WHAMM (NM_001080435) Human Untagged Clone

SKU SC315836

WHAMM (untagged)-Human WAS protein homolog associated with actin, golgi membranes and microtubules (WHAMM)

Product Data

• Type: Human Untagged Clone
• Target Symbol: WHAMM
• Synonyms: WHAMM1; WHDC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315836 representing NM_001080435.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGGACGAGCAGCCTGACAGCCTGGAGGGCTGGGTGCCGGTCCGGGAGGGCCTCTTCGCCGAGCCC; GAGAGGCACCGGCTGCGCTTCCTGGTGGCCTGGAACGGCGCGGAGGGCAAGTTCGCTGTGACTTGTCAC; GACCGTACCGCGCAGCAGCGGCGGCTGCGCGAGGGGGCCCGGTTGGGGCCCGAGCCCGAGCCCAAGCCT; GAGGCCGCCGTCTCCCCGTCCAGCTGGGCCGGCCTGCTCTCGGCCGCGGGGCTCCGCGGCGCGCACCGG; CAGTTGGCGGCGCTGTGGCCGCCTCTGGAGCGCTGCTTCCCGCGGCTGCCGCCGGAGCTGGACGTGGGC; GGCGGCGGGGCCTGGGGTCTGGGGCTCGGGCTGTGGGCGCTGCTGTGGCCGACGCGCGCGGGTCCCGGC; GAGGCGGCGCTGCAGGAGCTGTGCGGGCAGCTGGAACGCTATCTGGGCGCGGCGGCCGACGGCTGCGGC; GGCGCCACAGTGCGCGACGCACTCTTCCCGGCTGAGGGCGGCGCGGCCGACTGCGAAAGCCCGCGCGAG; TTCCGGGAGCGGGCCTTGCGCGCGCGGTGGGTCGAGGCGGACGCGCGGCTGCGCCAGGTTATTCAAGGA; CACGGAAAAGCCAACACCATGGTAGCATTAATGAACGTTTACCAAGAGGAAGATGAAGCATACCAGGAA; TTGGTTACCGTGGCAACCATGTTCTTCCAGTACTTATTGCAGCCATTTAGGGCTATGCGAGAAGTTGCA; ACTTTATGTAAGCTTGATATTTTGAAGTCTTTGGATGAGGATGACCTAGGTCCTAGAAGGGTAGTTGCC; CTGGAGAAAGAAGCTGAAGAATGGACCAGACGGGCTGAAGAAGCTGTCGTCTCTATTCAGGATATCACA; GTGAATTATTTTAAGGAGACAGTAAAAGCATTAGCAGGAATGCAGAAAGAAATGGAACAGGATGCGAAG; AGATTTGGTCAGGCTGCCTGGGCCACAGCAATTCCCAGGTTGGAAAAACTTCAGCTAATGCTAGCTCGA; GAGACTCTGCAACTCATGAGAGCGAAAGAGTTGTGTTTAAATCACAAAAGAGCTGAAATTCAGGGAAAG; ATGGAAGATCTTCCAGAACAAGAAAAAAATACAAATGTTGTAGATGAATTAGAAATACAATTTTATGAA; ATTCAATTAGAACTATATGAAGTTAAATTTGAGATATTAAAAAACGAAGAAATACTGCTTACTACACAG; TTGGACTCTCTTAAAAGACTTATAAAAGAAAAACAAGATGAAGTTGTCTATTACGATCCATGTGAAAAT; CCAGAGGAACTTAAAGTCATTGACTGTGTGGTGGGGCTGCAGGATGATAAGAATTTGGAAGTGAAAGAA; CTCAGAAGGCAGTGCCAGCAGCTGGAGTCTAAACGGGGCAGGATCTGTGCCAAAAGAGCCTCTCTCCGG; AGTAGAAAGGATCAGTGCAAAGAAAATCATCGGTTCAGATTGCAACAGGCTGAAGAAAGCATAAGATAC; TCTCGTCAGCATCACAGTATTCAGATGAAAAGAGACAAGATAAAAGAAGAGGAGCAAAAGAAAAAAGAA; TGGATCAACCAAGAACGTCAAAAAACACTCCAACGATTGAGATCATTTAAAGATAAACGCCTAGCTCAA; TCTGTCCGAAACACCTCTGGCTCAGAACCTGTGGCTCCAAACCTGCCAAGTGATCTTTCCCAGCAGATG; TGCTTGCCAGCTTCCCACGCGGTGTCAGTAATTCACCCGTCCTCTAGGAAAACTAGAGGTGTTCCCCTA; TCGGAAGCTGGTAATGTGAAAAGCCCCAAGTGTCAAAACTGTCATGGAAATATCCCTGTCCAGGTTTTT; GTTCCAGTTGGTGATCAAACACATTCCAAATCCAGTGAGGAATTGTCACTGCCACCACCTCCTCCTCCT; CCACCACCACCACCGCCGCCACCGCCGCCCCCACCCCCTCCTCTCCGTGCTCTGTCCTCATCCTCTCAA; GCTGCAACTCATCAGAACTTAGGCTTCCGGGCTCCAGTGAAAGATGACCAGCCACGTCCTCTAGTGTGC; GAATCACCTGCTGAGCGACCACGTGACTCCTTGGAAAGTTTTTCATGTCCAGGATCTATGGATGAAGTG; TTGGCCTCCTTAAGGCATGGCAGAGCTCCTCTCCGGAAGGTGGAAGTGCCGGCGGTGCGCCCTCCCCAC; GCCTCAATCAATGAGCACATTCTGGCTGCCATAAGGCAAGGGGTCAAACTGAAGAAAGTTCACCCTGAT; CTTGGCCCAAACCCCAGCAGCAAACCAACCAGCAACAGACGCACCAGTGACCTTGAGAGGAGCATCAAG; GCTGCGCTCCAGAGAATCAAGAGGGTGTCTGCTGACTCTGAGGAGGACAGTGATGAGCAGGACCCTGGC; CAGTGGGATGGTTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080435
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001080435.2
• RefSeq Size: 3859 bp
• RefSeq ORF: 2430 bp
• Locus ID: 123720
• UniProt ID: Q8TF30
• Cytogenetics: 15q25.2
• MW: 90.9 kDa
• Summary: This gene encodes a protein that plays a role in actin nucleation, Golgi membrane association and microtubule binding. The encoded protein is a nucleation-promoting factor that regulates the Actin-related protein 2/3 complex. The activated complex initiates growth of new actin filaments by binding to existing actin filaments. The encoded protein also functions in regulation of transport from the endoplasmic reticulum to the Golgi complex and in maintenance of the Golgi complex near the centrosome. Four pseudogenes of this gene are present on the same arm of chromosome 15 as this gene. [provided by RefSeq, Aug 2013]