SHTN1 (NM_001127211) Human Untagged Clone

SKU SC323157

KIAA1598 (untagged)-Human KIAA1598 (KIAA1598), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SHTN1
• Synonyms: KIAA1598; shootin-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323157 representing NM_001127211.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAACAGCTCGGACGAAGAGAAGCAGCTGCAGCTCATTACCAGTCTGAAGGAGCAAGCAATAGGCGAA; TATGAAGACCTTAGAGCAGAGAACCAGAAAACAAAGGAGAAGTGTGACAAAATTAGGCAAGAACGAGAT; GAAGCCGTTAAAAAACTGGAAGAATTTCAGAAAATTTCTCACATGGTCATAGAGGAAGTTAATTTCATG; CAGAACCATCTTGAAATAGAGAAGACTTGTCGAGAAAGTGCTGAAGCTTTGGCAACAAAGCTAAATAAA; GAAAATAAAACGTTGAAAAGAATCAGCATGTTGTACATGGCCAAGCTGGGACCAGATGTAATAACTGAA; GAGATAAACATTGATGATGAAGATTCGACTACAGACACAGACGGTGCCGCCGAGACTTGTGTCTCAGTA; CAGTGTCAGAAGCAAATTAAAGAACTTCGAGATCAAATTGTATCTGTTCAGGAGGAAAAGAAGATTTTA; GCCATTGAGCTGGAAAATCTCAAGAGCAAACTCGTAGAAGTAATTGAAGAAGTAAATAAAGTTAAACAA; GAAAAGACTGTTTTAAATTCAGAAGTTCTTGAACAGAGAAAAGTCTTAGAAAAATGCAATAGAGTGTCC; ATGTTAGCTGTAGAAGAGTATGAGGAGATGCAAGTAAACCTGGAGCTGGAGAAGGACCTTCGAAAGAAA; GCAGAGTCATTTGCACAAGAGATGTTCATTGAGCAAAACAAGCTAAAGAGACAAAGCCACCTTCTGCTG; CAGAGCTCCATCCCTGATCAGCAGCTTTTGAAAGCTTTAGACGAAAATGCAAAACTCACCCAGCAACTT; GAAGAAGAGAGAATTCAGCATCAACAAAAGGTCAAAGAATTAGAAGAGCAACTAGAAAATGAAACACTC; CACAAAGAAATACACAACCTCAAACAGCAACTGGAGCTTCTAGAGGAAGATAAAAAGGAATTGGAATTG; AAATATCAGAATTCTGAAGAGAAAGCCAGAAATTTAAAGCACTCTGTTGATGAACTCCAGAAACGAGTG; AACCAGTCTGAGAATTCAGTACCTCCACCACCTCCTCCTCCACCACCACTTCCCCCTCCACCTCCCAAT; CCTATCCGATCCCTCATGTCCATGATCCGGAAACGATCCCACCCCAGTGGCAGTGGTGCTAAGAAAGAA; AAGGCAACTCAACCAGAAACAACTGAAGAAGTCACAGATCTAAAGAGGCAAGCAGTTGAAGAGATGATG; GATAGAATTAAAAAGGGAGTTCATCTTAGACCCGTTAATCAGACAGCCAGACCGAAGACAAAGCCAGAA; TCTTCGAAAGGCTGCGAAAGTGCAGTGGATGAACTAAAAGGAATACTGGGGACACTTAACAAATCCACT; AGTTCAAGAAGCTTAAAATCCCTTGACCCTGAAAACAGTGAAACTGAGTTAGAAAGGATTTTGCGTCGC; AGAAAGGTGACAGCAGAAGCAGATAGCAGTAGTCCAACTGGGATATTAGCCACCTCAGAGTCCAAATCC; ATGCCAGTGTTGGGTTCTGTATCCAGTGTAACAAAAACAGCCTTGAACAAGAAAACTCTGGAGGCAGAA; TTCAACAGCCCGTCCCCCCCAACACCTGAGCCAGGTGAAGGGCCCCGTAAATTGGAAGGATGCACAAGT; TCCAAGGTTACGTTTCAGCCTCCCAGTAGCATTGGATGCAGGAAAAAATACATTGACGGTGAAAAACAA; GCCGAACCAGTTGTAGTTTTAGATCCTGTTTCTACACATGAACCCCAAACCAAAGACCAGGTTGCTGAA; AAAGATCCAACTCAACACAAGGAGGATGAAGGCGAAATTCAACCAGAAAACAAAGAAGACAGCATTGAA; AACGTGAGAGAGACAGACAGCTCCAACTGCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127211
• Insert Size: 1896 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001127211.2
• RefSeq Size: 5361 bp
• RefSeq ORF: 1896 bp
• Locus ID: 57698
• UniProt ID: A0MZ66
• Cytogenetics: 10q25.3
• MW: 71.6 kDa
• Summary: Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth. Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth. Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. Plays also a role in regenerative neurite outgrowth. In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longest isoform (a, also known as shootin 1b).