PPAR gamma (PPARG) (NM_138712) Human Untagged Clone

CAT#: SC124177

PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPAR gamma
• Synonyms: CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138712 edited
  GGCGCCCGCGCCCGCCCCCGCGCCGGGCCCGGCTCGGCCCGACCCGGCTCCGCCGCGGGC
  AGGCGGGGCCCAGCGCACTCGGAGCCCGAGCCCGAGCCGCAGCTGCCGCCTGGGGCGCTT
  GGGTCGGCCTCGAGGACACCGGAGAGGGGCGCCACGCCGCCGTGGCCGCAGATTTGAAAG
  AAGCCAACACTAAACCACAAATATACAACAAGGCCATTTTCTCAAACGAGAGTCAGCCTT
  TAACGAAATGACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTTGGGATCAG
  CTCCGTGGATCTCTCCGTAATGGAAGACCACTCCCACTCCTTTGATATCAAGCCCTTCAC
  TACTGTTGACTTCTCCAGCATTTCTACTCCACATTACGAAGACATTCCATTCACAAGAAC
  AGATCCAGTGGTTGCAGATTACAAGTATGACCTGAAACTTCAAGAGTACCAAAGTGCAAT
  CAAAGTGGAGCCTGCATCTCCACCTTATTATTCTGAGAAGACTCAGCTCTACAATAAGCC
  TCATGAAGAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGTGTCTGTGGAGATAAAGC
  TTCTGGATTTCACTATGGAGTTCATGCTTGTGAAGGATGCAAGGGTTTCTTCCGGAGAAC
  AATCAGATTGAAGCTTATCTATGACAGATGTGATCTTAACTGTCGGATCCACAAAAAAAG
  TAGAAATAAATGTCAGTACTGTCGGTTTCAGAAATGCCTTGCAGTGGGGATGTCTCATAA
  TGCCATCAGGTTTGGGCGGATGCCACAGGCCGAGAAGGAGAAGCTGTTGGCGGAGATCTC
  CAGTGATATCGACCAGCTGAATCCAGAGTCCGCTGACCTCCGGGCCCTGGCAAAACATTT
  GTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCAAAGGCGAGGGCGATCTTGAC
  AGGAAAGACAACAGACAAATCACCATTCGTTATCTATGACATGAATTCCTTAATGATGGG
  AGAAGATAAAATCAAGTTCAAACACATCACCCCCCTGCAGGAGCAGAGCAAAGAGGTGGC
  CATCCGCATCTTTCAGGGCTGCCAGTTTCGCTCCGTGGAGGCTGTGCAGGAGATCACAGA
  GTATGCCAAAAGCATTCCTGGTTTTGTAAATCTTGACTTGAACGACCAAGTAACTCTCCT
  CAAATATGGAGTCCACGAGATCATTTACACAATGCTGGCCTCCTTGATGAATAAAGATGG
  GGTTCTCATATCCGAGGGCCAAGGCTTCATGACAAGGGAGTTTCTAAAGAGCCTGCGAAA
  GCCTTTTGGTGACTTTATGGAGCCCAAGTTTGAGTTTGCTGTGAAGTTCAATGCACTGGA
  ATTAGATGACAGCGACTTGGCAATATTTATTGCTGTCATTATTCTCAGTGGAGACCGCCC
  AGGTTTGCTGAATGTGAAGCCCATTGAAGACATTCAAGACAACCTGCTACAAGCCCTGGA
  GCTCCAGCTGAAGCTGAACCACCCTGAGTCCTCACAGCTGTTTGCCAAGCTGCTCCAGAA
  AATGACAGACCTCAGACAGATTGTCACGGAACACGTGCAGCTACTGCAGGTGATCAAGAA
  GACGGAGACAGACATGAGTCTTCACCCGCTCCTGCAGGAGATCTACAAGGACTTGTACTA
  GCAGAGAGTCCTGAGCCACTGCCAACATTTCCCTTCTTCCAGTTGCACTATTCTGAGGGA
  AAATCTGACACCTAAGAAATTTACTGTGAAAAAGCATTTTAAAAAGAAAAGGTTTTAGAA
  TATGATCTATTTTATGCATATTGTTTATAAAGACACATTTACAATTTACTTTTAATAT
  TAAAAATTACCATATTATGAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138712 unedited
  CCAGCATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACCANAGCCGGCG
  CCCGCGCCCGCCCCCGCGCCGGGCCCGGCTCGGCCCGACCCGGCTCCGCCGCGGGCAGGC
