COX7A1 (NM_001864) Human Untagged Clone

SKU SC118989

COX7A1 (untagged)-Human cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) (COX7A1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: COX7A1
• Synonyms: COX7A; COX7AH; COX7AM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118989 sequence for NM_001864 edited (data generated by NextGen Sequencing); ATGCAGGCCCTTCGGGTGTCCCAGGCGCTGATCCGCTCCTTCAGCTCCACCGCCCGGAAC; CGCTTTCAGAACCGAGTGCGCGAGAAACAGAAGCTCTTCCAGGAGGACAATGACATCCCG; TTGTACCTGAAGGGCGGCATCGTTGACAACATCCTGTACCGAGTGACAATGACGCTGTGT; CTGGGCGGCACTGTCTACAGCTTGTACTCCCTTGGCTGGGCCTCCTTCCCCAGGAATTAA; ; Clone variation with respect to NM_001864.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001864 unedited; NGCGTTCGGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGACAAGG; CAGAATGCAGGCCCTTCGGGTGTCCCAGGCGCTGATCCGCTCCTTCAGCTCCACCGCCCG; GAACCGCTTTCAGAACCGAGTGCGCGAGAAACAGAAGCTCTTCCAGGAGGACAATGACAT; CCCGTTGTACCTGAAGGGCGGCATCGTTGACAACATCCTGTACCGAGTGACAATGACGCT; GTGTCTGGGCGGCACTGTCTACAGCTTGTACTCCCTTGGCTGGGCCTCCTTCCCCAGGAA; TTAAGACCAAGAAGCCTGGGGGGCCTGAGAGACTTGAACAAGTGTCAATAAACGCTGGCC; TCTGAAAAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAG; CTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGG; CCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCA; TTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGTGGAGGGGGGGTTGGGNNNNNNC; NNNNNNNNNNNCNNNNCNNCCNNNCNNNNNNNNNNNNNCNNCNNNCCCCCCNCCCCCNCC; CCCCCCCCCCCCCCCCCCCCCNNCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCC; CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCCCCCCC; CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCTTCCCCCCCCCCCC; CCCCCCCCCCCCCACCCCCNCCCCCCCCCCNNCNCNNCCNCCCCCNNNCCCCCCCCNCCC; CCCCCCCCCCCCCCCCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001864 unedited; GCGCGCCGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTCAGAGGCCAGC; GTTTATTGACACTTGTTCAAGTCTCTCAGGCCCCCCAGGCTTCTTGGTCTTAATTCCTGG; GGAAGGAGGCCCAGCCAAGGGAGTACAAGCTGTAAACAGTGCCGCCCAAACACAGCGTCA; TTGTCACTCGGTACAGGATGTTGTCAACGATGCCGCCCTTCAGGTACAACGGGATGTCAT; TGTCCTCCTGGAAGAGCTTCTGTTTCTCGCGCACTCGGTTCTGAAAGCGGTTCCGGGCGG; TGGAGCTGAAGGAGCGGATCAGCGCCTGGGACACCCGAAGGGCCTGCATTCTGCCTTGTC; CCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCA; CTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCA; ACGGGGCGGNGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAAC; TCCCATTGACGTCAATGGGGTGGAGACTTGGAATTCCCCGTGAGTCAAACCGCTATCCAC; GCCCATTGGTGTACTGCCCAAACCGCATCACCATGGTAATAGCGATGACTAATACGTANA; TGTACTGCCAAGTAGGAAAGTCCCGTAAGGTCATGTACTGGGCATAATGCCAAGCGGGCC; ATTTACCGTCATTGACGTCAATAGGGGGGCGGACTTGGCATATGATACACTTGATGGACT; GGCCAGTGGGCAGTTTACCGGAATACTCCCCCCATTGACGTCAATGGAAGGCCCTATTGC; GTTCCTTGGAAACATACCTATTATTGANTCAATGGCCGGGGTTTTGGGCGGCAACCCGCG; GGCTTTACCCGAAGTTTGTACCCGGACTCCATATTGGCATTGACTATGACCCGTAATGAT; TCTANTAAACTGGCATATCATGCAACTGCGGGCATTTGACTGGGCATTANTGCCTTTTGA; TTAAAACACGTTGCATGGCATACCATTTGTTTTGCTTAACC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001864
• Insert Size: 380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001864.2, NP_001855.1
• RefSeq Size: 783 bp
• RefSeq ORF: 240 bp
• Locus ID: 1346
• UniProt ID: P24310
• Cytogenetics: 19q13.12
• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Oxidative phosphorylation, Parkinson's disease
• Summary: Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (muscle isoform) of subunit VIIa and the polypeptide 1 is present only in muscle tissues. Other polypeptides of subunit VIIa are present in both muscle and nonmuscle tissues, and are encoded by different genes. [provided by RefSeq, Jul 2008]