pCMV6-AC Mammalian Expression Vector

CAT#: PS100020

pCMV6-AC, mammalian vector with non-tagged expression, 10ug

Product Images

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Specifications

Product Data

• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Features:
  • This vector is essentially the same as the pCMV6-Entry vector, except for the absence of the C-terminal Myc-DDK tag.
  • ORF cloned into this vector at SgfI and RsrII sites will express the ORF-encoded protein plus two additional amino acids at the C-terminus, T and R.
  • The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • XL39_R Primer can be used to sequence the target sequence after cloning.
• Download Vector Sequence:
• Schematic of the multiple cloning sites: