P Glycoprotein (ABCB1) (NM_000927) Human Untagged Clone

CAT#: SC119584

ABCB1 (untagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P Glycoprotein
• Synonyms: ABC20; CD243; CLCS; GP170; MDR1; p-170; P-GP; PGY1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000927 edited
  CAAGCTCAAAGAAGCAGAGGCCGCTGTTCGTTTCCTTTAGGTCTTTCCACTAAAGTCGGA
  GTATCTTCTTCCAAAATTTCACGTCTTGGTGGCCGTTCCAAGGAGCGCGAGGTCGGGATG
  GATCTTGAAGGGGACCGCAATGGAGGAGCAAAGAAGAAGAACTTTTTTAAACTGAACAAT
  AAAAGTGAAAAAGATAAGAAGGAAAAGAAACCAACTGTCAGTGTATTTTCAATGTTTCGC
  TATTCAAATTGGCTTGACAAGTTGTATATGGTGGTGGGAACTTTGGCTGCCATCATCCAT
  GGGGCTGGACTTCCTCTCATGATGCTGGTGTTTGGAGAAATGACAGATATCTTTGCAAAT
  GCAGGAAATTTAGAAGATCTGATGTCAAACATCACTAATAGAAGTGATATCAATGATACA
  GGGTTCTTCATGAATCTGGAGGAAGACATGACCAGGTATGCCTATTATTACAGTGGAATT
  GGTGCTGGGGTGCTGGTTGCTGCTTACATTCAGGTTTCATTTTGGTGCCTGGCAGCTGGA
  AGACAAATACACAAAATTAGAAAACAGTTTTTTCATGCTATAATGCGACAGGAGATAGGC
  TGGTTTGATGTGCACGATGTTGGGGAGCTTAACACCCGACTTACAGATGATGTCTCCAAG
  ATTAATGAAGGAATTGGTGACAAAATTGGAATGTTCTTTCAGTCAATGGCAACATTTTTC
  ACTGGGTTTATAGTAGGATTTACACGTGGTTGGAAGCTAACCCTTGTGATTTTGGCCATC
  AGTCCTGTTCTTGGACTGTCAGCTGCTGTCTGGGCAAAGATACTATCTTCATTTACTGAT
  AAAGAACTCTTAGCGTATGCAAAAGCTGGAGCAGTAGCTGAAGAGGTCTTGGCAGCAATT
  AGAACTGTGATTGCATTTGGAGGACAAAAGAAAGAACTTGAAAGGTACAACAAAAATTTA
  GAAGAAGCTAAAAGAATTGGGATAAAGAAAGCTATTACAGCCAATATTTCTATAGGTGCT
  GCTTTCCTGCTGATCTATGCATCTTATGCTCTGGCCTTCTGGTATGGGACCACCTTGGTC
  CTCTCAGGGGAATATTCTATTGGACAAGTACTCACTGTATTCTTTTCTGTATTAATTGGG
  GCTTTTAGTGTTGGACAGGCATCTCCAAGCATTGAAGCATTTGCAAATGCAAGAGGAGCA
  GCTTATGAAATCTTCAAGATAATTGATAATAAGCCAAGTATTGACAGCTATTCGAAGAGT
  GGGCACAAACCAGATAATATTAAGGGAAATTTGGAATTCAGAAATGTTCACTTCAGTTAC
  CCATCTCGAAAAGAAGTTAAGATCTTGAAGGGTCTGAACCTGAAGGTGCAGAGTGGGCAG
  ACGGTGGCCCTGGTTGGAAACAGTGGCTGTGGGAAGAGCACAACAGTCCAGCTGATGCAG
  AGGCTCTATGACCCCACAGAGGGGATGGTCAGTGTTGATGGACAGGATATTAGGACCATA
  AATGTAAGGTTTCTACGGGAAATCATTGGTGTGGTGAGTCAGGAACCTGTATTGTTTGCC
  ACCACGATAGCTGAAAACATTCGCTATGGCCGTGAAAATGTCACCATGGATGAGATTGAG
  AAAGCTGTCAAGGAAGCCAATGCCTATGACTTTATCATGAAACTGCCTCATAAATTTGAC
