MCM2 (NM_004526) Human Tagged ORF Clone

CAT#: RG202506

MCM2 (tGFP-tagged) - Human minichromosome maintenance complex component 2 (MCM2)

Product Images

Circular map for RG202506

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MCM2
• Synonyms: BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; MITOTIN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202506 representing NM_004526
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAATCATCGGAATCCTTCACCATGGCATCCAGCCCGGCCCAGCGTCGGCGAGGCAATGATCCTC
  TCACCTCCAGCCCTGGCCGAAGCTCCCGGCGTACTGATGCCCTCACCTCCAGCCCTGGCCGTGACCTTCC
  ACCATTTGAGGATGAGTCCGAGGGGCTCCTAGGCACAGAGGGGCCCCTGGAGGAAGAAGAGGATGGAGAG
  GAGCTCATTGGAGATGGCATGGAAAGGGACTACCGCGCCATCCCAGAGCTGGACGCCTATGAGGCCGAGG
  GACTGGCTCTGGATGATGAGGACGTAGAGGAGCTGACGGCCAGTCAGAGGGAGGCAGCAGAGCGGGCCAT
  GCGGCAGCGTGACCGGGAGGCTGGCCGGGGCCTGGGCCGCATGCGCCGTGGGCTCCTGTATGACAGCGAT
  GAGGAGGACGAGGAGCGCCCTGCCCGCAAGCGCCGCCAGGTGGAGCGGGCCACGGAGGACGGCGAGGAGG
  ACGAGGAGATGATTGAGAGCATCGAGAACCTGGAGGATCTCAAAGGCCACTCTGTGCGCGAGTGGGTGAG
  CATGGCGGGCCCCCGGCTGGAGATCCACCACCGCTTCAAGAACTTCCTGCGCACTCACGTCGACAGCCAC
  GGCCACAACGTCTTCAAGGAGCGCATCAGCGACATGTGCAAAGAGAACCGTGAGAGCCTGGTGGTGAACT
  ATGAGGACTTGGCAGCCAGGGAGCACGTGCTGGCCTACTTCCTGCCTGAGGCACCGGCGGAGCTGCTGCA
  GATCTTTGATGAGGCTGCCCTGGAGGTGGTACTGGCCATGTACCCCAAGTACGACCGCATCACCAACCAC
  ATCCATGTCCGCATCTCCCACCTGCCTCTGGTGGAGGAGCTGCGCTCGCTGAGGCAGCTGCATCTGAACC
  AGCTGATCCGCACCAGTGGGGTGGTGACCAGCTGCACTGGCGTCCTGCCCCAGCTCAGCATGGTCAAGTA
  CAACTGCAACAAGTGCAATTTCGTCCTGGGTCCTTTCTGCCAGTCCCAGAACCAGGAGGTGAAACCAGGC
  TCCTGTCCTGAGTGCCAGTCGGCCGGCCCCTTTGAGGTCAACATGGAGGAGACCATCTATCAGAACTACC
  AGCGTATCCGAATCCAGGAGAGTCCAGGCAAAGTGGCGGCTGGCCGGCTGCCCCGCTCCAAGGACGCCAT
  TCTCCTCGCAGATCTGGTGGACAGCTGCAAGCCAGGAGACGAGATAGAGCTGACTGGCATCTATCACAAC
  AACTATGATGGCTCCCTCAACACTGCCAATGGCTTCCCTGTCTTTGCCACTGTCATCCTAGCCAACCACG
  TGGCCAAGAAGGACAACAAGGTTGCTGTAGGGGAACTGACCGATGAAGATGTGAAGATGATCACTAGCCT
  CTCCAAGGATCAGCAGATCGGAGAGAAGATCTTTGCCAGCATTGCTCCTTCCATCTATGGTCATGAAGAC
  ATCAAGAGAGGCCTGGCTCTGGCCCTGTTCGGAGGGGAGCCCAAAAACCCAGGTGGCAAGCACAAGGTAC
  GTGGTGATATCAACGTGCTCTTGTGCGGAGACCCTGGCACAGCGAAGTCGCAGTTTCTCAAGTATATTGA
  GAAAGTGTCCAGCCGAGCCATCTTCACCACTGGCCAGGGGGCGTCGGCTGTGGGCCTCACGGCGTATGTC
  CAGCGGCACCCTGTCAGCAGGGAGTGGACCTTGGAGGCTGGGGCCCTGGTTCTGGCTGACCGAGGAGTGT
  GTCTCATTGATGAATTTGACAAGATGAATGACCAGGACAGAACCAGCATCCATGAGGCCATGGAGCAACA
  GAGCATCTCCATCTCGAAGGCTGGCATCGTCACCTCCCTGCAGGCTCGCTGCACGGTCATTGCTGCCGCC
  AACCCCATAGGAGGGCGCTACGACCCCTCGCTGACTTTCTCTGAGAACGTGGACCTCACAGAGCCCATCA
  TCTCACGCTTTGACATCCTGTGTGTGGTGAGGGACACCGTGGACCCAGTCCAGGACGAGATGCTGGCCCG
  CTTCGTGGTGGGCAGCCACGTCAGACACCACCCCAGCAACAAGGAGGAGGAGGGGCTGGCCAATGGCAGC
  GCTGCTGAGCCCGCCATGCCCAACACGTATGGCGTGGAGCCCCTGCCCCAGGAGGTCCTGAAGAAGTACA
  TCATCTACGCCAAGGAGAGGGTCCACCCGAAGCTCAACCAGATGGACCAGGACAAGGTGGCCAAGATGTA
  CAGTGACCTGAGGAAAGAATCTATGGCGACAGGCAGCATCCCCATTACGGTGCGGCACATCGAGTCCATG
  ATCCGCATGGCGGAGGCCCACGCGCGCATCCATCTGCGGGACTATGTGATCGAAGACGACGTCAACATGG
  CCATCCGCGTGATGCTGGAGAGCTTCATAGACACACAGAAGTTCAGCGTCATGCGCAGCATGCGCAAGAC
  TTTTGCCCGCTACCTTTCATTCCGGCGTGACAACAATGAGCTGTTGCTCTTCATACTGAAGCAGTTAGTG
  GCAGAGCAGGTGACATATCAGCGCAACCGCTTTGGGGCCCAGCAGGACACTATTGAGGTCCCTGAGAAGG
  ACTTGGTGGATAAGGCTCGTCAGATCAACATCCACAACCTCTCTGCATTTTATGACAGTGAGCTCTTCAG
  GATGAACAAGTTCAGCCACGACCTGAAAAGGAAAATGATCCTGCAGCAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202506 representing NM_004526
  Red=Cloning site Green=Tags(s)
  
  MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGE
  ELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSD
  EEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSH
  GHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNH
  IHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPG
  SCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHN
  NYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHED
  IKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYV
  QRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAA
  NPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGS
  AAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESM
  IRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLV
  AEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004526
• ORF Size: 2712 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004526.2, NP_004517.2
• RefSeq Size: 3453 bp
• RefSeq ORF: 2715 bp
• Locus ID: 4171
• UniProt ID: P49736
• Cytogenetics: 3q21.3
• Domains: MCM
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]