METTL14 (NM_020961) Human Untagged Clone

SKU SC319510

METTL14 (untagged)-Human methyltransferase like 14 (METTL14)

Product Data

• Type: Human Untagged Clone
• Target Symbol: METTL14
• Synonyms: hMETTL14
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020961.2 ATACTCTGTTCGTAAGCTCCCGGTGAATTTTGTTCCACAGACTCGGAAGAAAGGTTGGAT; AAGAGTTCACTGGAGATTGACAAGTACTCGGGATAGTGAAAAGCCGGAGTTGGAACATGG; ATAGCCGCTTGCAGGAGATCCGGGAGCGGCAGAAGTTACGGCGACAGCTCCTCGCGCAGC; AGTTGGGAGCTGAAAGTGCCGACAGCATTGGTGCCGTGTTAAATAGCAAAGATGAGCAGA; GAGAAATTGCTGAAACAAGAGAAACTTGCAGGGCTTCCTATGATACCTCTGCTCCAAATG; CAAAACGTAAGTATCTGGATGAAGGAGAGACAGATGAAGACAAAATGGAAGAATATAAGG; ATGAACTAGAAATGCAACAGGATGAAGAAAATTTGCCATATGAAGAAGAGATTTACAAAG; ATTCTAGTACTTTTCTTAAGGGAACACAGAGCTTAAATCCCCATAATGATTACTGCCAAC; ATTTTGTAGACACTGGACATAGACCTCAGAATTTCATCAGGGATGTAGGTTTAGCTGACA; GATTTGAAGAATATCCTAAACTGAGGGAGCTCATCAGGCTAAAGGATGAGTTAATAGCTA; AATCTAACACTCCTCCCATGTACTTACAAGCCGATATAGAAGCCTTTGACATCAGAGAAC; TAACACCCAAATTTGATGTGATTCTTCTGGAACCCCCTTTAGAAGAATATTACAGAGAAA; CTGGCATCACTGCTAATGAAAAATGCTGGACTTGGGATGATATTATGAAGTTAGAAATTG; ATGAGATTGCAGCACCTCGATCATTTATTTTTCTCTGGTGTGGTTCTGGGGAGGGGTTGG; ACCTTGGAAGAGTGTGTTTACGAAAATGGGGTTACAGAAGATGTGAAGATATTTGTTGGA; TTAAAACCAATAAAAACAATCCTGGGAAGACTAAGACTTTAGATCCAAAGGCTGTCTTTC; AGAGAACAAAGGAACACTGCCTCATGGGGATCAAAGGAACTGTGAAGCGTAGCACAGACG; GGGACTTCATTCATGCTAATGTTGACATTGACTTAATTATCACAGAAGAACCTGAAATTG; GCAATATAGAAAAACCTGTAGAAATTTTTCATATAATTGAGCATTTTTGTCTTGGTAGAA; GACGCCTTCATCTATTTGGAAGAGATAGTACAATTCGACCAGGCTGGCTCACAGTTGGAC; CAACGCTTACAAATAGCAACTACAATGCAGAAACATATGCATCCTATTTCAGTGCTCCTA; ATTCCTACTTGACTGGTTGTACAGAAGAAATTGAGAGACTTCGACCAAAATCGCCTCCTC; CCAAATCTAAATCTGACCGAGGAGGTGGAGCTCCCAGAGGTGGAGGAAGAGGTGGAACTT; CTGCTGGCCGTGGACGAGAAAGAAATAGATCTAACTTCCGAGGAGAAAGAGGTGGCTTTA; GAGGGGGCCGTGGAGGAGCACACAGAGGTGGCTTTCCACCTCGATAATTGTTGAAGACAT; TGAACCTATTCATCCTCCTCTAACCTTCTTTATTGTAATTAAATTTCAAGTGGGAGACTT; AACTTTAGAACTCACTTCCAGCTTGCACTTTGCTTTAATTTCTCTGAGCTGCAAGAATGT; CTTAGCGAGCCTTGCTTGCAGTTGTCACACACACTGTCCGGTTTTTTTCAGGATAAATGA; ATGATTCTGCCTTTTGTTATGTGCGTGAACAGAATGGAACAACTCAAGTAGCTTCATCTT; CAGAGACTGAATTTATTCTGATAGACTTCAGCTAATTACAAAGGATTTTGCTAATTTTTG; GGAATAAATAATGGAAAAAGATCCAGTCTGTGGTATCATGCTAGTGCTGACAGGGCCTTG; ATAGAATAGAGTTGGAAAAGATGGTAAGCTTTTGTCAGGGTTTTAACATTTTCTTGATGA; AACAATAAAAAGAGGTAAGCTTTTTTCTTCTTTTTTTTTAAGTTTTAAATAAACTCAGAT; ATAATTTGAATACTGAAGAAATTAAGAGACTTTGAACAAAATCTCTTCCCAAATCTAAAT; TTGATAGGGGAGGTGGAGATTCCAGGGGTGGGTGAAAGAAGAGATAGAACTTAGCAGGCA; GACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_020961
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020961.2, NP_066012.1
• RefSeq Size: 2138 bp
• RefSeq ORF: 1371 bp
• Locus ID: 57721
• UniProt ID: Q9HCE5
• Cytogenetics: 4q26
• Domains: MT-A70
• Summary: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:29348140, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194, PubMed:29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).[UniProtKB/Swiss-Prot Function]