SIT (SIT1) (NM_014450) Human Tagged Lenti ORF Clone

SKU
RC211968L2
Lenti ORF clone of Human signaling threshold regulating transmembrane adaptor 1 (SIT1), mGFP tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$600.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SIT
Synonyms SIT; SIT-R
Vector pLenti-C-mGFP
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC211968).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_014450
ORF Size 588 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014450.2
RefSeq Size 1238 bp
RefSeq ORF 591 bp
Locus ID 27240
UniProt ID Q9Y3P8
Cytogenetics 9p13.3
Protein Families Transmembrane
MW 21.1 kDa
Summary Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC211968 SIT1 (Myc-DDK-tagged)-Human signaling threshold regulating transmembrane adaptor 1 (SIT1) 10 ug
$300.00
RC211968L1 Lenti ORF clone of Human signaling threshold regulating transmembrane adaptor 1 (SIT1), Myc-DDK-tagged 10 ug
$600.00
RC211968L3 Lenti ORF clone of Human signaling threshold regulating transmembrane adaptor 1 (SIT1), Myc-DDK-tagged 10 ug
$600.00
RC211968L4 Lenti ORF clone of Human signaling threshold regulating transmembrane adaptor 1 (SIT1), mGFP tagged 10 ug
$600.00
RG211968 SIT1 (tGFP-tagged) - Human signaling threshold regulating transmembrane adaptor 1 (SIT1) 10 ug
$489.00 MSRP $500.00 MSRP $500.00
SC304112 SIT1 (untagged)-Human signaling threshold regulating transmembrane adaptor 1 (SIT1) 10 ug
$330.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.