GATA2 (NM_032638) Human Untagged Clone

CAT#: SC321007

GATA2 (untagged)-Human GATA binding protein 2 (GATA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GATA2
• Synonyms: DCML; IMD21; MONOMAC; NFE1B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032638.3 GAGCGCCAGGAAGGTAGCGAGGCCAGCGTCGCCCCGGGACTCGCTGCTCAAGTCTGTCTA
  TTGCCTGCCGCCACATCCATCCTAGCAGGGCCCCGTCGCCCACCAGGCGGACAAAAGCGG
  TCCGCTGAACACCATGCGGCCGCTCGGCGTGCCGCCCAGGCTCTGCTGGTGAGCGCCGCC
  ACCCCGCGCCCAGGTCCCGCGAGCCCGCCTGCCGCGCACCTCGCCCTGCTCCCAGCTCTA
  CTCCAGGCCCCGTCCGCCCGGGGGCGCCGCCCACCGCGCCTCGCTCGGGCCGTTGCCGTC
  TGCACCCAGACCCTGAGCCGCCGCCGCCGGCCATGGAGGTGGCGCCGGAGCAGCCGCGCT
  GGATGGCGCACCCGGCCGTGCTGAATGCGCAGCACCCCGACTCACACCACCCGGGCCTGG
  CGCACAACTACATGGAACCCGCGCAGCTGCTGCCTCCAGACGAGGTGGACGTCTTCTTCA
  ATCACCTCGACTCGCAGGGCAACCCCTACTATGCCAACCCCGCTCACGCGCGGGCGCGCG
  TCTCCTACAGCCCCGCGCACGCCCGCCTGACCGGAGGCCAGATGTGCCGCCCACACTTGT
  TGCACAGCCCGGGTTTGCCCTGGCTGGACGGGGGCAAAGCAGCCCTCTCTGCCGCTGCGG
  CCCACCACCACAACCCCTGGACCGTGAGCCCCTTCTCCAAGACGCCACTGCACCCCTCAG
  CTGCTGGAGGCCCTGGAGGCCCACTCTCTGTGTACCCAGGGGCTGGGGGTGGGAGCGGGG
  GAGGCAGCGGGAGCTCAGTGGCCTCCCTCACCCCTACAGCAGCCCACTCTGGCTCCCACC
  TTTTCGGCTTCCCACCCACGCCACCCAAAGAAGTGTCTCCTGACCCTAGCACCACGGGGG
  CTGCGTCTCCAGCCTCATCTTCCGCGGGGGGTAGTGCAGCCCGAGGAGAGGACAAGGACG
  GCGTCAAGTACCAGGTGTCACTGACGGAGAGCATGAAGATGGAAAGTGGCAGTCCCCTGC
  GCCCAGGCCTAGCTACTATGGGCACCCAGCCTGCTACACACCACCCCATCCCCACCTACC
  CCTCCTATGTGCCGGCGGCTGCCCACGACTACAGCAGCGGACTCTTCCACCCCGGAGGCT
  TCCTGGGGGGACCGGCCTCCAGCTTCACCCCTAAGCAGCGCAGCAAGGCTCGTTCCTGTT
  CAGAAGGCCGGGAGTGTGTCAACTGTGGGGCCACAGCCACCCCTCTCTGGCGGCGGGACG
  GCACCGGCCACTACCTGTGCAATGCCTGTGGCCTCTACCACAAGATGAATGGGCAGAACC
  GACCACTCATCAAGCCCAAGCGAAGACTGTCGGCCGCCAGAAGAGCCGGCACCTGTTGTG
  CAAATTGTCAGACGACAACCACCACCTTATGGCGCCGAAACGCCAACGGGGACCCTGTCT
  GCAACGCCTGTGGCCTCTACTACAAGCTGCACAATGTTAACAGGCCACTGACCATGAAGA
  AGGAAGGGATCCAGACTCGGAACCGGAAGATGTCCAACAAGTCCAAGAAGAGCAAGAAAG
  GGGCGGAGTGCTTCGAGGAGCTGTCAAAGTGCATGCAGGAGAAGTCATCCCCCTTCAGTG
  CAGCTGCCCTGGCTGGACACATGGCACCTGTGGGCCACCTCCCGCCCTTCAGCCACTCCG
  GACACATCCTGCCCACTCCGACGCCCATCCACCCCTCCTCCAGCCTCTCCTTCGGCCACC
