SIT (SIT1) (NM_014450) Human Untagged Clone

SKU
SC304112
SIT1 (untagged)-Human signaling threshold regulating transmembrane adaptor 1 (SIT1)
$330.00
3 Weeks*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SIT
Synonyms SIT; SIT-R
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC304112 representing NM_014450.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAACCAGGCTGACCCTCGGCTCAGAGCAGTGTGCTTGTGGACTCTCACATCTGCAGCCATGAGCAGA
GGCGACAACTGCACGGATCTACTCGCACTGGGAATCCCCTCCATAACCCAGGCCTGGGGACTGTGGGTC
CTCTTAGGGGCTGTGACGCTGCTATTTCTCATCTCGCTGGCTGCACACTTGTCCCAGTGGACCAGGGGC
CGGAGCAGGAGCCATCCGGGGCAGGGACGCTCTGGAGAGTCTGTGGAAGAGGTCCCGCTGTATGGGAAC
CTGCATTATCTACAGACAGGACGGCTGTCTCAAGACCCAGAGCCAGACCAGCAGGATCCAACTCTTGGA
GGCCCTGCCAGGGCTGCAGAGGAGGTGATGTGCTATACCAGCCTGCAGCTGCGGCCTCCTCAGGGTCGG
ATCCCCGGTCCTGGAACCCCCGTCAAGTACTCGGAGGTGGTGCTGGACTCTGAGCCAAAGTCCCAGGCC
TCGGGCCCCGAGCCGGAGCTCTATGCCTCAGTATGTGCCCAGACCCGCAGGGCCCGGGCCTCCTTCCCG
GATCAGGCCTATGCCAACAGCCAGCCTGCAGCCAGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI
ACCN NM_014450
Insert Size 591 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014450.2
RefSeq Size 1238 bp
RefSeq ORF 591 bp
Locus ID 27240
UniProt ID Q9Y3P8
Cytogenetics 9p13.3
Protein Families Transmembrane
MW 21.1 kDa
Summary Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.