DHX35 (NM_021931) Human Untagged Clone

SKU SC314469

DHX35 (untagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 35 (DHX35), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DHX35
• Synonyms: C20orf15; DDX35; KAIA0875
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314469 representing NM_021931.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCTGCGCCCGTGGGACCGGTGAAGTTCTGGCGACCCGGTACAGAGGGGCCAGGTGTAAGCATCTCT; GAAGAGAGACAAAGTCTGGCTGAAAACTCTGGGACAACGGTTGTTTACAACCCTTATGCTGCCCTTTCC; ATAGAGCAGCAGAGGCAGAAGCTGCCGGTATTCAAGCTTAGGAATCATATTTTATACTTGATAGAAAAT; TATCAGACAGTGGTGATTGTTGGTGAAACAGGATGTGGGAAGAGCACACAGATTCCTCAGTACCTTGCA; GAAGCCGGCTGGACAGCTGAAGGAAGAGTGGTAGGAGTGACCCAGCCTCGAAGAGTGGCTGCTGTTACA; GTTGCAGGGAGAGTAGCTGAAGAAAGGGGTGCAGTGCTGGGCCACGAGGTGGGCTACTGCATCCGCTTT; GATGACTGCACCGACCAGCTGGCCACGAGAATTAAGTTTCTTACTGATGGAATGCTGGTCAGGGAAATG; ATGGTTGATCCGTTGTTAACAAAATATAGTGTCATCATGCTGGATGAAGCCCACGAGAGGACCTTGTAC; ACTGACATTGCCATTGGCTTGCTAAAAAAGATTCAGAAAAAGCGAGGGGATCTTCGATTGATTGTAGCT; TCAGCCACTCTGGATGCAGACAAATTCCGGGATTTCTTTAATCAAAATGAAACCAGTGATCCAGCAAGG; GATACATGTGTGATCCTTACAGTGGAAGGGAGAACATTTCCGGTGGATATCTTTTATCTACAAAGTCCT; GTTCCAGATTATATCAAATCAACTGTCGAAACTGTGGTGAAAATTCACCAGACAGAGGGAGACGGAGAC; GTTTTAGCATTTCTTACTGGCCAGGAAGAGGTAGAAACTGTTGTGTCGATGCTCATCGAGCAGGCTCGA; GCACTAGCTCGCACTGGGATGAAGAGACACCTCCGAGTTCTCCCCATGTATGCAGGACTGCCTTCCTTT; GAGCAAATGAAAGTGTTTGAAAGGGTGTCACGCAGTGTCAGAAAGGTGATAGTGGCCACCAATGTGGCA; GAAACCTCTATCACAATCAGCGGCATTGTGTATGTGATCGACTGTGGCTTTGTGAAACTCCGAGCCTAC; AATCCCAGGACAGCTATTGAATGCTTGGTGGTGGTGCCAGTCTCCCAGGCATCAGCTAATCAGCGAGCA; GGACGTGGTGGTCGTAGTCGCTCGGGAAAATGTTATCGCCTTTATACAGAGGAAGCCTTTGACAAGTTG; CCTCAGTCTACGGTTCCTGAGATGCAGCGTAGTAATTTGGCACCTGTCATCCTGCAGCTGAAAGCACTA; GGAATTGACAATGTCCTCAGGTTCCACTTCATGTCGCCCCCTCCAGCACAGTCGATGGTTCAAGCCTTG; GAGTTACTGTATGCTCTGGGAGGTCTGGACAAAGACTGTCGCCTAACTGAACCGCTTGGCATGAGAATT; GCAGAGTTTCCTTTGAATCCCATGTTTGCCAAAATGCTGCTTGAATCAGGAAACTTCGGCTGTTCTCAG; GAAATTCTAAGCATCGCTGCCATGATGCAGATCCAGAATATCTTTGTGGTCCCCCCAAACCAGAAGTCT; CACGCAATTCGAGTGCACCGTAAATTTGCTGTGGAGGAGGGCGACCACCTCACTATGCTCAATATATAT; GAAGCATTTATCAAACACAATAAGGACTCTAAATGGTGTCAGGAACATTTCCTGAATTACAAGGGTCTT; GTCAGAGCTGCGACTGTAAGAGAACAATTGAAAAAGCTTCTTGTCAAGTTTCAAGTGCCCAGGAAGTCT; AGTGAAGGTGACCCGGATCTGGTTCTGAGGTGCATTGTCTCCGGCTTCTTCGCCAATGCAGCGAGGTTT; CATTCTACTGGAGCTTATAGGACCATCCGTGATGACCATGAGCTGCACATACACCCTGCGTCAGTCCTC; TATGCAGAGAAGCCGCCTCGCTGGGTCATCTATAACGAAGTTATACAGACCTCCAAGTACTACATGAGA; GATGTGACTGCCATTGAATCGGCCTGGCTGTTGGAGCTGGCTCCACACTTTTATCAACAAGGAACGCAC; CTGTCTCTGAAAGCCAAAAGGGCCAAGGTCCAGGACCCGTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_021931
• Insert Size: 2112 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021931.3
• RefSeq Size: 3336 bp
• RefSeq ORF: 2112 bp
• Locus ID: 60625
• UniProt ID: Q9H5Z1
• Cytogenetics: 20q11.23-q12
• Domains: DEAD, HA2, helicase_C
• MW: 78.9 kDa
• Summary: DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2010]; Transcript Variant: This variant (1) encodes the longer isoform (1).