TMEM24 (C2CD2L) (NM_014807) Human Tagged ORF Clone

SKU RG203922

C2CD2L (tGFP-tagged) - Human C2CD2-like (C2CD2L)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TMEM24
• Synonyms: DLNB23; TMEM24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203922 representing NM_014807; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATCCGGGCTGGGGGCAGCGGGACGTGGGCTGGGCGGCCTTGCTGATCCTCTTCGCCGCCTCGCTGC; TCACGGTGTTCGCCTGGCTGCTGCAATATGCCCGGGGCTTGTGGCTGGCGCGGGCCCGCGGGGACCGGGG; CCCGGGACCCGCCTTAGCCGGGGAACCCGCGGGTTCCCTGCGGGAGCTGGGCGTGTGGCGCTCGCTGCTG; CGGCTGCGGGCGACTCGGGCTGGCGCCGCCGAGGAGCCAGGAGTCCGGGGCCTCCTGGCGTCACTCTTCG; CCTTCAAGTCTTTCCGGGAGAACTGGCAGCGGGCTTGGGTGCGAGCGCTGAACGAGCAGGCCTGCAGAAA; CGGGAGCTCCATCCAAATCGCCTTTGAGGAGGTGCCCCAACTCCCACCCAGAGCCAGCATCAGTCATGTG; ACCTGCGTAGACCAATCTGAGCATACCATGGTGCTGCGTTGCCAGCTCTCTGCTGAGGAGGTGCGGTTCC; CAGTCTCTGTGACCCAGCAGTCCCCCGCTGCCGTCTCCATGGAGACCTACCACGTCACTCTGACACTGCC; ACCAACACAGTTGGAAGTCAACCTGGAGGAAATCCCTGGTGAGGGGCTGCTCATATCCTGGGCCTTCACT; GATCGCCCAGATCTCAGCCTAACGGTGCTTCCCAAGCTTCAGGCCAGGGAGAGAGGTGAAGAACAAGTGG; AGCTCTCCACAATTGAGGAACTGATCAAGGATGCCATAGTCAGCACCCAGCCAGCCATGATGGTCAACCT; CAGGGCTTGCTCTGCCCCAGGAGGCCTGGTACCCAGTGAGAAGCCACCCATGATGCCCCAGGCTCAGCCA; GCCATCCCCAGACCTAACCGGTTATTCCTACGGCAGCTTCGGGCATCTCACTTGGGAAATGAGCTGGAAG; GCACCGAGGAACTGTGCTGTGTAGCTGAACTCGACAACCCCATGCAGCAGAAGTGGACCAAGCCCGCGAG; GGCTGGATCCGAGGTGGAGTGGACAGAAGACCTGGCACTGGATCTGGGCCCCCAGAGCCGGGAGCTGACC; CTCAAAGTGCTGAGGAGCAGCAGCTGTGGAGACACCGAACTCCTAGGCCAGGCCACACTGCCTGTGGGCT; CCCCCTCCAGACCACTGTCTCGAAGACAGTTGTGCCCACTCACCCCAGGGCCAGGGAAAGCCCTGGGACC; AGCAGCCACCATGGCAGTGGAGCTTCACTATGAGGAGGGCTCTCCCCGGAACCTGGGTACTCCCACCTCC; TCCACTCCACGCCCCAGCATCACACCTACCAAGAAGATTGAGCTTGACCGGACCATCATGCCCGATGGCA; CCATTGTCACCACAGTCACCACTGTCCAGTCCCGGCCCCGTATAGACGGCAAATTAGACTCCCCCTCCCG; CTCCCCGTCCAAGGTGGAGGTGACCGAGAAGACGACAACTGTGCTGAGTGAGAGCAGTGGCCCCAGCAAT; ACCTCCCATAGCAGCAGCCGGGACAGCCACCTTTCCAACGGCTTGGACCCTGTAGCAGAGACAGCGATTC; GCCAGCTGACAGAGCCCAGTGGGCGGGTGGCCAAGAAGACACCCACCAAGCGCAGCACTCTCATCATCTC; TGGTGTTTCCAAGGTGCCCATTGCTCAGGACGAGTTGGCGCTATCCCTGGGCTATGCGGCATCCCTGGAA; GCCTCAGTGCAGGATGATGCAGGGACCAGCGGAGGCCCCTCTTCACCTCCCTCAGACCCACCAGCCATGT; CTCCAGGACCGCTAGATGCCCTCTCTAGTCCCACAAGTGTCCAGGAAGCAGACGAGACAACCCGTTCGGA; TATTTCTGAGAGGCCATCTGTGGATGATATTGAGTCGGAAACGGGGTCCACTGGTGCCCTGGAGACCCGC; AGCCTCAAGGATCACAAAGTGAGTTTCCTGCGCAGCGGCACTAAGCTCATCTTCCGCCGGAGGCCTAGGC; AGAAGGAAGCTGGCCTGAGCCAATCACACGATGACCTCTCCAACGCAACGGCCACGCCCAGTGTCCGAAA; GAAGGCCGGCAGCTTTTCTCGCCGCCTTATCAAGCGCTTTTCCTTCAAATCCAAACCCAAGGCCAATGGT; AACCCCAGCCCCCAGCTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203922 