  GGGGCCCAGCGCACTCGGAGCCCGAGCCCGAGCCGCAGCTGCCGCCTGGGGCGCTTGGGT
  CGGCCTCGAGGACACCGGAGAGGGGCGCCACGCCGCCGTGGCCGCAGATTTGAAAGAAGC
  CAACACTAAACCACAAATATACAACAAGGCCATTTTCTCAAACGAGAGTCAGCCTTTAAC
  GAAATGACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTTGGGATCAGCTCC
  GTGGATCTCTCCGTAATGGAAGACCACTCCCACTCCTTTGATATCAAGCCCTTCACTACT
  GTTGACTTCTCCAGCATTTCTACTCCACATTACGAAGACATTCCATTCACAAGAACAGAT
  CCAGTGGTTGCAGATTACAAGTATGACCTGAAACTTCAAGAGTACCAAAGTGCAATCAAA
  GTGGAGCCTGCATCTCCACCTTATTATTCTGAGAAGACTCAGCTCTACAATAAGCCTCAT
  GAAGAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGTGTCTGTGGAGATAAAGCTTCT
  GGATTTCACTATGGAGTTCATGCTTGTGAAGGGATGCAGGGTTTCTTCCGGAGAAACATC
  AGATTGAAGCTTATCTATGACAGATGTGATCTTAACTGTCGGATCCACAAAAAAAGTAGA
  AATAAATGTCAGTACTGTCGGTTTCAGAAATGCCTTGCAGTGGGGATGTCTCATAATGCC
  ATCAGGTTTGGGGCGGATGCCACAGGCCGAGNAAGAGAAAGCTGTGGCCGGAAATCTCCA
  GTGATATCGACCAGCTGAATCCAGAGTCGCTGACCTCCGGCCCTGGCAAACTTTGTATGA
  CTCATCATAAAGTCTTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138712 unedited
  GCCGCGCCATTTGGGTGATGGCAACTTCCCAGTTTCGGNAAAGCACTGGGGCAGGGTCAC
  AGGGATGCCACCCGGGTTCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGT
  CGAGTTTTTTTTTTTTTTTTTTTTCATAATATGGTAATTTTTAATATTAAAAGTAAATTG
  TAAATGTGTCTTTATAAACAATATGCATAAAATAGATCATATTCTAAAACCTTTTCTTTT
  TAAAATGCTTTTTCACAGTAAATTTCTTAGGTGTCAGATTTTCCCTCAGAATAGTGCAAC
  TGGAAAAAGGGAAATGTTGGCAGTGGCTCAGGACTCTCTGCTAGTACAAGTCCTTGTAGA
  TCTCCTGCAGGAGCGGGTGAAGACTCATGTCTGTCTCCGTTTTCTTGATCACCTGCAGTA
  GCTGCACGTGTTCCGTGACAATCTGTCTGAGGTCTGTCATTTTCTGGAGCAGCTTGGCAA
  ACAGCTGTGAGGACTCAGGGTGGTTCAGCTTCAGCTGGAGCTCCAGGGCTTGTAGCAGGT
  TGTCTTGAATGTCTTCAATGGGCTTCACATTCAGAAAACCTGGGCGGTCTCCACTGAGAA
  TAATGACAGCAATAAATATTGCCAAGTCGCTGTCATCTAATTCCAGTGCATTGAACTTCA
  CAGCAAACTCAAACTTGGGCTCAATAAAGTGACCAAAAGGCTTTCGCAGGCTCTTTAGAA
  ACTCCCTTGTCATGAAGTCTTGGCCCTCCGAATATGAAACCCTATCTTTTTTCTTAAGGA
  GGCCCGCTTGGGGTAATGAATCTCGGGACTCCATATTTTAAGAGAATACCTCGTCCGTTA
  AGTCAATATTACAAACCAGGAATGCTTTTGGCATCTGTGGTGGACCCCAGCAAAACCTCG
  CAGAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138712
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138712.2, NP_619726.1
• RefSeq Size: 1883 bp
• RefSeq ORF: 1428 bp
• Locus ID: 5468
• UniProt ID: P37231
• Cytogenetics: 3p25.2
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer
• Gene Summary: This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR and 5' coding region compared to variant 2. Variants 1, 3, 4, 6, and 7 all encode the same isoform (1), which has a shorter, distinct N-terminus compared to isoform 2.