pCMV6-AC Mammalian Expression Vector

CAT#: PS100020

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pCMV6-AC, mammalian vector with non-tagged expression, 10ug

Datasheet
 Citations (32)

USD 570.00

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Size
    • 10 ug

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Specifications

Product Data
E. coli Selection Ampicillin
Cell Selection Neomycin
Features
  • This vector is essentially the same as the pCMV6-Entry vector, except for the absence of the C-terminal Myc-DDK tag.
  • ORF cloned into this vector at SgfI and RsrII sites will express the ORF-encoded protein plus two additional amino acids at the C-terminus, T and R.
  • The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.
  • Download full vector sequence
Schematic of the multiple cloning sites

Citations (32)

The use of this Vectors has been cited in the following citations:
Role of Isocitrate Dehydrogenase 2 on DNA Hydroxymethylation in Human Airway Smooth Muscle Cells ,Yeung, BH;Huang, J;An, SS;Solway, J;Mitzner, W;Tang, WY;, Am. J. Respir. Cell Mol. Biol. 2020 ,PubMed ID 32150688 [pCMV6-AC]
CYLD is a causative gene for frontotemporal dementia - amyotrophic lateral sclerosis ,Dobson-Stone, C;Hallupp, M;Shahheydari, H;Ragagnin, AMG;Chatterton, Z;Carew-Jones, F;Shepherd, CE;Stefen, H;Paric, E;Fath, T;Thompson, EM;Blumbergs, P;Short, CL;Field, CD;Panegyres, PK;Hecker, J;Nicholson, G;Shaw, AD;Fullerton, JM;Luty, AA;Schofield, PR;Brooks, WS;Rajan, N;Bennett, MF;Bahlo, M;Landers, JE;Piguet, O;Hodges, JR;Halliday, GM;Topp, SD;Smith, BN;Shaw, CE;McCann, E;Fifita, JA;Williams, KL;Atkin, JD;Blair, IP;Kwok, JB;, Brain 2020 ,PubMed ID 32185393 [pCMV6-AC]
Mutant p53 induces SIRT3/MnSOD axis to moderate ROS production in melanoma cells ,Torrens-Mas, M;Cordani, M;Mullappilly, N;Pacchiana, R;Riganti, C;Palmieri, M;Pons, DG;Roca, P;Oliver, J;Donadelli, M;, Arch. Biochem. Biophys. 2019 ,PubMed ID 31812668 [pCMV6-AC]
Clinical Significance of SERPINA1 Gene and Its Encoded Alpha1-antitrypsin Protein in NSCLC ,Ercetin, E;Richtmann, S;Delgado, BM;Gomez-Mariano, G;Wrenger, S;Korenbaum, E;Liu, B;DeLuca, D;Kühnel, MP;Jonigk, D;Yuskaeva, K;Warth, A;Muley, T;Winter, H;Meister, M;Welte, T;Janciauskiene, S;Schneider, MA;, Cancers (Basel) 2019 ,PubMed ID 31487965 [pCMV6-AC]
Endocytosis of very low-density lipoprotein particles: an unexpected mechanism for lipid acquisition by breast cancer cells ,Lupien, LE;Bloch, K;Dehairs, J;Feng, WW;Davis, WL;, bioRxiv 2019 [pCMV6-AC]
Exon 2 Skipping Eliminates Gamma-Glutamyl Carboxylase Activity, Indicating a Partial Splicing Defect in a Patient with Vitamin K Clotting Factor Deficiency ,Rishavy, MA;Hallgren, KW;Zhang, H;Runge, KW;Berkner, KL;, J. Thromb. Haemost. 2019 ,PubMed ID 31009158 [pCMV6-AC]
LEADeR role of miR-205 host gene as long noncoding RNA in prostate basal cell differentiation ,Profumo, V;Forte, B;Percio, S;Rotundo, F;Doldi, V;Ferrari, E;Fenderico, N;Dugo, M;Romagnoli, D;Benelli, M;Valdagni, R;Dolfini, D;Zaffaroni, N;Gandellini, P;, Nat Commun 2019 ,PubMed ID 30659180 [pCMV6-AC]
