METTL14 (NM_020961) Human Tagged ORF Clone

SKU RG200925

METTL14 (tGFP-tagged) - Human methyltransferase like 14 (METTL14)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: METTL14
• Synonyms: hMETTL14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200925 representing NM_020961; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATAGCCGCTTGCAGGAGATCCGGGAGCGGCAGAAGTTACGGCGACAGCTCCTCGCGCAGCAGTTGG; GAGCTGAAAGTGCCGACAGCATTGGTGCCGTGTTAAATAGCAAAGATGAGCAGAGAGAAATTGCTGAAAC; AAGAGAAACTTGCAGGGCTTCCTATGATACCTCTGCTCCAAATGCAAAACGTAAGTATCTGGATGAAGGA; GAGACAGATGAAGACAAAATGGAAGAATATAAGGATGAACTAGAAATGCAACAGGATGAAGAAAATTTGC; CATATGAAGAAGAGATTTACAAAGATTCTAGTACTTTTCTTAAGGGAACACAGAGCTTAAATCCCCATAA; TGATTACTGCCAACATTTTGTAGACACTGGACATAGACCTCAGAATTTCATCAGGGATGTAGGTTTAGCT; GACAGATTTGAAGAATATCCTAAACTGAGGGAGCTCATCAGGCTAAAGGATGAGTTAATAGCTAAATCTA; ACACTCCTCCCATGTACTTACAAGCCGATATAGAAGCCTTTGACATCAGAGAACTAACACCCAAATTTGA; TGTGATTCTTCTGGAACCCCCTTTAGAAGAATATTACAGAGAAACTGGCATCACTGCTAATGAAAAATGC; TGGACTTGGGATGATATTATGAAGTTAGAAATTGATGAGATTGCAGCACCTCGATCATTTATTTTTCTCT; GGTGTGGTTCTGGGGAGGGGTTGGACCTTGGAAGAGTGTGTTTACGAAAATGGGGTTACAGAAGATGTGA; AGATATTTGTTGGATTAAAACCAATAAAAACAATCCTGGGAAGACTAAGACTTTAGATCCAAAGGCTGTC; TTTCAGAGAACAAAGGAACACTGCCTCATGGGGATCAAAGGAACTGTGAAGCGTAGCACAGACGGGGACT; TCATTCATGCTAATGTTGACATTGACTTAATTATCACAGAAGAACCTGAAATTGGCAATATAGAAAAACC; TGTAGAAATTTTTCATATAATTGAGCATTTTTGTCTTGGTAGAAGACGCCTTCATCTATTTGGAAGAGAT; AGTACAATTCGACCAGGCTGGCTCACAGTTGGACCAACGCTTACAAATAGCAACTACAATGCAGAAACAT; ATGCATCCTATTTCAGTGCTCCTAATTCCTACTTGACTGGTTGTACAGAAGAAATTGAGAGACTTCGACC; AAAATCGCCTCCTCCCAAATCTAAATCTGACCGAGGAGGTGGAGCTCCCAGAGGTGGAGGAAGAGGTGGA; ACTTCTGCTGGCCGTGGACGAGAAAGAAATAGATCTAACTTCCGAGGAGAAAGAGGTGGCTTTAGAGGGG; GCCGTGGAGGAGCACACAGAGGTGGCTTTCCACCTCGA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200925 representing NM_020961; Red=Cloning site Green=Tags(s); ; MDSRLQEIRERQKLRRQLLAQQLGAESADSIGAVLNSKDEQREIAETRETCRASYDTSAPNAKRKYLDEG; ETDEDKMEEYKDELEMQQDEENLPYEEEIYKDSSTFLKGTQSLNPHNDYCQHFVDTGHRPQNFIRDVGLA; DRFEEYPKLRELIRLKDELIAKSNTPPMYLQADIEAFDIRELTPKFDVILLEPPLEEYYRETGITANEKC; WTWDDIMKLEIDEIAAPRSFIFLWCGSGEGLDLGRVCLRKWGYRRCEDICWIKTNKNNPGKTKTLDPKAV; FQRTKEHCLMGIKGTVKRSTDGDFIHANVDIDLIITEEPEIGNIEKPVEIFHIIEHFCLGRRRLHLFGRD; STIRPGWLTVGPTLTNSNYNAETYASYFSAPNSYLTGCTEEIERLRPKSPPPKSKSDRGGGAPRGGGRGG; TSAGRGRERNRSNFRGERGGFRGGRGGAHRGGFPPR; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020961
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020961.4
• RefSeq Size: 2138 bp
• RefSeq ORF: 1371 bp
• Locus ID: 57721
• UniProt ID: Q9HCE5
• Cytogenetics: 4q26
• Domains: MT-A70
• Summary: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:29348140, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194, PubMed:29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).[UniProtKB/Swiss-Prot Function]