GANP (MCM3AP) (NM_003906) Human Untagged Clone

SKU SC308999

MCM3AP (untagged)-Human minichromosome maintenance complex component 3 associated protein (MCM3AP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GANP
• Synonyms: GANP; MAP80; PNRIID; SAC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308999 representing NM_003906.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAACCCAACTAATCCTTTCAGTGGGCAGCAGCCTAGTGCTTTTTCGGCGTCTTCTAGTAATGTAGGA; ACACTTCCATCTAAGCCGCCATTTCGATTTGGTCAACCTTCTCTTTTTGGACAAAACAGTACCTTATCT; GGGAAGAGCTCGGGATTTTCACAGGTATCCAGCTTTCCAGCGTCTTCTGGAGTAAGTCATTCCTCTTCA; GTGCAAACATTAGGGTTCACCCAAACCTCAAGTGTTGGACCCTTTTCTGGACTTGAGCACACTTCCACC; TTTGTGGCTACCTCTGGGCCTTCAAGTTCATCTGTGCTGGGAAACACAGGATTTAGTTTTAAATCACCC; ACCAGTGTTGGGGCTTTCCCAAGCACTTCTGCTTTTGGACAAGAAGCTGGAGAAATAGTGAACTCTGGT; TTTGGGAAAACAGAATTCAGCTTTAAACCTCTGGAAAATGCAGTGTTCAAACCAATACTGGGGGCTGAA; TCTGAGCCAGAGAAAACCCAGAGCCAAATTGCTTCTGGGTTTTTTACATTTTCCCACCCAATTAGTAGT; GCACCTGGAGGCCTGGCCCCTTTCTCTTTTCCTCAAGTAACAAGTAGTTCAGCTACCACTTCAAATTTT; ACCTTTTCAAAACCTGTTAGTAGTAATAATTCATTATCTGCCTTTACCCCTGCTTTGTCAAACCAAAAT; GTAGAGGAAGAGAAGAGAGGACCTAAGTCAATATTTGGAAGTTCTAATAATAGCTTCAGTAGCTTCCCT; GTATCATCTGCGGTTTTGGGCGAACCTTTCCAGGCTAGCAAAGCAGGTGTCAGGCAGGGGTGTGAAGAA; GCTGTTTCCCAGGTGGAACCACTTCCCAGCCTAATGAAAGGACTGAAAAGGAAGGAGGACCAGGATCGC; TCCCCAAGGAGACATGGCCACGAGCCAGCAGAAGATTCGGATCCTCTGTCCCGGGGCGATCATCCTCCA; GACAAACGACCTGTCCGCCTGAATCGACCCCGGGGAGGTACTTTATTTGGTCGGACGATACAGGATGTT; TTCAAAAGCAATAAGGAAGTAGGTCGTCTGGGCAACAAGGAGGCCAAAAAGGAAACTGGCTTTGTTGAG; TCTGCAGAAAGTGACCACATGGCTATCCCAGGAGGGAATCAGTCTGTCCTGGCACCTTCCCGGATTCCA; GGTGTGAATAAAGAGGAAGAAACTGAAAGTAGAGAGAAGAAAGAAGATTCTCTAAGAGGAACTCCGGCG; CGTCAGAGTAACAGAAGCGAGAGCACAGACAGTCTTGGGGGCTTGTCTCCCTCTGAAGTCACAGCCATC; CAGTGCAAGAACATCCCTGACTACCTCAACGACAGGACCATTCTGGAGAACCATTTTGGCAAAATTGCT; AAAGTGCAGCGCATCTTTACCAGGCGCAGCAAAAAGCTTGCAGTGGTACATTTCTTTGATCATGCATCT; GCAGCCCTGGCTAGAAAGAAGGGGAAAAGTTTGCATAAAGACATGGCTATCTTTTGGCACAGGAAGAAA; ATAAGCCCCAATAAGAAACCCTTTTCCCTGAAGGAGAAGAAACCAGGTGACGGTGAAGTCAGCCCGAGC; ACAGAGGATGCACCCTTTCAGCACTCTCCTCTTGGCAAGGCCGCAGGGAGGACTGGTGCTAGCAGCCTC; CTGAATAAAAGCTCTCCAGTGAAGAAGCCAAGTCTTCTAAAGGCCCACCAATTCGAGGGAGACTCTTTT; GACTCAGCCTCCGAGGGCTCCGAGGGCCTCGGGCCATGTGTGCTCTCCCTCAGTACCCTGATAGGCACT; GTGGCTGAGACATCCAAGGAGAAGTACCGCCTGCTTGACCAGAGAGACAGGATCATGCGGCAAGCTCGG; GTGAAGAGAACCGATCTGGACAAAGCGAGGACTTTTGTTGGCACCTGCCTGGATATGTGTCCTGAGAAG; GAGAGGTACATGCGGGAGACCCGTAGCCAGCTGAGCGTGTTCGAAGTGGTCCCAGGGACTGACCAGGTG; GACCACGCAGCAGCTGTGAAAGAGTACAGTCGGTCCTCGGCGGATCAGGAGGAGCCCCTGCCCCACGAG; CTGCGGCCCTTGCCAGTGCTCAGCAGGACCATGGACTACCTGGTGACCCAGATCATGGACCAGAAGGAG; GGCAGCCTGCGGGATTGGTATGACTTCGTGTGGAACCGCACGCGTGGCATACGGAAGGATATCACGCAG; CAGCACCTCTGTGACCCCCTGACGGTGTCCCTGATTGAGAAGTGCACCCGGTTTCACATCCACTGTGCC; CACTTCATGTGTGAGGAGCCCATGTCCTCCTTTGATGCCAAGATCAATAATGAGAACATGACCAAGTGC; CTGCAGAGCCTGAAGGAGATGTACCAGGACCTGAGAAACAAGGGTGTCTTCTGTGCCAGCGAAGCGGAG; TTCCAGGGCTACAATGTTCTGCTCAGTCTCAACAAGGGAGACATCCTAAGAGAAGTACAACAGTTCCAT; CCTGCTGTTAGAAACTCATCTGAGGTGAAATTTGCTGTTCAGGCTTTTGCTGCATTGAACAGTAATAAT; TTTGTGAGATTTTTCAAACTGGTCCAGTCAGCTTCTTACCTGAACGCTTGTCTTTTACACTGTTACTTC; AGTCAGATCCGCAAGGATGCTCTCCGGGCGCTCAACTTTGCGTACACGGTGAGCACACAGCGATCTACC; ATCTTTCCCCTGGATGGTGTGGTGCGCATGCTGCTGTTCAGAGACTGTGAAGAGGCCACCGACTTCCTC; ACCTGCCACGGCCTCACCGTTTCCGACGGCTGTGTGGAGCTGAACCGGTCTGCATTCCTGGAACCAGAG; GGATTATCCAAGACCAGGAAGTCGGTGTTTATTACTAGGAAGCTGACGGTGTCAGTCGGGGAAATTGTG; AACGGAGGGCCATTGCCCCCCGTCCCTCGTCATACCCCTGTGTGCAGCTTCAACTCCCAGAACAAGTAC; ATCGGGGAGAGCCTGGCCGCGGAGCTGCCCGTCAGCACCCAGAGACCCGGCTCCGACACAGTGGGCGGA; GGGAGAGGAGAGGAGTGTGGTGTAGAGCCGGATGCACCCCTGTCCAGTCTCCCACAGTCTCTACCAGCC; CCTGCGCCCTCACCAGTGCCTCTGCCTCCTGTCCTGGCACTGACCCCGTCTGTGGCGCCCAGCCTCTTC; CAGCTGTCTGTGCAGCCTGAACCACCGCCTCCAGAGCCCGTGCCCATGTACTCTGACGAGGACCTGGCG; CAGGTGGTGGACGAGCTCATCCAGGAGGCCCTGCAGAGGGACTGTGAGGAAGTTGGCTCTGCGGGTGCT; GCCTACGCAGCTGCCGCCCTGGGTGTTTCTAATGCTGCTATGGAGGATTTGTTAACAGCTGCAACCACG; GGCATTTTGAGGCACATTGCAGCTGAAGAAGTGTCTAAGGAAAGAGAGCGAAGGGAGCAGGAGAGGCAG; CGGGCTGAAGAGGAAAGGTTGAAACAAGAGAGAGAGCTGGTGTTAAGTGAGCTGAGCCAGGGCCTGGCC; GTGGAGCTGATGGAACGCGTGATGATGGAGTTTGTGAGGGAAACCTGCTCCCAGGAGTTGAAGAATGCA; GTAGAGACAGACCAGAGGGTCCGTGTGGCCCGTTGCTGTGAGGATGTCTGTGCCCACTTAGTGGACTTG; TTTCTCGTGGAGGAAATCTTCCAGACTGCAAAGGAGACCCTCCAGGAGCTTCAGTGCTTCTGCAAGTAT; CTACAGCGGTGGAGGGAAGCTGTCACAGCCCGCAAGAAACTGAGGCGCCAAATGCGGGCTTTCCCTGCT; GCGCCCTGCTGCGTGGACGTGAGCGACCGGCTGAGGGCGCTGGCGCCCAGCGCAGAGTGCCCCATTGCT; GAAGAGAACCTGGCCAGGGGCCTCCTGGACCTGGGCCATGCAGGGAGATTGGGCATCTCCTGCACCAGG; TTAAGGCGGCTCAGAAACAAGACAGCTCACCAGATGAAGGTTCAGCACTTCTACCAGCAGCTGCTGAGT; GATGTGGCATGGGCGTCTCTGGACCTGCCATCCCTCGTGGCTGAGCACCTCCCTGGGAGGCAGGAGCAT; GTGTTTTGGAAGCTGGTGCTGGTGTTGCCGGATGTAGAGGAGCAGTCCCCAGAGAGTTGTGGCAGAATT; CTAGCAAATTGGTTAAAAGTCAAGTTCATGGGAGATGAAGGCTCAGTGGATGACACATCCAGCGATGCT; GGTGGGATTCAGACGCTTTCGCTTTTCAACTCACTTAGCAGCAAAGGGGATCAGATGATTTCTGTTAAC; GTGTGTATAAAGGTGGCCCATGGCGCCCTCAGTGATGGTGCCATTGATGCTGTGGAGACACAGAAGGAC; CTCCTGGGAGCCAGTGGGCTCATGCTGCTGCTTCCCCCCAAAATGAAGAGTGAGGACATGGCAGAGGAG; GACGTGTACTGGCTGTCGGCCTTGCTGCAGCTCAAGCAGCTCCTGCAGGCTAAGCCCTTCCAGCCTGCG; CTTCCTCTGGTGGTTCTTGTGCCTAGCCCAGGAGGGGACGCCGTTGAGAAGGAAGTAGAAGATGGTCTG; ATGCTACAGGACTTGGTTTCAGCTAAGCTGATTTCAGATTACACTGTTACCGAGATCCCTGATACCATT; AATGATCTACAAGGTTCAACTAAGGTTTTGCAAGCAGTGCAGTGGCTGGTTTCCCACTGCCCCCATTCC; CTTGACCTCTGCTGCCAGACTCTCATTCAGTACGTCGAAGACGGGATTGGCCATGAGTTTAGTGGCCGC; TTTTTCCATGACAGAAGAGAGAGGCGTCTGGGCGGTCTTGCTTCTCAGGAGCCTGGCGCCATCATTGAG; CTGTTTAACAGTGTGCTGCAGTTCCTGGCTTCTGTGGTGTCCTCTGAACAGCTGTGTGACCTGTCCTGG; CCTGTCACTGAGTTTGCTGAGGCAGGGGGCAGCCGGCTGCTTCCTCACCTGCACTGGAATGCCCCAGAG; CACCTGGCCTGGCTGAAGCAGGCTGTGCTCGGGTTCCAGCTTCCGCAGATGGACCTTCCACCCCTGGGG; GCCCCCTGGCTCCCCGTGTGCTCCATGGTTGTCCAGTACGCCTCCCAGATCCCCAGCTCACGCCAGACA; CAGCCTGTCCTCCAGTCCCAGGTGGAGAACCTGCTCCACAGAACCTACTGTAGGTGGAAGAGCAAGAGT; CCCTCCCCAGTCCATGGGGCAGGCCCCTCGGTCATGGAGATCCCATGGGATGATCTTATCGCCTTGTGT; ATCAACCACAAGCTGAGAGACTGGACGCCCCCCCGGCTTCCTGTTACATCAGAGGCGCTGAGTGAAGAT; GGTCAGATATGTGTGTATTTTTTTAAAAACGATTTGAAAAAATATGATGTTCCTTTGTCGTGGGAACAA; GCCAGGTTGCAGACGCAGAAGGAGCTACAGCTGAGAGAGGGACGTTTGGCAATAAAGCCTTTTCATCCT; TCTGCAAACAATTTTCCCATACCATTGCTTCACATGCACCGTAACTGGAAGAGGAGCACAGAGTGTGCT; CAAGAGGGGAGGATTCCCAGCACAGAGGATCTGATGCGAGGAGCTTCTGCTGAGGAGCTCTTGGCGCAG; TGTTTGTCGAGCAGTCTGCTGCTGGAGAAAGAAGAGAACAAGAGGTTTGAAGATCAGCTTCAGCAATGG; TTGTCTGAAGACTCAGGAGCATTTACGGATTTAACTTCCCTTCCCCTCTATCTTCCTCAGACTCTAGTG; TCTCTTTCTCACACTATTGAACCTGTGATGAAAACATCTGTAACTACTAGCCCACAGAGTGACATGATG; AGGGAGCAACTGCAGCTGTCAGAGGCGACAGGAACGTGTCTAGGCGAACGACTAAAGCACCTGGAAAGG; CTGATCCGGAGTTCAAGGGAAGAGGAAGTTGCCTCTGAGCTCCATCTCTCTGCGCTGCTAGACATGGTG; GACATTTGA; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT; ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA;
• Restriction Sites: SgfI-RsrII
• ACCN: NM_003906
• Insert Size: 5943 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003906.4
• RefSeq Size: 6198 bp
• RefSeq ORF: 5943 bp
• Locus ID: 8888
• UniProt ID: O60318
• Cytogenetics: 21q22.3
• Domains: SAC3_GANP
• MW: 218.4 kDa
• Summary: The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014]