  ACCCTGGTTGGAGAGAGAGGGGCCCAGTTGAGTGGTGGGCAGAAGCAGAGGATCGCCATT
  GCACGTGCCCTGGTTCGCAACCCCAAGATCCTCCTGCTGGATGAGGCCACGTCAGCCTTG
  GACACAGAAAGCGAAGCAGTGGTTCAGGTGGCTCTGGATAAGGCCAGAAAAGGTCGGACC
  ACCATTGTGATAGCTCATCGTTTGTCTACAGTTCGTAATGCTGACGTCATCGCTGGTTTC
  GATGATGGAGTCATTGTGGAGAAAGGAAATCATGATGAACTCATGAAAGAGAAAGGCATT
  TACTTCAAACTTGTCACAATGCAGACAGCAGGAAATGAAGTTGAATTAGAAAATGCAGCT
  GATGAATCCAAAAGTGAAATTGATGCCTTGGAAATGTCTTCAAATGATTCAAGATCCAGT
  CTAATAAGAAAAAGATCAACTCGTAGGAGTGTCCGTGGATCACAAGCCCAAGACAGAAAG
  CTTAGTACCAAAGAGGCTCTGGATGAAAGTATACCTCCAGTTTCCTTTTGGAGGATTATG
  AAGCTAAATTTAACTGAATGGCCTTATTTTGTTGTTGGTGTATTTTGTGCCATTATAAAT
  GGAGGCCTGCAACCAGCATTTGCAATAATATTTTCAAAGATTATAGGGGTTTTTACAAGA
  ATTGATGATCCTGAAACAAAACGACAGAATAGTAACTTGTTTTCACTATTGTTTCTAGCC
  CTTGGAATTATTTCTTTTATTACATTTTTCCTTCAGGGTTTCACATTTGGCAAAGCTGGA
  GAGATCCTCACCAAGCGGCTCCGATACATGGTTTTCCGATCCATGCTCAGACAGGATGTG
  AGTTGGTTTGATGACCCTAAAAACACCACTGGAGCATTGACTACCAGGCTCGCCAATGAT
  GCTGCTCAAGTTAAAGGGGCTATAGGTTCCAGGCTTGCTGTAATTACCCAGAATATAGCA
  AATCTTGGGACAGGAATAATTATATCCTTCATCTATGGTTGGCAACTAACACTGTTACTC
  TTAGCAATTGTACCCATCATTGCAATAGCAGGAGTTGTTGAAATGAAAATGTTGTCTGGA
  CAAGCACTGAAAGATAAGAAAGAACTAGAAGGTTCTGGGAAGATCGCTACTGAAGCAATA
  GAAAACTTCCGAACCGTTGTTTCTTTGACTCAGGAGCAGAAGTTTGAACATATGTATGCT
  CAGAGTTTGCAGGTACCATACAGAAACTCTTTGAGGAAAGCACACATCTTTGGAATTACA
  TTTTCCTTCACCCAGGCAATGATGTATTTTTCCTATGCTGGATGTTTCCGGTTTGGAGCC
  TACTTGGTGGCACATAAACTCATGAGCTTTGAGGATGTTCTGTTAGTATTTTCAGCTGTT
  GTCTTTGGTGCCATGGCCGTGGGGCAAGTCAGTTCATTTGCTCCTGACTATGCCAAAGCC
  AAAATATCAGCAGCCCACATCATCATGATCATTGAAAAAACCCCTTTGATTGACAGCTAC
  AGCACGGAAGGCCTAATGCCGAACACATTGGAAGGAAATGTCACATTTGGTGAAGTTGTA
  TTCAACTATCCCACCCGACCGGACATCCCAGTGCTTCAGGGACTGAGCCTGGAGGTGAAG
  AAGGGCCAGACGCTGGCTCTGGTGGGCAGCAGTGGCTGTGGGAAGAGCACAGTGGTCCAG
  CTCCTGGAGCGGTTCTACGACCCCTTGGCAGGGAAAGTGCTGCTTGATGGCAAAGAAATA
  AAGCGACTGAATGTTCAGTGGCTCCGAGCACACCTGGGCATCGTGTCCCAGGAGCCCATC
  CTGTTTGACTGCAGCATTGCTGAGAACATTGCCTATGGAGACAACAGCCGGGTGGTGTCA
  CAGGAAGAGATTGTGAGGGCAGCAAAGGAGGCCAACATACATGCCTTCATCGAGTCACTG
