Caspase 2 (CASP2) (NM_032982) Human Untagged Clone

SKU SC321316

CASP2 (untagged)-Human caspase 2, apoptosis-related cysteine peptidase (CASP2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Caspase 2
• Synonyms: CASP-2; ICH1; NEDD-2; NEDD2; PPP1R57
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032982 edited; GGAAAAGCGGGAAATGGCGGCGCCGAGCGCGGGGTCTTGGTCCACCTTCCAGCACAAGGA; GCTGATGGCCGCTGACAGGGGACGCAGGATATTGGGAGTGTGTGGCATGCATCCTCATCA; TCAGGAAACTCTAAAAAAGAACCGAGTGGTGCTAGCCAAACAGCTGTTGTTGAGCGAATT; ATTAGAACATCTTCTGGAGAAGGACATCATCACCTTGGAAATGAGGGAGCTCATCCAGGC; CAAAGTGGGCAGTTTCAGCCAGAATGTGGAACTCCTCAACTTGCTGCCTAAGAGGGGTCC; CCAAGCTTTTGATGCCTTCTGTGAAGCACTGAGGGAGACCAAGCAAGGCCACCTGGAGGA; TATGTTGCTCACCACCCTTTCTGGGCTTCAGCATGTACTCCCACCGTTGAGCTGTGACTA; CGACTTGAGTCTCCCTTTTCCGGTGTGTGAGTCCTGTCCCCTTTACAAGAAGCTCCGCCT; GTCGACAGATACTGTGGAACACTCCCTAGACAATAAAGATGGTCCTCTCTGCCTTCAGGT; GAAGCCTTGCACTCCTGAATTTTATCAAACAC:ACTTCCAGCTGGCATATAGGTTGCAGT; CTCGGCCTCGTGGCCTAGCACTGGTGTTGAGCAATGTGCACTTCACTGGAGAGAAAGAAC; TGGAATTTCGCTCTGGAGGGGATGTGGACCACAGTACTCTAGTCACCC:TCTTCAAGCTT; TTGGGCT:ATGA:CGTCCATGTTCTATGTGACCAGACTGCACAGGAAATGCAAGAGAAAC; TGCAGAATTTTGCACAGTTACCTGCACACCGAGTCACGGACTCCTGCATCGTGGCACTCC; TCTCGCATGGTGTGGAGGGCGCCATCTATGGTGTGGATGGGAAACTGCTCCAGCTCCAAG; AGGTTTTTCAGCTCTTTGACAACGCCAACTGCCCAAGCCTACAGAACAAACCAAAAATGT; TCTTCATCCAGGCCTGCCGTGGAGATGAGACTGATCGTGGGGTTGACCAACAAGATGGAA; AGAACCACGCAGGATCCCCTGGGTGCGAGGAGAGTGATGCCGGTAAAGAAAAGTTGCCGA; AGATGAGACTGCCCACGCGCTCAGACATGATATGCGGCTATGCCTGCCTCAAAGGGACTG; CCGCCATGCGGAACACCAAACGAGGTTCCTGGTACATCGAGGCTCTTGCTCAAGTGTTTT; CTGAGCGGGCTTGTGATATGCACGTGGCCGACATGCTGGTTAAGGTGAACGCACTTATCA; AGGATCGGGAAGGTTATGCTCCTGGCACAGAATTCCACCGGTGCAAGGAGATGTCTGAAT; ACTGCAGCACTCTGTGCCGCCACCTCTACCTGTTCCCAGGACACCCTCCCACATGATGTC; ACCTCCCCATCATCCACGCCAAGTGGAAGCCACTGGACCACAGGAGGTGTGATAGAGCCT; TTGATCTTCAGGATGCACGGTTTCTGTTCTGCCCCCTCAGGGATGTGGGAATCTCCCAGA; CTTGTTTCCTGTGCCCATCATCTCTGCCTTTGAGTGTGGGACTCCAGGCCAGCTCCTTTT; CTGTGAAGCCCTTTGCCTGTAGAGCCAGCCTTGGTTGGACCTATTGCCAGGAATGTTTCA; GCTGCAGTTGAAGAGCCTGACAAGTGAAGTTGTAAACACAGTGTGGTTATGGGGAGAGGG; CATATAAATTCCCCATATTTGTGTTCAGTTCCAGCTTTTGTAGATGGCACTTTAGTGATT; GCTTTTATTACATTAGTTAAGATGTCTGAGAGACCATCTCCTATCTTTTATTTCATTCAT; ATCCTCCGCCCTTTTTGTCCTAGAGTGAGAGTTTGGAAGGTGTCCAAATTTAATGTAGAC; ATTATCTTTTGGCTCTGAAGAAGCAAACATGACTAGAGACGCACCTTGCTGCAGTGTCCA; GAAGCGGCCTGTGCGTTCCCTTCAGTACTGCAGCGCCACCCAGTGGAAGGACACTCTTGG; CTCGTTTGGGCTCAAGGCACCGCAGCCTGTCAGCCAACATTGCCTTGCATTTGTACCTTA; TTGATCTTTGCCCATGGAAGTCTCAAAGATCTTTCGTTGGTTGTTTCTCTGAGCTTTGTT; ACTGAAATGAGCCTCGTGGGGAGCATCAGAGAAGGCCAGGAAGAATGGTGTGTTTCCCTA; GACTCTGTAACCACCTCTCTGTCTTTTTCCTTCCTGAGAAACGTCCATCTCTCTCCCTTA; CTATTCCCACTTTCATTCAATCAACCTGCACTTCATATCTAGATTTCTAGAAAAGCTTCC; TAGCTTATCTCCCTGCTTCATATCTCTCCCTTCTTTACCTTCATTTCATCCTGTTGGCTG; CTGCCACCAAATCTGTCTAGAATCCTGCTTTACAGGATCATGTAAATGCTCAAAGATGTA; ATGTAGTTCTTTGTTCCTGCTTTCTCTTTCAGTATTAAACTCTCCTTTGATATTATGTGG; CTTTTATTTCAGTGCCATACATGTTATTGTTTTCAACCTAGAAACCTTTATCCCTGCTTA; TCTGAAACTTCCCAACTTCCCTGATCTTTAAGACTTTTTTTTTTTTTTTTTTTTTTTTGA; GACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGGGCAGTGGCACGATCTCAGCTCACTGCA; AGCTCCAACTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTTCCAAGTAGCTGGGACTA; CAGGTGCCCGCCACCGTGCCCGGCTAATTTTTTTGTATTTTTAGTAGAGACAGGGTTTCA; CCATGTTAGCCGGGATGGTCTTGATCTCCTGACCTCATGATCCACCCACCTCAGCCTCCC; AAAGTGTTGGGATTACAGGCGTGAGCCACTGCGCCCGGGCAAGACCTTTTTTTAAAAAAA; AAAAAAAAAAAA;
• Restriction Sites: Please inquire
• ACCN: NM_032982
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032982.2, NP_116764.2
• RefSeq Size: 4145 bp
• RefSeq ORF: 1359 bp
• Locus ID: 835
• UniProt ID: P42575
• Cytogenetics: 7q34
• Domains: CARD, CASc, ICE_p10, ICE_p20
• Protein Families: Druggable Genome, Protease
• Summary: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]; Transcript Variant: This variant (1) encodes the longest isoform (1, also known as ICH-1L), which has been shown to function as a positive regulator of apoptosis (PMID:8087842). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.