAGCCCATCACTTTCTGTGAGGAGAGTTTTGTAAAGCACCGCTCAAGCGTGATG; AAACAGTTCCTGGAAACTGCCATTAACCTCCAGCTTTTTAAGCAGTTTATCGATGGTCGACTGGCAAAA; CTAAATGCAGGAAGGGGTTTCTCTGATGTATTTGAAGAAGAGATCACTTCAGGTGGCTTTTGTGGAGGT; AAAGACAAGTTACAATATGATTATCCATTTTCTCAATAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_144977
• Insert Size: 1281 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144977.4
• RefSeq Size: 2380 bp
• RefSeq ORF: 1281 bp
• Locus ID: 163486
• UniProt ID: Q6P3S1
• Cytogenetics: 1q31.3
• Protein Families: Transmembrane
• MW: 48.3 kDa
• Summary: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]; Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 3. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 3.