IL1RAP (NM_134470) Human Untagged Clone

SKU SC324029

IL1RAP (untagged)-Human interleukin 1 receptor accessory protein (IL1RAP), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: IL1RAP
• Synonyms: C3orf13; IL-1RAcP; IL1R3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_134470.2 AGACTGCCGGGATCCAGGTCTCCGGGGTCCGCTTTGGCCAGAGGCGCGGAAGGAAGCAGT; GCCCGGCGACACTGCACCCATCCCGGCTGCTTTTGCTGCGCCCTCTCAGCTTCCCAAGAA; AGGCATCGTCATGTGATCATCACCTAAGAACTAGAACATCAGCAGGCCCTAGAAGCCTCA; CTCTTGCCCCTCCCTTTAATATCTCAAAGGATGACACTTCTGTGGTGTGTAGTGAGTCTC; TACTTTTATGGAATCCTGCAAAGTGATGCCTCAGAACGCTGCGATGACTGGGGACTAGAC; ACCATGAGGCAAATCCAAGTGTTTGAAGATGAGCCAGCTCGCATCAAGTGCCCACTCTTT; GAACACTTCTTGAAATTCAACTACAGCACAGCCCATTCAGCTGGCCTTACTCTGATCTGG; TATTGGACTAGGCAGGACCGGGACCTTGAGGAGCCAATTAACTTCCGCCTCCCCGAGAAC; CGCATTAGTAAGGAGAAAGATGTGCTGTGGTTCCGGCCCACTCTCCTCAATGACACTGGC; AACTATACCTGCATGTTAAGGAACACTACATATTGCAGCAAAGTTGCATTTCCCTTGGAA; GTTGTTCAAAAAGACAGCTGTTTCAATTCCCCCATGAAACTCCCAGTGCATAAACTGTAT; ATAGAATATGGCATTCAGAGGATCACTTGTCCAAATGTAGATGGATATTTTCCTTCCAGT; GTCAAACCGACTATCACTTGGTATATGGGCTGTTATAAAATACAGAATTTTAATAATGTA; ATACCCGAAGGTATGAACTTGAGTTTCCTCATTGCCTTAATTTCAAATAATGGAAATTAC; ACATGTGTTGTTACATATCCAGAAAATGGACGTACGTTTCATCTCACCAGGACTCTGACT; GTAAAGGTAGTAGGCTCTCCAAAAAATGCAGTGCCCCCTGTGATCCATTCACCTAATGAT; CATGTGGTCTATGAGAAAGAACCAGGAGAGGAGCTACTCATTCCCTGTACGGTCTATTTT; AGTTTTCTGATGGATTCTCGCAATGAGGTTTGGTGGACCATTGATGGAAAAAAACCTGAT; GACATCACTATTGATGTCACCATTAACGAAAGTATAAGTCATAGTAGAACAGAAGATGAA; ACAAGAACTCAGATTTTGAGCATCAAGAAAGTTACCTCTGAGGATCTCAAGCGCAGCTAT; GTCTGTCATGCTAGAAGTGCCAAAGGCGAAGTTGCCAAAGCAGCCAAGGTGAAGCAGAAA; GGTAATAGATGCGGTCAGTGATGAATCTCTCAGCTCCAAATTAACATTGTGGTGAATAAG; GACAAAAGGAGAGATTGAGAACAAGAGAGCTCCAGCACCTAGCCTGACGGCATCTAACCC; ATAGTAATGAATCAAACTTAAATGAAAAATATGAAAGTTTTCATCTATGTAAGATACTCA; AAATATTGTTTCTGATATTGTTAGTACCGTAATGCCCAAATGTAGCTAAAAAAATCAACG; TGAGTACAGTGAGACACAATTTTGTGTCTGTACAATTATGAAAAAAAAAAAAAAAAAAAA; A
• Restriction Sites: ECoRI-NOT
• ACCN: NM_134470
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_134470.2, NP_608273.1
• RefSeq Size: 2063 bp
• RefSeq ORF: 1071 bp
• Locus ID: 3556
• UniProt ID: Q9NPH3
• Cytogenetics: 3q28
• Domains: ig, IG
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction
• Summary: This gene encodes a component of the interleukin 1 receptor complex, which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in membrane-bound and soluble isoforms differing in their C-terminus. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. [provided by RefSeq, Jul 2018]; Transcript Variant: This variant (2) differs in the 3' end of the coding region and 3' UTR, compared to variant 1. It encodes the soluble isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.