MCM2 (NM_004526) Human Untagged Clone

CAT#: SC319263

MCM2 (untagged)-Human minichromosome maintenance complex component 2 (MCM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM2
• Synonyms: BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; MITOTIN
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004526.2 CGCGAAACCTGGTTGTTGCTGTAGTGGCGGAGAGGATCGTGGTACTGCTATGGCGGAATC
  ATCGGAATCCTTCACCATGGCATCCAGCCCGGCCCAGCGTCGGCGAGGCAATGATCCTCT
  CACCTCCAGCCCTGGCCGAAGCTCCCGGCGTACTGATGCCCTCACCTCCAGCCCTGGCCG
  TGACCTTCCACCATTTGAGGATGAGTCCGAGGGGCTCCTAGGCACAGAGGGGCCCCTGGA
  GGAAGAAGAGGATGGAGAGGAGCTCATTGGAGATGGCATGGAAAGGGACTACCGCGCCAT
  CCCAGAGCTGGACGCCTATGAGGCCGAGGGACTGGCTCTGGATGATGAGGACGTAGAGGA
  GCTGACGGCCAGTCAGAGGGAGGCAGCAGAGCGGGCCATGCGGCAGCGTGACCGGGAGGC
  TGGCCGGGGCCTGGGCCGCATGCGCCGTGGGCTCCTGTATGACAGCGATGAGGAGGACGA
  GGAGCGCCCTGCCCGCAAGCGCCGCCAGGTGGAGCGGGCCACGGAGGACGGCGAGGAGGA
  CGAGGAGATGATTGAGAGCATCGAGAACCTGGAGGATCTCAAAGGCCACTCTGTGCGCGA
  GTGGGTGAGCATGGCGGGCCCCCGGCTGGAGATCCACCACCGCTTCAAGAACTTCCTGCG
  CACTCACGTCGACAGCCACGGCCACAACGTCTTCAAGGAGCGCATCAGCGACATGTGCAA
  AGAGAACCGTGAGAGCCTGGTGGTGAACTATGAGGACTTGGCAGCCAGGGAGCACGTGCT
  GGCCTACTTCCTGCCTGAGGCACCGGCGGAGCTGCTGCAGATCTTTGATGAGGCTGCCCT
  GGAGGTGGTACTGGCCATGTACCCCAAGTACGACCGCATCACCAACCACATCCATGTCCG
  CATCTCCCACCTGCCTCTGGTGGAGGAGCTGCGCTCGCTGAGGCAGCTGCATCTGAACCA
  GCTGATCCGCACCAGTGGGGTGGTGACCAGCTGCACTGGCGTCCTGCCCCAGCTCAGCAT
  GGTCAAGTACAACTGCAACAAGTGCAATTTCGTCCTGGGTCCTTTCTGCCAGTCCCAGAA
  CCAGGAGGTGAAACCAGGCTCCTGTCCTGAGTGCCAGTCGGCCGGCCCCTTTGAGGTCAA
  CATGGAGGAGACCATCTATCAGAACTACCAGCGTATCCGAATCCAGGAGAGTCCAGGCAA
  AGTGGCGGCTGGCCGGCTGCCCCGCTCCAAGGACGCCATTCTCCTCGCAGATCTGGTGGA
  CAGCTGCAAGCCAGGAGACGAGATAGAGCTGACTGGCATCTATCACAACAACTATGATGG
  CTCCCTCAACACTGCCAATGGCTTCCCTGTCTTTGCCACTGTCATCCTAGCCAACCACGT
  GGCCAAGAAGGACAACAAGGTTGCTGTAGGGGAACTGACCGATGAAGATGTGAAGATGAT
  CACTAGCCTCTCCAAGGATCAGCAGATCGGAGAGAAGATCTTTGCCAGCATTGCTCCTTC
  CATCTATGGTCATGAAGACATCAAGAGAGGCCTGGCTCTGGCCCTGTTCGGAGGGGAGCC
  CAAAAACCCAGGTGGCAAGCACAAGGTACGTGGTGATATCAACGTGCTCTTGTGCGGAGA
  CCCTGGCACAGCGAAGTCGCAGTTTCTCAAGTATATTGAGAAAGTGTCCAGCCGAGCCAT
  CTTCACCACTGGCCAGGGGGCGTCGGCTGTGGGCCTCACGGCGTATGTCCAGCGGCACCC