  CCCACCCGTCCAGCATGGTGACCGCCATGGGCTAGGGAACAGATGGACGTCGAGGACCGG
  GCACTCCCGGGATGGGTGGACCAAACCCTTAGCAGCCCAGCATTTCCCGAAGGCCGACAC
  CACTCCTGCCAGCCCGGCTCGGCCCAGCACCCCCTCTCCTGGAGGGCGCCCAGCAGCCTG
  CCAGCAGTTACTGTGAATGTTCCCCACCGCTGAGAGGCTGCCTCCGCACCTGACTGCTGC
  CCAGGTGGGGTTTCCTGCATGGACAGTTGTTTGGAGAACAACAAGGACAACTTTATGTAG
  AGAAAAGGAGGGGACGGGACAGACGAAGGCAACCATTTTTAGAAGGAAAAAGGATTAGGC
  AAAAATAATTTATTTTGCTCTTGTTTCTAACAAGGACTTGGAGACTTGGTGGTCTGAGCT
  GTCCCAAGTCCTCCGGTTCTTCCTCGGGATTGGCGGGTCCACTTGCCAGGGCTCTGGGGG
  CAGATTTGTGGGGACCTCAGCCTGCACCCTCTTCTCTTCTGGCTTCCCTCTCTGAAATAG
  CCGAACTCCAGGCTGGGCTGAGCCAAAGCCAGAGTGGCCACGGCCCAGGGAGGGTGAGCT
  GGTGCCTGCTTTGACGGGCCAGGCCCTGGAGGGCAGAGACAATCACGGGCGGTCCTGCAC
  AGATTCCCAGGCCAGGGCTGGGTCACAGGAAGGAAACAACATTTTCTTGAAAGGGGAAAC
  GTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTGACTGTGTCCCCTTACT
  GAGCGCAAGCCACAGCCTGTCTTGTCAGGTGGACCCTGTAAATACATCCTTTTTCTGCTA
  ACCCTTCAACCCCCTCGCCTCCTACTCTGAGACAAAAGAAAAAATATTAAAAAAATGCAT
  AGGCTTAACTCGCTGATGAGTTAATTGTTTTATTTTTAAACTCTTTTTGGGTCCAGTTGA
  TTGTACGTAGCCACAGGAGCCCTGCTATGAAAGGAATAAAACCTACACACAAGGTTGGAG
  CTTTGCAATTCTTTTTGGAAAAGAGCTGGGATCCCACAGCCCTAGTATGAAAGCTGGGGG
  TGGGGAGGGGCCTTTGCTGCCCTTGGTTTCTGGGGGCTGGTTGGCATTTGCTGGCCTGGC
  AGGGGGTGAAGGCAGGAGTTGGGGGCAGGTCAGGACCAGGACCCAGGGAGAGGCTGTGTC
  CCTGCTGGGGTCTCAGGTCCAGCTTTACTGTGGCTGTCTGGATCCTTCCCAAGGTACAGC
  TGTATATAAACGTGTCCCGAGCTTAGATTCTGTATGCGGTGACGGCGGGGTGTGGTGGCC
  TGTGAGGGGCCCCTGGCCCAGGAGGAGGATTGTGCTGATGTAGTGACCAAGTGCAATATG
  GGCGGGCAGTCGCTGCAGGGAGCACCACGGCCAGAAGTAACTTATTTTGTACTAGTGTCC
  GCATAAGAAAAAGAATCGGCAGTATTTTCTGTTTTTATGTTTTATTTGGCTTGTTTTATT
  TTGGATTAGTGAACTAAGTTATTGTTAATTATGTACAACATTTATATATTGTCTGTAAAA
  AATGTATGCTATCCTCTTATTCCTTTAAAGTGAGTACTGTTAAGAATAATAAAATACTTT
  TTGTGAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_032638
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032638.3, NP_116027.2
• RefSeq Size: 3383 bp
• RefSeq ORF: 1443 bp
• Locus ID: 2624
• UniProt ID: P23769
• Cytogenetics: 3q21.3
• Domains: GATA
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1).