TMEM16K (ANO10) (NM_018075) Human Untagged Clone

SKU SC315603

ANO10 (untagged)-Human anoctamin 10 (ANO10), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TMEM16K
• Synonyms: SCAR10; TMEM16K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315603 representing NM_018075.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAAAGTGACCTTATCAGCTTTGGATACTTCTGAGAGTTCTTTCACACCTTTGGTGGTCATAGAACTT; GCTCAGGATGTCAAAGAAGAAACCAAAGAATGGCTGAAAAACAGAATTATAGCTAAAAAAAAAGATGGA; GGTGCCCAGTTGTTGTTTAGACCATTGTTAAATAAATATGAACAAGAAACACTAGAAAATCAGAACTTA; TATCTTGTTGGTGCCTCCAAGATTAGAATGTTACTAGGGGCAGAAGCAGTGGGATTGGTAAAAGAGTGC; AATGATAACACCATGAGAGCCTTCACATACAGAACCAGACAGAACTTCAAAGGTTTTGATGATAACAAT; GATGATTTCCTGACAATGGCAGAATGTCAATTCATTATCAAACATGAACTTGAAAATCTTAGAGCTAAA; GATGAAAAAATGATCCCTGGTTACCCTCAGGCAAAGTTGTATCCAGGAAAATCATTGTTGAGAAGATTG; CTCACGTCTGGCATCGTGATTCAGGTGTTTCCACTGCATGACAGTGAAGCCCTGAAGAAGCTTGAGGAC; ACCTGGTACACTCGGTTTGCTTTGAAGTATCAGCCCATAGACAGTATTCGTGGCTACTTTGGGGAAACA; ATTGCTCTGTACTTTGGATTTTTGGAGTATTTCACTTTTGCATTAATCCCCATGGCTGTCATTGGGTTA; CCTTACTACTTGTTTGTGTGGGAAGACTATGACAAGTACGTGATCTTTGCCTCGTTCAACCTCATCTGG; TCCACGGTGATTCTGGAACTGTGGAAGCGTGGCTGTGCCAACATGACCTACAGGTGGGGGACACTGCTC; ATGAAGAGAAAGTTTGAGGAGCCCCGGCCAGGATTTCATGGTGTCTTGGGTATCAATTCCATCACTGGG; AAGGAGGAGCCTCTGTACCCCAGCTACAAGAGACAGTTGCGCATTTACCTGGTCTCCCTGCCATTCGTG; TGCCTCTGCCTCTATTTCTCACTGTATGTCATGATGATTTACTTCGACATGGAGGTTTGGGCCTTGGGT; CTACATGAGAACAGCGGGTCTGAGTGGACCAGTGTCCTGTTGTATGTGCCCAGCATCATCTATGCCATT; GTGATTGAGATCATGAATCGTCTCTATCGATATGCTGCCGAGTTTTTAACTTCATGGGAGAATCACAGA; TTGGAATCTGCCTATCAGAACCATCTAATTCTGAAAGTTTTAGTGTTCAACTTCCTCAATTGCTTTGCC; TCACTCTTCTATATTGCCTTTGTCTTGAAAGATATGAAGCTTTTGCGCCAGAGCTTGGCCACTCTCCTA; ATTACCTCCCAGATCCTCAACCAAATTATGGAATCTTTTCTTCCTTATTGGCTCCAAAGGAAGCATGGT; GTGCGGGTGAAGAGGAAGGTGCAGGCTTTAAAGGCAGACATTGATGCTACATTATATGAACAAGTCATC; CTGGAAAAAGAAATGGGAACTTATTTGGGCACCTTTGATGATTACTTGGAGTTATTCCTGCAGTTTGGT; TATGTGAGCCTTTTCTCCTGTGTTTACCCATTAGCAGCTGCCTTTGCTGTGTTAAATAACTTCACTGAA; GTAAATTCAGATGCCTTAAAAATGTGCAGGGTCTTCAAACGTCCATTCTCAGAACCTTCAGCCAATATT; GGTGTGTGGCAGTTGGCTTTTGAAACGATGAGTGTTATATCTGTGGTCACTAACTGTGCGCTGATTGGA; ATGTCACCACAAGTGAATGCAGTCTTTCCAGAATCAAAAGCAGACCTCATTTTGATTGTAGTAGCAGTG; GAGCACGCACTCCTGGCTTTAAAGTTTATACTTGCATTTGCCATACCTGATAAGCCACGGCATATCCAG; ATGAAACTAGCCAGACTGGAATTTGAGTCTTTGGAGGCACTCAAGCAGCAGCAAATGAAGCTCGTGACC; GAGAACCTGAAGGAGGAACCAATGGAAAGCGGGAAGGAGAAGGCAACCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_018075
• Insert Size: 1983 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018075.3
• RefSeq Size: 2743 bp
• RefSeq ORF: 1983 bp
• Locus ID: 55129
• UniProt ID: Q9NW15
• Cytogenetics: 3p22.1-p21.33
• Domains: DUF590
• Protein Families: Transmembrane
• MW: 76.3 kDa
• Summary: The transmembrane protein encoded by this gene belongs to the anoctamin family of calcium-activated chloride channels, also known as the transmembrane 16 family. The encoded protein contains eight transmembrane domains with cytosolic N- and C-termini. Defects in this gene may cause autosomal recessive spinocerebellar ataxia-10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]; Transcript Variant: This variant (1) encodes isoform (1). Variants 1 and 11 encode the same isoform.