representing NM_014807; Red=Cloning site Green=Tags(s); ; MDPGWGQRDVGWAALLILFAASLLTVFAWLLQYARGLWLARARGDRGPGPALAGEPAGSLRELGVWRSLL; RLRATRAGAAEEPGVRGLLASLFAFKSFRENWQRAWVRALNEQACRNGSSIQIAFEEVPQLPPRASISHV; TCVDQSEHTMVLRCQLSAEEVRFPVSVTQQSPAAVSMETYHVTLTLPPTQLEVNLEEIPGEGLLISWAFT; DRPDLSLTVLPKLQARERGEEQVELSTIEELIKDAIVSTQPAMMVNLRACSAPGGLVPSEKPPMMPQAQP; AIPRPNRLFLRQLRASHLGNELEGTEELCCVAELDNPMQQKWTKPARAGSEVEWTEDLALDLGPQSRELT; LKVLRSSSCGDTELLGQATLPVGSPSRPLSRRQLCPLTPGPGKALGPAATMAVELHYEEGSPRNLGTPTS; STPRPSITPTKKIELDRTIMPDGTIVTTVTTVQSRPRIDGKLDSPSRSPSKVEVTEKTTTVLSESSGPSN; TSHSSSRDSHLSNGLDPVAETAIRQLTEPSGRVAKKTPTKRSTLIISGVSKVPIAQDELALSLGYAASLE; ASVQDDAGTSGGPSSPPSDPPAMSPGPLDALSSPTSVQEADETTRSDISERPSVDDIESETGSTGALETR; SLKDHKVSFLRSGTKLIFRRRPRQKEAGLSQSHDDLSNATATPSVRKKAGSFSRRLIKRFSFKSKPKANG; NPSPQL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014807
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014807.2
• RefSeq Size: 3370 bp
• RefSeq ORF: 2124 bp
• Locus ID: 9854
• UniProt ID: O14523
• Cytogenetics: 11q23.3
• Protein Families: Transmembrane
• Summary: Lipid-binding protein that transports phosphatidylinositol, the precursor of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), from its site of synthesis in the endoplasmic reticulum to the cell membrane (PubMed:28209843). It thereby maintains the pool of cell membrane phosphoinositides, which are degraded during phospholipase C (PLC) signaling (PubMed:28209843). Plays a key role in the coordination of Ca(2+) and phosphoinositide signaling: localizes to sites of contact between the endoplasmic reticulum and the cell membrane, where it tethers the two bilayers (PubMed:28209843). In response to elevation of cytosolic Ca(2+), it is phosphorylated at its C-terminus and dissociates from the cell membrane, abolishing phosphatidylinositol transport to the cell membrane (PubMed:28209843). Positively regulates insulin secretion in response to glucose: phosphatidylinositol transfer to the cell membrane allows replenishment of PI(4,5)P2 pools and calcium channel opening, priming a new population of insulin granules (PubMed:28209843).[UniProtKB/Swiss-Prot Function]