DENND1B (NM_144977) Human Tagged ORF Clone

SKU RG205585

DENND1B (tGFP-tagged) - Human DENN/MADD domain containing 1B (DENND1B), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DENND1B
• Synonyms: C1ORF18; C1orf218; FAM31B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205585 representing NM_144977; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCAGCTCCACGGGAGGAGAAAAGATGGCCCCAACCTGTATTTTCGAATCCTGTGGTATTGTGGA; AATTCCCAGAGGACTTTGGAGACCAGGAAATACTACAGAGTGTGCCAAAGTTCTGTTTTCCCTTTGACGT; TGAAAGGGTGTCTCAGAATCAAGTTGGACAGCACTTTACCTTTGTACTGACAGACATTGAAAGTAAACAG; AGATTTGGATTCTGCAGACTGACGTCAGGAGGCACAATTTGTTTATGCATCCTTAGTTACCTTCCCTGGT; TTGAAGTGTATTACAAGCTTCTAAATACTCTTGCAGATTACTTGGCTAAGGAACTGGAAAATGATTTGAA; TGAAACTCTCAGATCACTGTATAACCACCCAGTACCAAAGGCAAATACTCCTGTAAATTTGAGTGTGCAT; TCCTACTTCATTGCCCCTGATGTAACTGGACTCCCAACAATACCCGAGAGTAGAAATCTTACAGAATATT; TTGTTGCCGTGGATGTGAACAACATGCTGCAGCTGTATGCCAGTATGCTGCATGAAAGGCGCATCGTGAT; TATCTCGAGCAAATTAAGCACTTTAACTGCCTGTATCCATGGATCAGCTGCTCTTCTATACCCAATGTAT; TGGCAACACATATACATCCCAGTGCTTCCTCCACACCTGCTGGACTACTGCTGTGCCCCAATGCCATACC; TGATTGGAATACACTCCAGCCTCATAGAGAGAGTGAAAAACAAATCATTGGAAGATGTTGTTATGTTAAA; TGTTGATACAAACACATTAGAATCACCATTTAGTGACTTGAACAACCTACCAAGTGATGTGGTCTCGGCC; TTGAAAAATAAACTGAAGAAGCAGTCTACAGCTACGGGTGATGGAGTAGCTAGGGCCTTTCTTAGAGCAC; AGGCTGCTTTGTTTGGATACTACAGAGATGCACTGAGATACAAACCTGGTGAGCCCATCACTTTCTGTGA; GGAGAGTTTTGTAAAGCACCGCTCAAGCGTGATGAAACAGTTCCTGGAAACTGCCATTAACCTCCAGCTT; TTTAAGCAGTTTATCGATGGTCGACTGGCAAAACTAAATGCAGGAAGGGGTTTCTCTGATGTATTTGAAG; AAGAGATCACTTCAGGTGGCTTTTGTGGAGGTAAAGACAAGTTACAATATGATTATCCATTTTCTCAA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205585 representing NM_144977; Red=Cloning site Green=Tags(s); ; MAAAPREEKRWPQPVFSNPVVLWKFPEDFGDQEILQSVPKFCFPFDVERVSQNQVGQHFTFVLTDIESKQ; RFGFCRLTSGGTICLCILSYLPWFEVYYKLLNTLADYLAKELENDLNETLRSLYNHPVPKANTPVNLSVH; SYFIAPDVTGLPTIPESRNLTEYFVAVDVNNMLQLYASMLHERRIVIISSKLSTLTACIHGSAALLYPMY; WQHIYIPVLPPHLLDYCCAPMPYLIGIHSSLIERVKNKSLEDVVMLNVDTNTLESPFSDLNNLPSDVVSA; LKNKLKKQSTATGDGVARAFLRAQAALFGYYRDALRYKPGEPITFCEESFVKHRSSVMKQFLETAINLQL; FKQFIDGRLAKLNAGRGFSDVFEEEITSGGFCGGKDKLQYDYPFSQ; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144977
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144977.2
• RefSeq Size: 2213 bp
• RefSeq ORF: 1281 bp
• Locus ID: 163486
• UniProt ID: Q6P3S1
• Cytogenetics: 1q31.3
• Protein Families: Transmembrane
• Summary: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]