Fab-conjugated PLGA nanoparticles effectively target cancer cells expressing human CD44v6 ,Kennedy, PJ;Sousa, F;Ferreira, D;Pereira, C;Nestor, M;Oliveira, C;Granja, PL;Sarmento, B;, Acta Biomater 2018 ,PubMed ID 30267881 [pCMV6-AC]
Lamin A/C might be involved in the EMT signalling pathway ,Zuo, L;Zhao, H;Yang, R;Wang, L;Ma, H;Xu, X;Zhou, P;Kong, L;, Gene 2018 ,PubMed ID 29665450 [pCMV6-AC]
Traditional Chinese medicine, Fuzheng Kang‑Ai decoction, inhibits metastasis of lung cancer cells through the STAT3/MMP9 pathway ,Li, L;Wang, S;Yang, X;Long, S;Xiao, S;Wu, W;Hann, SS;, Mol Med Rep 2017 ,PubMed ID 28677797 [pCMV6-AC]
Identification of keratin 19-positive cancer stem cells associating human hepatocellular carcinoma using CYFRA 21-1 ,Kawai, T;Yasuchika, K;Ishii, T;Katayama, H;Yoshitoshi, EY;Ogiso, S;Minami, T;Miyauchi, Y;Kojima, H;Yamaoka, R;Kita, S;Yasuda, K;Sasaki, N;Fukumitsu, K;Hatano, E;Uemoto, S;, Cancer Med 2017 ,PubMed ID 28965351 [pCMV6-AC]
The enhancement of combination of berberine and metformin in inhibition of DNMT1 gene expression through interplay of SP1 and PDPK1 ,Zheng, F;Wu, J;Tang, Q;Xiao, Q;Wu, W;Hann, SS;, J. Cell. Mol. Med. 2017 ,PubMed ID 28840963 [pCMV6-AC]
Activation of Phenyl 4-(2-Oxo-3-alkylimidazolidin-1-yl)benzenesulfonates Prodrugs by CYP1A1 as New Antimitotics Targeting Breast Cancer Cells ,Fortin, S;Charest-Morin, X;Turcotte, V;Lauvaux, C;Lacroix, J;Côté, MF;Gobeil, S;C-Gaudreault, R;, J. Med. Chem. 2017 ,PubMed ID 28535350 [pCMV6-AC]
EZH2 inhibition suppresses endometrial cancer progression via miR-361/Twist axis ,Ihira, K;Dong, P;Xiong, Y;Watari, H;Konno, Y;Hanley, SJ;Noguchi, M;Hirata, N;Suizu, F;Yamada, T;Kudo, M;Sakuragi, N;, Oncotarget 2017 ,PubMed ID 28088786 [pCMV6-AC]
Lowered Expression of Tumor Suppressor Candidate MYO1C Stimulates Cell Proliferation, Suppresses Cell Adhesion and Activates AKT ,Visuttijai, K;Pettersson, J;Mehrbani Azar, Y;van den Bout, I;Örndal, C;Marcickiewicz, J;Nilsson, S;Hörnquist, M;Olsson, B;Ejeskär, K;Behboudi, A;, PLoS ONE 2016 ,PubMed ID 27716847 [pCMV6-AC]
Chinese herbal medicine Fuzheng Kang-Ai decoction sensitized the effect of gefitinib on inhibition of human lung cancer cells through inactivating PI3-K/Akt-mediated ,Li, L;Wang, SM;Zheng, F;Wu, WY;Hann, SS;, Journal of Ethnopharmacology 2016 ,PubMed ID 27989877
Identification of keratin19-positive cancer stem cells associating human hepatocellular carcinoma using 18F-fluorodeoxyglucose positron emission tomography ,Kawai, T;Yasuchika, K;Seo, S;Higashi, T;Ishii, T;Miyauchi, Y;Kojima, H;Yamaoka, R;Katayama, H;Yoshitoshi, EY;Ogiso, S;Kita, S;Yasuda, K;Fukumitsu, K;Nakamoto, Y;Hatano, E;Uemoto, S;, Clin. Cancer Res. 2016 ,PubMed ID 27663597
Influence of the APOE genotype on hepatic stress response: Studies in APOE targeted replacement mice and human liver cells ,Dose, J;Nebel, A;Piegholdt, S;Rimbach, G;Huebbe, P;, Free Radic. Biol. Med. Apr 2016 ,PubMed ID 27130033
Inactivation of PI3-K/Akt and reduction of SP1 and p65 expression increase the effect of solamargine on suppressing EP4 expression in human lung cancer cells ,Chen, Y;Tang, Q;Wu, J;Zheng, F;Yang, L;Hann, SS;, J. Exp. Clin. Cancer Res. Dec 2015 ,PubMed ID 26689593 [PS100020]