  TGTCAGCAGGGAGTGGACCTTGGAGGCTGGGGCCCTGGTTCTGGCTGACCGAGGAGTGTG
  TCTCATTGATGAATTTGACAAGATGAATGACCAGGACAGAACCAGCATCCATGAGGCCAT
  GGAGCAACAGAGCATCTCCATCTCGAAGGCTGGCATCGTCACCTCCCTGCAGGCTCGCTG
  CACGGTCATTGCTGCCGCCAACCCCATAGGAGGGCGCTACGACCCCTCGCTGACTTTCTC
  TGAGAACGTGGACCTCACAGAGCCCATCATCTCACGCTTTGACATCCTGTGTGTGGTGAG
  GGACACCGTGGACCCAGTCCAGGACGAGATGCTGGCCCGCTTCGTGGTGGGCAGCCACGT
  CAGACACCACCCCAGCAACAAGGAGGAGGAGGGGCTGGCCAATGGCAGCGCTGCTGAGCC
  CGCCATGCCCAACACGTATGGCGTGGAGCCCCTGCCCCAGGAGGTCCTGAAGAAGTACAT
  CATCTACGCCAAGGAGAGGGTCCACCCGAAGCTCAACCAGATGGACCAGGACAAGGTGGC
  CAAGATGTACAGTGACCTGAGGAAAGAATCTATGGCGACAGGCAGCATCCCCATTACGGT
  GCGGCACATCGAGTCCATGATCCGCATGGCGGAGGCCCACGCGCGCATCCATCTGCGGGA
  CTATGTGATCGAAGACGACGTCAACATGGCCATCCGCGTGATGCTGGAGAGCTTCATAGA
  CACACAGAAGTTCAGCGTCATGCGCAGCATGCGCAAGACTTTTGCCCGCTACCTTTCATT
  CCGGCGTGACAACAATGAGCTGTTGCTCTTCATACTGAAGCAGTTAGTGGCAGAGCAGGT
  GACATATCAGCGCAACCGCTTTGGGGCCCAGCAGGACACTATTGAGGTCCCTGAGAAGGA
  CTTGGTGGATAAGGCTCGTCAGATCAACATCCACAACCTCTCTGCATTTTATGACAGTGA
  GCTCTTCAGGATGAACAAGTTCAGCCACGACCTGAAAAGGAAAATGATCCTGCAGCAGTT
  CTGAGGCCCTATGCCATCCATAAGGATTCCTTGGGATTCTGGTTTGGGGTGGTCAGTGCC
  CTCTGTGCTTTATGGACACAAAACCAGAGCACTTGATGAACTCGGGGTACTAGGGTCAGG
  GCTTATAGCAGGATGTCTGGCTGCACCTGGCATGACTGTTTGTTTCTCCAAGCCTGCTTT
  GTGCTTCTCACCTTTGGGTGGGATGCCTTGCCAGTGTGTCTTACTTGGTTGCTGAACATC
  TTGCCACCTCCGAGTGCTTTGTCTCCACTCAGTACCTTGGATCAGAGCTGCTGAGTTCAG
  GATGCCTGCGTGTGGTTTAGGTGTTAGCCTTCTTACATGGATGTCAGGAGAGCTGCTGCC
  CTCTTGGCGTGAGTTGCGTATTCAGGCTGCTTTTGCTGCCTTTGGCCAGAGAGCTGGTTG
  AAGATGTTTGTAATCGTTTTCAGTCTCCTGCAGGTTTCTGTGCCCCTGTGGTGGAAGAGG
  GCACGACAGTGCCAGCGCAGCGTTCTGGGCTCCTCAGTCGCAGGGGTGGGATGTGAGTCA
  TGCGGATTATCCACTCGCCACAGTTATCAGCTGCCATTGCTCCCTGTCTGTTTCCCCACT
  CTCTTATTTGTGCATTCGGTTTGGTTTCTGTAGTTTTAATTTTTAATAAAGTTGAATAAA
  ATATAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004526
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004526.2, NP_004517.2
• RefSeq Size: 3453 bp
• RefSeq ORF: 2715 bp
• Locus ID: 4171
• UniProt ID: P49736
• Cytogenetics: 3q21.3
• Domains: MCM
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
  Transcript Variant: This variant (1) encodes a functional protein.