GANP (MCM3AP) (NM_003906) Human Tagged ORF Clone

SKU RG218026

MCM3AP (tGFP-tagged) - Human minichromosome maintenance complex component 3 associated protein (MCM3AP)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GANP
• Synonyms: GANP; MAP80; PNRIID; SAC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218026 representing NM_003906; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAACCCAACTAATCCTTTCAGTGGGCAGCAGCCTAGTGCTTTTTCGGCGTCTTCTAGTAATGTAGGAA; CACTTCCATCTAAGCCGCCATTTCGATTTGGTCAACCTTCTCTTTTTGGACAAAACAGTACCTTATCTGG; GAAGAGCTCGGGATTTTCACAGGTATCCAGCTTTCCAGCGTCTTCTGGAGTAAGTCATTCCTCTTCAGTG; CAAACATTAGGGTTCACCCAAACCTCAAGTGTTGGACCCTTTTCTGGACTTGAGCACACTTCCACCTTTG; TGGCTACCTCTGGGCCTTCAAGTTCATCTGTGCTGGGAAACACAGGATTTAGTTTTAAATCACCCACCAG; TGTTGGGGCTTTCCCAAGCACTTCTGCTTTTGGACAAGAAGCTGGAGAAATAGTGAACTCTGGTTTTGGG; AAAACAGAATTCAGCTTTAAACCTCTGGAAAATGCAGTGTTCAAACCAATACTGGGGGCTGAATCTGAGC; CAGAGAAAACCCAGAGCCAAATTGCTTCTGGGTTTTTTACATTTTCCCACCCAATTAGTAGTGCACCTGG; AGGCCTGGCCCCTTTCTCTTTTCCTCAAGTAACAAGTAGTTCAGCTACCACTTCAAATTTTACCTTTTCA; AAACCTGTTAGTAGTAATAATTCATTATCTGCCTTTACCCCTGCTTTGTCAAACCAAAATGTAGAGGAAG; AGAAGAGAGGACCTAAGTCAATATTTGGAAGTTCTAATAATAGCTTCAGTAGCTTCCCTGTATCATCTGC; GGTTTTGGGCGAACCTTTCCAGGCTAGCAAAGCAGGTGTCAGGCAGGGGTGTGAAGAAGCTGTTTCCCAG; GTGGAACCACTTCCCAGCCTAATGAAAGGACTGAAAAGGAAGGAGGACCAGGATCGCTCCCCAAGGAGAC; ATGGCCACGAGCCAGCAGAAGATTCGGATCCTCTGTCCCGGGGCGATCATCCTCCAGACAAACGACCTGT; CCGCCTGAATCGACCCCGGGGAGGTACTTTATTTGGTCGGACGATACAGGATGTTTTCAAAAGCAATAAG; GAAGTAGGTCGTCTGGGCAACAAGGAGGCCAAAAAGGAAACTGGCTTTGTTGAGTCTGCAGAAAGTGACC; ACATGGCTATCCCAGGAGGGAATCAGTCTGTCCTGGCACCTTCCCGGATTCCAGGTGTGAATAAAGAGGA; AGAAACTGAAAGTAGAGAGAAGAAAGAAGATTCTCTAAGAGGAACTCCGGCGCGTCAGAGTAACAGAAGC; GAGAGCACAGACAGTCTTGGGGGCTTGTCTCCCTCTGAAGTCACAGCCATCCAGTGCAAGAACATCCCTG; ACTACCTCAACGACAGGACCATTCTGGAGAACCATTTTGGCAAAATTGCTAAAGTGCAGCGCATCTTTAC; CAGGCGCAGCAAAAAGCTTGCAGTGGTACATTTCTTTGATCATGCATCTGCAGCCCTGGCTAGAAAGAAG; GGGAAAAGTTTGCATAAAGACATGGCTATCTTTTGGCACAGGAAGAAAATAAGCCCCAATAAGAAACCCT; TTTCCCTGAAGGAGAAGAAACCAGGTGACGGTGAAGTCAGCCCGAGCACAGAGGATGCACCCTTTCAGCA; CTCTCCTCTTGGCAAGGCCGCAGGGAGGACTGGTGCTAGCAGCCTCCTGAATAAAAGCTCTCCAGTGAAG; AAGCCAAGTCTTCTAAAGGCCCACCAATTCGAGGGAGACTCTTTTGACTCAGCCTCCGAGGGCTCCGAGG; GCCTCGGGCCATGTGTGCTCTCCCTCAGTACCCTGATAGGCACTGTGGCTGAGACATCCAAGGAGAAGTA; CCGCCTGCTTGACCAGAGAGACAGGATCATGCGGCAAGCTCGGGTGAAGAGAACCGATCTGGACAAAGCG; AGGACTTTTGTTGGCACCTGCCTGGATATGTGTCCTGAGAAGGAGAGGTACATGCGGGAGACCCGTAGCC; AGCTGAGCGTGTTCGAAGTGGTCCCAGGGACTGACCAGGTGGACCACGCAGCAGCTGTGAAAGAGTACAG; TCGGTCCTCGGCGGATCAGGAGGAGCCCCTGCCCCACGAGCTGCGGCCCTTGCCAGTGCTCAGCAGGACC; ATGGACTACCTGGTGACCCAGATCATGGACCAGAAGGAGGGCAGCCTGCGGGATTGGTATGACTTCGTGT; GGAACCGCACGCGTGGCATACGGAAGGATATCACGCAGCAGCACCTCTGTGACCCCCTGACGGTGTCCCT; GATTGAGAAGTGCACCCGGTTTCACATCCACTGTGCCCACTTCATGTGTGAGGAGCCCATGTCCTCCTTT; GATGCCAAGATCAATAATGAGAACATGACCAAGTGCCTGCAGAGCCTGAAGGAGATGTACCAGGACCTGA; GAAACAAGGGTGTCTTCTGTGCCAGCGAAGCGGAGTTCCAGGGCTACAATGTTCTGCTCAGTCTCAACAA; GGGAGACATCCTAAGAGAAGTACAACAGTTCCATCCTGCTGTTAGAAACTCATCTGAGGTGAAATTTGCT; GTTCAGGCTTTTGCTGCATTGAACAGTAATAATTTTGTGAGATTTTTCAAACTGGTCCAGTCAGCTTCTT; ACCTGAACGCTTGTCTTTTACACTGTTACTTCAGTCAGATCCGCAAGGATGCTCTCCGGGCGCTCAACTT; TGCGTACACGGTGAGCACACAGCGATCTACCATCTTTCCCCTGGATGGTGTGGTGCGCATGCTGCTGTTC; AGAGACTGTGAAGAGGCCACCGACTTCCTCACCTGCCACGGCCTCACCGTTTCCGACGGCTGTGTGGAGC; TGAACCGGTCTGCATTCCTGGAACCAGAGGGATTATCCAAGACCAGGAAGTCGGTGTTTATTACTAGGAA; GCTGACGGTGTCAGTCGGGGAAATTGTGAACGGAGGGCCATTGCCCCCCGTCCCTCGTCATACCCCTGTG; TGCAGCTTCAACTCCCAGAACAAGTACATCGGGGAGAGCCTGGCCGCGGAGCTGCCCGTCAGCACCCAGA; GACCCGGCTCCGACACAGTGGGCGGAGGGAGAGGAGAGGAGTGTGGTGTAGAGCCGGATGCACCCCTGTC; CAGTCTCCCACAGTCTCTACCAGCCCCTGCGCCCTCACCAGTGCCTCTGCCTCCTGTCCTGGCACTGACC; CCGTCTGTGGCGCCCAGCCTCTTCCAGCTGTCTGTGCAGCCTGAACCACCGCCTCCAGAGCCCGTGCCCA; TGTACTCTGACGAGGACCTGGCGCAGGTGGTGGACGAGCTCATCCAGGAGGCCCTGCAGAGGGACTGTGA; GGAAGTTGGCTCTGCGGGTGCTGCCTACGCAGCTGCCGCCCTGGGTGTTTCTAATGCTGCTATGGAGGAT; TTGTTAACAGCTGCAACCACGGGCATTTTGAGGCACATTGCAGCTGAAGAAGTGTCTAAGGAAAGAGAGC; GAAGGGAGCAGGAGAGGCAGCGGGCTGAAGAGGAAAGGTTGAAACAAGAGAGAGAGCTGGTGTTAAGTGA; GCTGAGCCAGGGCCTGGCCGTGGAGCTGATGGAACGCGTGATGATGGAGTTTGTGAGGGAAACCTGCTCC; CAGGAGTTGAAGAATGCAGTAGAGACAGACCAGAGGGTCCGTGTGGCCCGTTGCTGTGAGGATGTCTGTG; CCCACTTAGTGGACTTGTTTCTCGTGGAGGAAATCTTCCAGACTGCAAAGGAGACCCTCCAGGAGCTTCA; GTGCTTCTGCAAGTATCTACAGCGGTGGAGGGAAGCTGTCACAGCCCGCAAGAAACTGAGGCGCCAAATG; CGGGCTTTCCCTGCTGCGCCCTGCTGCGTGGACGTGAGCGACCGGCTGAGGGCGCTGGCGCCCAGCGCAG; AGTGCCCCATTGCTGAAGAGAACCTGGCCAGGGGCCTCCTGGACCTGGGCCATGCAGGGAGATTGGGCAT; CTCCTGCACCAGGTTAAGGCGGCTCAGAAACAAGACAGCTCACCAGATGAAGGTTCAGCACTTCTACCAG; CAGCTGCTGAGTGATGTGGCATGGGCGTCTCTGGACCTGCCATCCCTCGTGGCTGAGCACCTCCCTGGGA; GGCAGGAGCATGTGTTTTGGAAGCTGGTGCTGGTGTTGCCGGATGTAGAGGAGCAGTCCCCAGAGAGTTG; TGGCAGAATTCTAGCAAATTGGTTAAAAGTCAAGTTCATGGGAGATGAAGGCTCAGTGGATGACACATCC; AGCGATGCTGGTGGGATTCAGACGCTTTCGCTTTTCAACTCACTTAGCAGCAAAGGGGATCAGATGATTT; CTGTTAACGTGTGTATAAAGGTGGCCCATGGCGCCCTCAGTGATGGTGCCATTGATGCTGTGGAGACACA; GAAGGACCTCCTGGGAGCCAGTGGGCTCATGCTGCTGCTTCCCCCCAAAATGAAGAGTGAGGACATGGCA; GAGGAGGACGTGTACTGGCTGTCGGCCTTGCTGCAGCTCAAGCAGCTCCTGCAGGCTAAGCCCTTCCAGC; CTGCGCTTCCTCTGGTGGTTCTTGTGCCTAGCCCAGGAGGGGACGCCGTTGAGAAGGAAGTAGAAGATGG; TCTGATGCTACAGGACTTGGTTTCAGCTAAGCTGATTTCAGATTACACTGTTACCGAGATCCCTGATACC; ATTAATGATCTACAAGGTTCAACTAAGGTTTTGCAAGCAGTGCAGTGGCTGGTTTCCCACTGCCCCCATT; CCCTTGACCTCTGCTGCCAGACTCTCATTCAGTACGTCGAAGACGGGATTGGCCATGAGTTTAGTGGCCG; CTTTTTCCATGACAGAAGAGAGAGGCGTCTGGGCGGTCTTGCTTCTCAGGAGCCTGGCGCCATCATTGAG; CTGTTTAACAGTGTGCTGCAGTTCCTGGCTTCTGTGGTGTCCTCTGAACAGCTGTGTGACCTGTCCTGGC; CTGTCACTGAGTTTGCTGAGGCAGGGGGCAGCCGGCTGCTTCCTCACCTGCACTGGAATGCCCCAGAGCA; CCTGGCCTGGCTGAAGCAGGCTGTGCTCGGGTTCCAGCTTCCGCAGATGGACCTTCCACCCCTGGGGGCC; CCCTGGCTCCCCGTGTGCTCCATGGTTGTCCAGTACGCCTCCCAGATCCCCAGCTCACGCCAGACACAGC; CTGTCCTCCAGTCCCAGGTGGAGAACCTGCTCCACAGAACCTACTGTAGGTGGAAGAGCAAGAGTCCCTC; CCCAGTCCATGGGGCAGGCCCCTCGGTCATGGAGATCCCATGGGATGATCTTATCGCCTTGTGTATCAAC; CACAAGCTGAGAGACTGGACGCCCCCCCGGCTTCCTGTTACATCAGAGGCGCTGAGTGAAGATGGTCAGA; TATGTGTGTATTTTTTTAAAAACGATTTGAAAAAATATGATGTTCCTTTGTCGTGGGAACAAGCCAGGTT; GCAGACGCAGAAGGAGCTACAGCTGAGAGAGGGACGTTTGGCAATAAAGCCTTTTCATCCTTCTGCAAAC; AATTTTCCCATACCATTGCTTCACATGCACCGTAACTGGAAGAGGAGCACAGAGTGTGCTCAAGAGGGGA; GGATTCCCAGCACAGAGGATCTGATGCGAGGAGCTTCTGCTGAGGAGCTCTTGGCGCAGTGTTTGTCGAG; CAGTCTGCTGCTGGAGAAAGAAGAGAACAAGAGGTTTGAAGATCAGCTTCAGCAATGGTTGTCTGAAGAC; TCAGGAGCATTTACGGATTTAACTTCCCTTCCCCTCTATCTTCCTCAGACTCTAGTGTCTCTTTCTCACA; CTATTGAACCTGTGATGAAAACATCTGTAACTACTAGCCCACAGAGTGACATGATGAGGGAGCAACTGCA; GCTGTCAGAGGCGACAGGAACGTGTCTAGGCGAACGACTAAAGCACCTGGAAAGGCTGATCCGGAGTTCA; AGGGAAGAGGAAGTTGCCTCTGAGCTCCATCTCTCTGCGCTGCTAGACATGGTGGACATT; ; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218026 representing NM_003906; Red=Cloning site