TSSC3 (PHLDA2) (NM_003311) Human Untagged Clone

SKU SC120954

PHLDA2 (untagged)-Human pleckstrin homology-like domain, family A, member 2 (PHLDA2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TSSC3
• Synonyms: BRW1C; BWR1C; HLDA2; IPL; TSSC3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003311 edited; CCGTCACCGCCCGCATTGCCGCTCCCAGTCCCGCGCTCGGCACGACATGAAATCCCCCGA; CGAGGTGCTACGCGAGGGCGAGTTGGAGAAGCGCAGCGACAGCCTCTTCCAGCTATGGAA; GAAGAAGCGCGGGGTGCTCACCTCCGACCGCCTGAGCCTGTTCCCCGCCAGCCCCCGCGC; GCGCCCCAAGGAGCTGCGCTTCCACTCCATCCTCAAGGTGGACTGCGTGGAGCGCACGGG; CAAGTACGTGTACTTCACCATCGTCACCACCGACCACAAGGAGATCGACTTCCGCTGCGC; GGGCGAGAGCTGCTGGAACGCGGCCATCGCGCTGGCGCTCATCGATTTCCAGAACCGCCG; CGCCCTGCAGGACTTTCGCAGCCGCCAGGAACGCACCGCACCCGCCGCACCCGCCGAGGA; CGCCGTGGCTGCCGCGGCCGCCGCACCCTCCGAGCCCTCGGAGCCCTCCAGGCCATCCCC; GCAGCCCAAACCCCGCACGCCATGAGCCCGCCGCGGGCCATACGCTGGACGAGTCGGACC; GAGGCTAGGACGTGGCCGGCGCTCTCCAGCCCTGCAGCAGAAGAACTTCCCGTGCGCGCG; GATCCTCGCTCCGTTGCACGGGCGCCTTAAGTTATTGGACTATCTAATATCTATGTATTT; ATTTCGCTGGTTCTTTGTAGTCACATATTTTATAGTCTTAATATCTTGTTTTTGCATCAC; TGTGCCCATTGCAAATAAATCACTTGGCCAGTTTGCTTTTCTACCAAAAAAAAAAAAAAA; AAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003311 unedited; AAAACCCTTCGGGATTTGTNATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGG; CCGTCACCGCCCGCATTGCCGCTCCCAGTCCCGCGCTCGGCACGACATGAAATCCCCCGA; CGAGGTGCTACGCGAGGGCGAGTTGGAGAAGCGCAGCGACAGCCTCTTCCAGCTATGGAA; GAAGAAGCGCGGGGTGCTCACCTCCGACCGCCTGAGCCTGTTCCCCGCCAGCCCCCGCGC; GCGCCCCAAGGAGCTGCGCTTCCACTCCATCCTCAAGGTGGACTGCGTGGAGCGCACGGG; CAAGTACGTGTACTTCACCATCGTCACCACCGACCACAAGGAGATCGACTTCCGCTGCGC; GGGCGAGAGCTGCTGGAACGCGGCCATCGCGCTGGCGCTCATCGATTTCCAGAACCGCCG; CGCCCTGCAGGACTTTCGCAGCCGCCAGGAACGCACCGCACCCGCCGCACCCGCCGAGGA; CGCCGTGGCTGCCGCGGCCGCCGCACCCTCCGAGCCCTCGGAGCCCTCCAGGCCATCCCC; GCAGCCCAAACCCCGCACGCCATGAGCCCGCCGCGGGCCATACGCTGGACGAGTCGGACC; GAGGCTAGGACGTGGCCGGCGCTCTCCAGCCCTGCAGCAGAAGAACTTCCCGTGCGCGCG; GATCCTCGCTCCGTTGCACGGGCGCCTTAAGTTATTGGACTATCTAATATCTATGTATTT; ATTTCGCTGGTTCTTTGTAGTCACATATTTTATAGTCTTAATATCTTGTTTTTGCATCAC; TGTGCCCATTGCAAATAAATCACTTGGCCAGTTTGCTTNTCTACCAAAAAAAAAANAAAA; AAACTCGACTCA;
• Restriction Sites: Please inquire
• ACCN: NM_003311
• Insert Size: 784 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003311.3, NP_003302.1
• RefSeq Size: 937 bp
• RefSeq ORF: 459 bp
• Locus ID: 7262
• UniProt ID: Q53GA4
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome
• Summary: This gene is located in a cluster of imprinted genes on chromosome 11p15.5, which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene has been shown to be imprinted, with preferential expression from the maternal allele in placenta and liver. [provided by RefSeq, Oct 2010]