  CCTAATAAATATAGCACTAAAGTAGGAGACAAAGGAACTCAGCTCTCTGGTGGCCAGAAA
  CAACGCATTGCCATAGCTCGTGCCCTTGTTAGACAGCCTCATATTTTGCTTTTGGATGAA
  GCCACGTCAGCTCTGGATACAGAAAGTGAAAAGGTTGTCCAAGAAGCCCTGGACAAAGCC
  AGAGAAGGCCGCACCTGCATTGTGATTGCTCACCGCCTGTCCACCATCCAGAATGCAGAC
  TTAATAGTGGTGTTTCAGAATGGCAGAGTCAAGGAGCATGGCACGCATCAGCAGCTGCTG
  GCACAGAAAGGCATCTATTTTTCAATGGTCAGTGTCCAGGCTGGAACAAAGCGCCAGTGA
  ACTCTGACTGTATGAGATGTTAAATACTTTTTAATATTTGTTTAGATATGACATTTATTC
  AAAGTTAAAAGCAAACACTTACAGAATTATGAAGAGGTATCTGTTTAACATTTCCTCAGT
  CAAGTTCAGAGTCTTCAGAGACTTCGTAATTAAAGGAACAGAGTGAGAGACATCATCAAG
  TGGAGAGAAATCATAGTTTAAACTGCATTATAAATTTTATAACAGAATTAAAGTAGATTT
  TAAAAGATAAAATGTGTAATTTTGTTTATATTTTCCCATTTGGACTGTAACTGACTGCCT
  TGCTAAAAGATTATAGAAGTAGCAAAAAGTATTGAAATGTTTGCATAAAGTGTCTATAAT
  AAAACTAAACTTTCATGTGACTGGAGTCATCTTGTCCAAACTGCCTGTGAATATATCTTC
  TCTCAATTGGAATATTGTAGATAACTTCTGCTTTAAAAAAGTTTTCTTTAAATATACCTA
  CTCATTTTTGTGGGAATGGTTAAGCAGTTTAAATAATTCCTGTTGTATATGTCTATTCAC
  ATTGGGTCTTACAGAACCATCTGGCTTCATTCTTCTTGGACTTGATCCTGCTGATTCTTG
  CATTTCCACATTAAGGTGGCTCTCAAACCCAAAACACAGATCGATATAAGATTTTAGGAG
  GTGGGGTGAGGCACAATTATGAAGTGCATATTATTCAGATGTATAGTAAATACTTTGCAT
  TGCTTTTATAAAGGGTTCTTTAAGGATTTTTTAAGATTTTTACACTTCCAAAGTACATTT
  ACTTTTTGTCTTGATAATGAATCACTTTTGCCAGTAATTGGCCTCTTCCAAAAGTGATCC
  TTCTGACTTACAGATGTTTAAAATGTGCCATGAGTTTAACTACATCTTGATACAAACATG
  ATAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000927 unedited
  AGAAACTAGCGATTTTGTNAATACGNACTTCACTATAGGGNNCGGCCGCGCAATTCGGCA
  CGAGGCAAGCTCAAAGAAGCAGAGGCCGCTGTTCGTTTCCTTTAGGTCTTTCCACTAAAG
  TCGGAGTATCTTCTTCCAAAATTTCACGTCTTGGTGGCCGTTCCAAGGAGCGCGAGGTCG
  GGATGGATCTTGAAGGGGACCGCAATGGAGGAGCAAAGAAGAAGAACTTTTTTAAACTGA
  ACAATAAAAGTGAAAAAGATAAGAAGGAAAAGAAACCAACTGTCAGTGTATTTTCAATGT
  TTCGCTATTCAAATTGGCTTGACAAGTTGTATATGGTGGTGGGAACTTTGGCTGCCATCA
  TCCATGGGGCTGGACTTCCTCTCATGATGCTGGTGTTTGGAGAAATGACAGATATCTTTG
  CAAATGCAGGAAATTTAGAAGATCTGATGTCAAACATCACTAATAGAAGTGATATCAATG
  ATACAGGGTTCTTCATGAATCTGGAGGAAGACATGACCAGGTATGCCTATTATTACAGTG
  GAATTGGTGCTGGGGTGCTGGTTGCTGCTTACATTCAGGTTTCATTTTGGTGCCTGGCAG