Green=Tags(s); ; MNPTNPFSGQQPSAFSASSSNVGTLPSKPPFRFGQPSLFGQNSTLSGKSSGFSQVSSFPASSGVSHSSSV; QTLGFTQTSSVGPFSGLEHTSTFVATSGPSSSSVLGNTGFSFKSPTSVGAFPSTSAFGQEAGEIVNSGFG; KTEFSFKPLENAVFKPILGAESEPEKTQSQIASGFFTFSHPISSAPGGLAPFSFPQVTSSSATTSNFTFS; KPVSSNNSLSAFTPALSNQNVEEEKRGPKSIFGSSNNSFSSFPVSSAVLGEPFQASKAGVRQGCEEAVSQ; VEPLPSLMKGLKRKEDQDRSPRRHGHEPAEDSDPLSRGDHPPDKRPVRLNRPRGGTLFGRTIQDVFKSNK; EVGRLGNKEAKKETGFVESAESDHMAIPGGNQSVLAPSRIPGVNKEEETESREKKEDSLRGTPARQSNRS; ESTDSLGGLSPSEVTAIQCKNIPDYLNDRTILENHFGKIAKVQRIFTRRSKKLAVVHFFDHASAALARKK; GKSLHKDMAIFWHRKKISPNKKPFSLKEKKPGDGEVSPSTEDAPFQHSPLGKAAGRTGASSLLNKSSPVK; KPSLLKAHQFEGDSFDSASEGSEGLGPCVLSLSTLIGTVAETSKEKYRLLDQRDRIMRQARVKRTDLDKA; RTFVGTCLDMCPEKERYMRETRSQLSVFEVVPGTDQVDHAAAVKEYSRSSADQEEPLPHELRPLPVLSRT; MDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTVSLIEKCTRFHIHCAHFMCEEPMSSF; DAKINNENMTKCLQSLKEMYQDLRNKGVFCASEAEFQGYNVLLSLNKGDILREVQQFHPAVRNSSEVKFA; VQAFAALNSNNFVRFFKLVQSASYLNACLLHCYFSQIRKDALRALNFAYTVSTQRSTIFPLDGVVRMLLF; RDCEEATDFLTCHGLTVSDGCVELNRSAFLEPEGLSKTRKSVFITRKLTVSVGEIVNGGPLPPVPRHTPV; CSFNSQNKYIGESLAAELPVSTQRPGSDTVGGGRGEECGVEPDAPLSSLPQSLPAPAPSPVPLPPVLALT; PSVAPSLFQLSVQPEPPPPEPVPMYSDEDLAQVVDELIQEALQRDCEEVGSAGAAYAAAALGVSNAAMED; LLTAATTGILRHIAAEEVSKERERREQERQRAEEERLKQERELVLSELSQGLAVELMERVMMEFVRETCS; QELKNAVETDQRVRVARCCEDVCAHLVDLFLVEEIFQTAKETLQELQCFCKYLQRWREAVTARKKLRRQM; RAFPAAPCCVDVSDRLRALAPSAECPIAEENLARGLLDLGHAGRLGISCTRLRRLRNKTAHQMKVQHFYQ; QLLSDVAWASLDLPSLVAEHLPGRQEHVFWKLVLVLPDVEEQSPESCGRILANWLKVKFMGDEGSVDDTS; SDAGGIQTLSLFNSLSSKGDQMISVNVCIKVAHGALSDGAIDAVETQKDLLGASGLMLLLPPKMKSEDMA; EEDVYWLSALLQLKQLLQAKPFQPALPLVVLVPSPGGDAVEKEVEDGLMLQDLVSAKLISDYTVTEIPDT; INDLQGSTKVLQAVQWLVSHCPHSLDLCCQTLIQYVEDGIGHEFSGRFFHDRRERRLGGLASQEPGAIIE; LFNSVLQFLASVVSSEQLCDLSWPVTEFAEAGGSRLLPHLHWNAPEHLAWLKQAVLGFQLPQMDLPPLGA; PWLPVCSMVVQYASQIPSSRQTQPVLQSQVENLLHRTYCRWKSKSPSPVHGAGPSVMEIPWDDLIALCIN; HKLRDWTPPRLPVTSEALSEDGQICVYFFKNDLKKYDVPLSWEQARLQTQKELQLREGRLAIKPFHPSAN; NFPIPLLHMHRNWKRSTECAQEGRIPSTEDLMRGASAEELLAQCLSSSLLLEKEENKRFEDQLQQWLSED; SGAFTDLTSLPLYLPQTLVSLSHTIEPVMKTSVTTSPQSDMMREQLQLSEATGTCLGERLKHLERLIRSS; REEEVASELHLSALLDMVDI; ; SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003906
• ORF Size: 5940 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003906.5
• RefSeq Size: 6113 bp
• RefSeq ORF: 5943 bp
• Locus ID: 8888
• UniProt ID: O60318
• Cytogenetics: 21q22.3
• Domains: SAC3_GANP
• Summary: The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014]