TMEM24 (C2CD2L) (NM_014807) Human Untagged Clone

SKU SC310158

C2CD2L (untagged)-Human C2CD2-like (C2CD2L)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TMEM24
• Synonyms: DLNB23; TMEM24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310158 representing NM_014807.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGATCCGGGCTGGGGGCAGCGGGACGTGGGCTGGGCGGCCTTGCTGATCCTCTTCGCCGCCTCGCTG; CTCACGGTGTTCGCCTGGCTGCTGCAATATGCCCGGGGCTTGTGGCTGGCGCGGGCCCGCGGGGACCGG; GGCCCGGGACCCGCCTTAGCCGGGGAACCCGCGGGTTCCCTGCGGGAGCTGGGCGTGTGGCGCTCGCTG; CTGCGGCTGCGGGCGACTCGGGCTGGCGCCGCCGAGGAGCCAGGAGTCCGGGGCCTCCTGGCGTCACTC; TTCGCCTTCAAGTCTTTCCGGGAGAACTGGCAGCGGGCTTGGGTGCGAGCGCTGAACGAGCAGGCCTGC; AGAAACGGGAGCTCCATCCAAATCGCCTTTGAGGAGGTGCCCCAACTCCCACCCAGAGCCAGCATCAGT; CATGTGACCTGCGTAGACCAATCTGAGCATACCATGGTGCTGCGTTGCCAGCTCTCTGCTGAGGAGGTG; CGGTTCCCAGTCTCTGTGACCCAGCAGTCCCCCGCTGCCGTCTCCATGGAGACCTACCACGTCACTCTG; ACACTGCCACCAACACAGTTGGAAGTCAACCTGGAGGAAATCCCTGGTGAGGGGCTGCTCATATCCTGG; GCCTTCACTGATCGCCCAGATCTCAGCCTAACGGTGCTTCCCAAGCTTCAGGCCAGGGAGAGAGGTGAA; GAACAAGTGGAGCTCTCCACAATTGAGGAACTGATCAAGGATGCCATAGTCAGCACCCAGCCAGCCATG; ATGGTCAACCTCAGGGCTTGCTCTGCCCCAGGAGGCCTGGTACCCAGTGAGAAGCCACCCATGATGCCC; CAGGCTCAGCCAGCCATCCCCAGACCTAACCGGTTATTCCTACGGCAGCTTCGGGCATCTCACTTGGGA; AATGAGCTGGAAGGCACCGAGGAACTGTGCTGTGTAGCTGAACTCGACAACCCCATGCAGCAGAAGTGG; ACCAAGCCCGCGAGGGCTGGATCCGAGGTGGAGTGGACAGAAGACCTGGCACTGGATCTGGGCCCCCAG; AGCCGGGAGCTGACCCTCAAAGTGCTGAGGAGCAGCAGCTGTGGAGACACCGAACTCCTAGGCCAGGCC; ACACTGCCTGTGGGCTCCCCCTCCAGACCACTGTCTCGAAGACAGTTGTGCCCACTCACCCCAGGGCCA; GGGAAAGCCCTGGGACCAGCAGCCACCATGGCAGTGGAGCTTCACTATGAGGAGGGCTCTCCCCGGAAC; CTGGGTACTCCCACCTCCTCCACTCCACGCCCCAGCATCACACCTACCAAGAAGATTGAGCTTGACCGG; ACCATCATGCCCGATGGCACCATTGTCACCACAGTCACCACTGTCCAGTCCCGGCCCCGTATAGACGGC; AAATTAGACTCCCCCTCCCGCTCCCCGTCCAAGGTGGAGGTGACCGAGAAGACGACAACTGTGCTGAGT; GAGAGCAGTGGCCCCAGCAATACCTCCCATAGCAGCAGCCCAGGGGACAGCCACCTTTCCAACGGCTTG; GACCCTGTAGCAGAGACAGCGATTCGCCAGCTGACAGAGCCCAGTGGGCGGGTGGCCAAGAAGACACCC; ACCAAGCGCAGCACTCTCATCATCTCTGGTGTTTCCAAGGTGCCCATTGCTCAGGACGAGTTGGCGCTA; TCCCTGGGCTATGCGGCATCCCTGGAAGCCTCAGTGCAGGATGATGCAGGGACCAGCGGAGGCCCCTCT; TCACCTCCCTCAGACCCACCAGCCATGTCTCCAGGACCGCTAGATGCCCTCTCTAGTCCCACAAGTGTC; CAGGAAGCAGACGAGACAACCCGTTCGGATATTTCTGAGAGGCCATCTGTGGATGATATTGAGTCGGAA; ACGGGGTCCACTGGTGCCCTGGAGACCCGCAGCCTCAAGGATCACAAAGTGAGTTTCCTGCGCAGCGGC; ACTAAGCTCATCTTCCGCCGGAGGCCTAGGCAGAAGGAAGCTGGCCTGAGCCAATCACACGATGACCTC; TCCAACGCAACGGCCACGCCCAGTGTCCGAAAGAAGGCCGGCAGCTTTTCTCGCCGCCTTATCAAGCGC; TTTTCCTTCAAATCCAAACCCAAGGCCAATGGTAACCCCAGCCCCCAGCTCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_014807
• Insert Size: 2124 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014807.4
• RefSeq Size: 3402 bp
• RefSeq ORF: 2124 bp
• Locus ID: 9854
• UniProt ID: O14523
• Cytogenetics: 11q23.3
• Protein Families: Transmembrane
• MW: 76.2 kDa
• Summary: Lipid-binding protein that transports phosphatidylinositol, the precursor of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), from its site of synthesis in the endoplasmic reticulum to the cell membrane (PubMed:28209843). It thereby maintains the pool of cell membrane phosphoinositides, which are degraded during phospholipase C (PLC) signaling (PubMed:28209843). Plays a key role in the coordination of Ca(2+) and phosphoinositide signaling: localizes to sites of contact between the endoplasmic reticulum and the cell membrane, where it tethers the two bilayers (PubMed:28209843). In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane, abolishing phosphatidylinositol transport to the cell membrane (PubMed:28209843). Positively regulates insulin secretion in response to glucose: phosphatidylinositol transfer to the cell membrane allows replenishment of PI(4,5)P2 pools and calcium channel opening, priming a new population of insulin granules (PubMed:28209843).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).