Se-Allylselenocysteine induces autophagy by modulating the AMPK/mTOR signaling pathway and epigenetic regulation of PCDH17 in human colorectal adenocarcinoma cells ,Wu, JC;Wang, FZ;Tsai, ML;Lo, CY;Badmaev4, V;Ho, CT;Wang, YJ;Pan, MH;, Mol Nutr Food Res Sep 2015 ,PubMed ID 26395119 [PS100020]
Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes ,Meier, D;Lopez, M;Franken, P;Fontana, A;, PLoS ONE Sep 2015 ,PubMed ID 26361389 [PS100020]
Multiple conformations are a conserved and regulatory feature of the RB1 5' UTR ,Kutchko, KM;Sanders, W;Ziehr, B;Phillips, G;Solem, A;Halvorsen, M;Weeks, KM;Moorman, N;Laederach, A;, RNA May 2015 ,PubMed ID 25999316 [PS100020]
The Transcription Factor C-Myc Suppresses MiR-23b and MiR-27b Transcription during Fetal Distress and Increases the Sensitivity of Neurons to Hypoxia-Induced Apoptosis ,Chen, Q;Zhang, F;Wang, Y;Liu, Z;Sun, A;Zen, K;Zhang, CY;Zhang, Q;, PLoS ONE Mar 2015 ,PubMed ID 25781629 [PS100020]
Angiopoietin-like protein 2 induces androgen-independent and malignant behavior in human prostate cancer cells ,Sato, R;Yamasaki, M;Hirai, K;Matsubara, T;Nomura, T;Sato, F;Mimata, H;, Oncol. Rep. Jan 2015 ,PubMed ID 25370833 [PS100020]
UBE2L3 Polymorphism Amplifies NF-?B Activation and Promotes Plasma Cell Development, Linking Linear Ubiquitination to Multiple Autoimmune Diseases ,Lewis, MJ;Vyse, S;Shields, AM;Boeltz, S;Gordon, PA;Spector, TD;Lehner, PJ;Walczak, H;Vyse, TJ;, Am. J. Hum. Genet. Jan 2015 ,PubMed ID 25640675 [PS100020]
ADAM8 as a drug target in pancreatic cancer ,Schlomann, U;Koller, G;Conrad, C;Ferdous, T;Golfi, P;Garcia, AM;Höfling, S;Parsons, M;Costa, P;Soper, R;Bossard, M;Hagemann, T;Roshani, R;Sewald, N;Ketchem, RR;Moss, ML;Rasmussen, FH;Miller, MA;Lauffenburger, DA;Tuveson, DA;Nimsky, C;Bartsch, JW;, Nat Commun Jan 2015 ,PubMed ID 25629724 [PS100020]
Intestinal neuroendocrine cells and goblet cells are mediators of IL-17A-amplified epithelial IL-17C production in human inflammatory bowel disease ,Friedrich, M;Diegelmann, J;Schauber, J;Auernhammer, CJ;Brand, S;, Mucosal Immunol Dec 2014 ,PubMed ID 25492478 [PS100020]
Involvement of vacuolar H(+) -ATPase in killing of human melanoma cells by the sphingosine kinase analogue FTY720 ,Tay, KH;Liu, X;Chi, M;Jin, L;Jiang, CC;Guo, ST;Verrills, NM;Tseng, HY;Zhang, XD;, Pigment Cell Melanoma Res Oct 2014 ,PubMed ID 25358761 [BCRT101]
Japanese encephalitis virus vaccine candidates generated by chimerization with dengue virus type 4 ,Gromowski, GD;Firestone, CY;Hanson, CT;Whitehead, SS;, Vaccine. March 2014 ,PubMed ID 24699473 [pCMV6-AC]
P2Y2 receptor represses IL-6 expression by valve interstitial cells through Akt: Implication for calcific aortic valve disease ,El Husseini, D;Boulanger, MC;Mahmut, A;Bouchareb, R;Laflamme, MH;Fournier, D;Pibarot, P;Bossé, Y;Mathieu, P;, J. Mol. Cell. Cardiol. Mar 2014 ,PubMed ID 24631773 [PCMV6-AC]
Identifikation und Charakterisierung neuer frühdiagnostischer Biomarker für das rezidivierende Prostatakarzinom ,Geisler, C;, Thesis Jan 2014 [TR30007]
A congenital disorder of deglycosylation: Biochemical characterization of N-glycanase 1 deficiencyin patient fibroblasts ,He, P;Grotzke, JE;Ng, BG;Gunel, M;Jafar-Nejad, H;, Thesis May 2015 [PS100020]
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