  CTGGAAGACAAATACACAAAATTAGAAAACAGTTTTTTCATGCTATAATGCGACAGGAGA
  TAGGCTGGTTTGATGTGCACGATGTTGGGGAGCTTAACACCCGACTTACAGATGATGTCT
  CCAAGATTAATGAAGNGAATTGGGTGACAAATTGGAATGTTNCTTTCAGTCAATGGCAAC
  ATTTTTCACTGGGTTTATAGTAGGATTTACACGTGGTTTGAAGCTACCCCTTGTGATTTT
  GGCCATCAGTCCCTGTCTTGGACTGTCAACTGCTGTCTGGGCAAAGATCTATCTTCACTT
  ACTGATAAAGAACTCTTAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000927 unedited
  AATGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATCATGTTTGT
  ATCAAGATGTAGTTAAACTCATGGCACATTTTAAACATCTGTAAGTCAGAAGGATCACTT
  TTGGAAGAGGCCAATTACTGGCAAAAGTGATTCATTATCAAGACAAAAAGTAAATGTACT
  TTGGAAGTGTAAAAATCTTAAAAAATCCTTAAAGAACCCTTTATAAAAGCAATGCAAAGT
  ATTTACTATACATCTGAATAATATGCACTTCATAATTGTGCCTCACCCCACCTCCTAAAA
  TCTTATATCGATCTGTGTTTTGGGTTTGAGAGCCACCTTAATGTGGAAATGCAAGAATCA
  GCAGGATCAAGTCCAAGAAGAATGAAGCCAGATGGTTCTGTAAGACCCAATGTGAATAGA
  CATATACAACAGGAATTATTTAAACTGCTTAACCATTCCCACAAAAATGAGTAGGTATAT
  TTAAAGAAAACTTTTTTAAAGCAGAAGTTATCTACAATATTCCAATTGAGAGAAGATATA
  TTCACAGGCAGTTTGGACAAGATGACTCCAGTCACATGAAAGTTTAGTTTTATTATAGAC
  ACTTTATGCAAACATTTCAATACTTTTTGCTACTTCTATAATCTTTTAGCAAGGCAGTCA
  GTTACAGTCCAAATGGGAAAATATAAACAAAATTACACATTTTATCTTTTAAAATCTACT
  TTAATTCTGTTATAAAATTTATAATGCAGTTTAAACTATGATTTCTCTCCACTTGATGAT
  GTCTCTCACTCTGTTCCCTTAATTACGAAGTCTCTGAAGACTCTGAACTTGACTGAGGAA
  TGTTAAACAGAACCTCCTCATAATCTGAAGTGTTTGCTTTTAACTTTGATAAATGTCTAT
  CTAACCAAATTAAAAGTATTTACATCTATACAGTCGGAGTCACTGGCGCTTTGTTCCACC
  TGGCACTGACCATTGAAAAAATGCCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000927
• Insert Size: 4900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone has been fully sequenced and the ORF associated with this clone was found to be a perfect match to NM_000927.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000927.3, NP_000918.2
• RefSeq Size: 4872 bp
• RefSeq ORF: 3843 bp
• Locus ID: 5243
• UniProt ID: P08183
• Cytogenetics: 7q21.12
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]
  Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Variants 2, 3, and 4 all encode the same isoform (2).