HAT1 (NM_001033085) Human Untagged Clone

SKU SC324037

HAT1 (untagged)-Human histone acetyltransferase 1 (HAT1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: HAT1
• Synonyms: histone acetyltransferase 1; KAT1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001033085.1 GAGCGCGCGGGTTGATTCGTCCTTCCTCAGCCGCGGGTGATCGTAGCTCGGAAATGGCGG; GATTTGGTGCTATGGAGAAATTTTTGGTAGAATATAAGAGTGCAGTGGAGAAGAAACTGG; CAGAGTACAAATGTAACACCAACACAGCAATTGAACTAAAATTAGTGAAACTGCTTTTGG; TTACAAGGGTCTAAAGATCCTGTTATACTATATTGCTGGTAGCCTGTCAACAATGTTCCG; TGTTGAATATGCATCTAAAGTTGATGAGAACTTTGACTGTGTAGAGGCAGATGATGTTGA; GGGCAAAATTAGACAAATCATTCCACCTGGATTTTGCACAAACACGAATGATTTCCTTTC; TTTACTGGAAAAGGAAGTTGATTTCAAGCCATTCGGAACCTTACTTCATACCTACTCAGT; TCTCAGTCCAACAGGAGGAGAAAACTTTACCTTTCAGATATATAAGGCTGACATGACATG; TAGAGGCTTTCGAGAATATCATGAAAGGCTTCAGACCTTTTTGATGTGGTTTATTGAAAC; TGCTAGCTTTATTGACGTGGATGATGAAAGATGGCACTACTTTCTAGTATTTGAGAAGTA; TAATAAGGATGGAGCTACGCTCTTTGCGACCGTAGGCTACATGACAGTCTATAATTACTA; TGTGTACCCAGACAAAACCCGGCCACGTGTAAGTCAGATGCTGATTTTGACTCCATTTCA; AGGTCAAGGCCATGGTGCTCAACTTCTTGAAACAGTTCATAGATACTACACTGAATTTCC; TACAGTTCTTGATATTACAGCGGAAGATCCATCCAAAAGCTATGTGAAATTACGAGACTT; TGTGCTTGTGAAGCTTTGTCAAGATTTGCCCTGTTTTTCCCGGGAAAAATTAATGCAAGG; ATTCAATGAAGATATGGCGATAGAGGCACAACAGAAGTTCAAAATAAATAAGCAACACGC; TAGAAGGGTTTATGAAATTCTTCGACTACTGGTAACTGACATGAGTGATGCCGAACAATA; CAGAAGCTACAGACTGGATATTAAAAGAAGACTAATTAGCCCATATAAGAAAAAGCAGAG; AGATCTTGCTAAGATGAGAAAATGTCTCAGACCAGAAGAACTGACAAACCAGATGAACCA; AATAGAAATAAGCATGCAACATGAACAGCTGGAAGAGAGTTTTCAGGAACTAGTGGAAGA; TTACCGGCGTGTTATTGAACGACTTGCTCAAGAGTAAAGATTATACTGCTCTGTACAGGA; AGCTTGCAAATTTTCTGTACAATGTGCTGTGAAAAATCTGATGACTTTAATTTTAAAATC; TTGTGACATTTTGCTTATACTAAAAGTTATCTATCTTTAGTTGAATATTTTCTTTTGGAG; AGATTGTATATTTTAAAATACTGTTTAGAGTTTATGAGCATATATTGCATTTAAAGAAAG; ATAAAGCTTCTGAAATACTACTGCAATTGCTTCCCTTCTTAAACAGTATAATAAATGCTT; AGTTGTGATATGTTAATGTGTGATGATATGATTCTTAAATACTTACAATAAACCTCATTC; TTAAATACTTAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001033085
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001033085.1, NP_001028257.1
• RefSeq Size: 1596 bp
• RefSeq ORF: 1005 bp
• Locus ID: 8520
• Cytogenetics: 2q31.1
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]; Transcript Variant: This variant (2) lacks an alternate segment and uses a downstream start codon, compared to variant 1. Isoform b has a shorter N-terminus, compared to isoform a.